本文選題：刺柏葉　切入點(diǎn)：液相指紋圖譜　出處：《內(nèi)蒙古醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:本課題以蒙藥材刺柏葉為研究對(duì)象,建立液相指紋圖譜結(jié)合一測(cè)多評(píng)(QAMS)的質(zhì)量控制模式和氣相指紋圖譜分析方法,為全面控制刺柏葉的內(nèi)在質(zhì)量提供理論依據(jù);并以刺柏葉中的穗花杉雙黃酮、扁柏雙黃酮為指標(biāo),建立以刺柏葉入藥的蒙醫(yī)經(jīng)典方藥“吉祥安坤丸”的含量測(cè)定方法,為刺柏葉在蒙醫(yī)臨床應(yīng)用提供科學(xué)依據(jù)。方法:(1)采用高效液相色譜法,以穗花杉雙黃酮為參照物,對(duì)12批不同來(lái)源的刺柏葉進(jìn)行指紋圖譜測(cè)定,并建立穗花杉雙黃酮與槲皮苷、竹柏雙黃酮A、扁柏雙黃酮的相對(duì)校正因子,通過(guò)相對(duì)校正因子計(jì)算槲皮苷、竹柏雙黃酮A、扁柏雙黃酮的含量,并與外標(biāo)法測(cè)得值進(jìn)行比較。(2)采用氣相色譜法,以α-蒎烯為參照物,對(duì)10批不同來(lái)源的刺柏葉進(jìn)行指紋圖譜測(cè)定。(3)采用高效液相色譜法,對(duì)吉祥安坤丸中刺柏葉所含主要成分穗花杉雙黃酮和扁柏雙黃酮進(jìn)行含量測(cè)定研究。結(jié)果:(1)建立了刺柏葉液相指紋圖譜共有模式,標(biāo)定了16個(gè)共有峰,指認(rèn)了其中5個(gè)共有峰:茵芋苷、槲皮苷、穗花杉雙黃酮、竹柏雙黃酮A、扁柏雙黃酮,12批刺柏葉的相似度均在0.95以上;穗花杉雙黃酮與槲皮苷、竹柏雙黃酮A、扁柏雙黃酮的相對(duì)校正因子(RCF)依次為2.080、1.383和1.077,相對(duì)保留時(shí)間(RRT)依次為0.559、1.254和1.303,一測(cè)多評(píng)法和外標(biāo)法所得槲皮苷、竹柏雙黃酮A、扁柏雙黃酮的含量基本一致,相對(duì)誤差均未超過(guò)1.08%。(2)建立了刺柏葉氣相指紋圖譜共有模式,標(biāo)定了13個(gè)共有峰,10批刺柏葉的相似度均不小于0.90。(3)建立了吉祥安坤丸中穗花杉雙黃酮和扁柏雙黃酮的含量測(cè)定方法,穗花杉雙黃酮和扁柏雙黃酮的線性范圍分別為0.0366~0.366μg,r=0.9999和0.0262~0.262μg,r=0.9999;平均回收率分別為100.9%,RSD=1.22%和99.7%,RSD=1.81%(n=6)。結(jié)論:液相指紋圖譜與一測(cè)多評(píng)相結(jié)合的質(zhì)量控制模式用于評(píng)價(jià)刺柏葉的內(nèi)在質(zhì)量即全面準(zhǔn)確又具有專屬性;以刺柏葉揮發(fā)性成分為主的氣相指紋圖譜可與液相指紋圖譜的信息互為補(bǔ)充,更好的評(píng)價(jià)藥材的品質(zhì);吉祥安坤丸中的穗花杉雙黃酮和扁柏雙黃酮均為刺柏葉的主要活性成分,所建立的含量測(cè)定方法簡(jiǎn)單易行,可為刺柏葉用于吉祥安坤丸的定量研究提供參考。
[Abstract]:Objective: to establish a quality control model and a gas phase fingerprint analysis method based on liquid phase fingerprint and QAMS to provide a theoretical basis for the overall control of the internal quality of Cypress chinensis leaves. The content determination method of "Jixiang Ankun pills", a classical Mongolian medicine, was established on the basis of the index of Chinese fir bisflavone and cypress diflavonoids in the leaves of Cypress chinensis, and the content of them was determined by the traditional Mongolian medicine "Jixiang Ankun Pill". To provide scientific basis for the clinical application of cypress leaf in Mongolian medicine. Methods the fingerprint of 12 batches of Chinese cypress leaves from different sources was determined by high performance liquid chromatography (HPLC) and quercetin from different sources were established. The contents of quercetin, cypress bisoflavone A and cypress diflavonoids were calculated by relative correction factor, and compared with those obtained by external standard method. Using 偽 -pinene as the reference material, 10 batches of Chinese cypress leaves from different sources were determined by HPLC. The contents of bisflavone and bisflavone in the leaves of Cypress chinensis were determined in Jixiang Ankun Pill. Results the common pattern of liquid phase fingerprint of Cypress chinensis leaves was established, and 16 common peaks were calibrated. Among them, five common peaks were identified: the similarity of the leaves of 12 batches of Artemisia chinensis, quercetin, fir bisflavone, cypress bisoflavone A and cypress diflavone were all above 0.95, and the similarity between them was more than 0.95, and the similarity between them was more than 0.95, and the similarity between them was above 0.95. The relative correction factors (RCFs) were 2.080 and 1.077, and the relative retention time (RRTT) were 0.559n1.254 and 1.303, respectively. The contents of quercetin, diflavonoids A and diflavonoids were basically the same as those obtained by multi-evaluation method and external standard method. The common pattern of gas phase fingerprint of cypress leaf was established, and the similarity of 10 batches of Cypress chinensis in 13 common peaks was calibrated. The similarity was not less than 0.90.93) the method for the determination of bisflavone and diflavone in Jixiang Ankun Pill was established. The linear ranges of bisflavone and cypress bisflavone were 0.0366n 0.366 渭 g ~ 0.9999 and 0.0262 ~ 0.262 渭 g ~ 0.262 渭 g ~ (0.9999), respectively. The average recoveries were 100.9% RSD1. 22% and 99. 7% RSD1. 81% respectively. Conclusion: the quality control model combined with liquid fingerprint and multiple evaluation was used to evaluate the internal quality of leaves of Cypress przewalskii. It is comprehensive, accurate and exclusive. The gas phase fingerprint with the volatile components in the leaves of Cypress chinensis can complement the information of the liquid fingerprint, and better evaluate the quality of the medicinal materials, and the bisflavone of Chinese fir in Jixiang Ankun pills is the main active components of the leaves of Cypress przewalskii. The established method is simple and practical and can be used for quantitative study of Jixiang Ankun pills.
【學(xué)位授予單位】：內(nèi)蒙古醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R29

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