本文選題：阿爾茨海默癥　切入點(diǎn)：電針　出處：《中國(guó)針灸》2017年06期 　論文類型：期刊論文

【摘要】：目的:觀察不同頻率電針"百會(huì)"與"腎俞"穴對(duì)阿爾茨海默病(AD)模型大鼠學(xué)習(xí)記憶能力和海馬組織糖原合酶激酶-3β(GSK-3β)、生長(zhǎng)相關(guān)蛋白-43(GAP-43)的影響,探討不同頻率電針防治AD的作用機(jī)制。方法:將112只Wistar雄性大鼠按隨機(jī)數(shù)字表法分為正常組、假手術(shù)組、模型組、針刺組、2 Hz電針組、30 Hz電針組、50 Hz電針組,每組16只。正常組實(shí)驗(yàn)室常規(guī)飼養(yǎng),不進(jìn)行任何處理;假手術(shù)組于雙側(cè)海馬齒狀回注射0.9%Na Cl溶液;其他組采用雙側(cè)海馬齒狀回注射β-淀粉樣蛋白1-42(Aβ1-42)制備AD模型。造模成功15 d后,模型組、假手術(shù)組不進(jìn)行任何處理,2 Hz、30 Hz、50 Hz電針組大鼠選"百會(huì)""腎俞"穴進(jìn)行相應(yīng)頻率電針治療,每日1次,7次為一療程,療程間休息1 d,共治療2個(gè)療程。針刺組選穴與電針組相同,但僅針刺不接電針。治療結(jié)束后立即進(jìn)行水迷宮檢測(cè),記錄大鼠逃避潛伏期、首次跨越平臺(tái)時(shí)間、跨越平臺(tái)次數(shù),并分別采用免疫組化法和Western blot檢測(cè)大鼠海馬組織GSK-3β、GAP-43的表達(dá)。結(jié)果:(1)水迷宮測(cè)試示,與正常組比較,模型組大鼠逃避潛伏期、首次跨越平臺(tái)時(shí)間延長(zhǎng)(均P0.01),跨越平臺(tái)次數(shù)明顯減少(P0.01)。針刺組、3組電針組與模型組比較,逃避潛伏期、首次跨越平臺(tái)的時(shí)間縮短(均P0.01),跨越平臺(tái)次數(shù)明顯增加(P0.01)。50 Hz電針組與針刺組、2 Hz、30 Hz電針組比較,逃避潛伏期縮短(P0.01,P0.05)、首次跨越平臺(tái)時(shí)間縮短(均P0.01),跨越平臺(tái)次數(shù)明顯增加(P0.01,P0.05)。(2)與正常組比較,模型組GSK-3β、GAP-43表達(dá)明顯增多(均P0.01)。與模型組比較,針刺組和3組電針組GSK-3β表達(dá)明顯減少(均P0.01),GAP-43表達(dá)明顯增多(均P0.01)。與針刺組、2 Hz、30 Hz電針組比較,50 Hz電針組GSK-3β表達(dá)明顯減少,GAP-43表達(dá)增加(均P0.01)。結(jié)論:電針可能通過下調(diào)GSK-3β、上調(diào)GAP-43表達(dá),從而促進(jìn)突觸損傷修復(fù),改善AD大鼠的學(xué)習(xí)記憶能力。50 Hz電針治療效果優(yōu)于30 Hz、2 Hz電針組及針刺組。
[Abstract]:Objective: to observe the effects of electroacupuncture of Baihui and Shenshu on learning and memory ability and glycogen synthase kinase-3 尾 -GSK-3 尾 -43 GAP-43 in hippocampus of rats with Alzheimer's disease (AD). Methods: 112 male Wistar rats were randomly divided into normal group, sham operation group, model group and acupuncture group. 16 rats in each group were fed in the normal laboratory without any treatment, and 0.9 NaCl solution was injected into the dentate gyrus of both sides in the sham operation group. The AD model was established by injecting 尾 -amyloid protein 1-42A 尾 1-42 into the dentate gyrus of the other groups. After 15 days of successful modeling, the model group was treated with 尾 -amyloid protein 1-42A 尾 1-42. Rats in the sham operation group were treated with "Baihui" and "Shenshu" at the corresponding frequency, 7 times a day as a course of treatment. The acupuncture group selected points the same as the electroacupuncture group, but only the acupuncture group did not receive electroacupuncture. After the treatment, the water maze test was carried out immediately, the escape latency of rats was recorded, the first time of crossing the platform, the times of crossing the platform, the time of crossing the platform, the time of crossing the platform for the first time, and the number of times of crossing the platform. Immunohistochemical method and Western blot were used to detect the expression of GSK-3 尾 -GAP-43 in hippocampus of rats. Results compared with the normal group, the escape latency of the model group was higher than that of the normal group. The time of crossing the platform was prolonged for the first time (all P0.01A, the times of crossing the platform were significantly decreased P0.01.The escape latency of EA group and model group was compared with that of model group. The time of crossing the platform for the first time was shortened (all P 0.01), and the times of crossing the platform were significantly increased compared with the acupuncture group and the acupuncture group. Compared with the normal group, the expression of GSK-3 尾 -GAP-43 in the model group was significantly higher than that in the normal group (P 0.01). The expression of GSK-3 尾 in acupuncture group and electroacupuncture group was significantly decreased (P 0.01) and the expression of GAP-43 was significantly increased (all P 0.01). Compared with acupuncture group, the expression of GSK-3 尾 in 50 Hz electroacupuncture group decreased significantly (all P 0.01). Conclusion: electroacupuncture may increase the expression of GSK-3 尾 (P 0.01). Regulation of GSK-3 尾, up-regulation of GAP-43 expression, The results showed that the effect of electroacupuncture on learning and memory ability of AD rats was better than that of 30 Hz / 2 Hz electroacupuncture group and 30 Hz / 2 Hz electroacupuncture group, so as to promote the repair of synaptic injury and improve the learning and memory ability of AD rats.
【作者單位】： 湖北中醫(yī)藥大學(xué);湖北中醫(yī)藥大學(xué)針灸骨傷學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目:81373741
【分類號(hào)】：R245

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