本文關(guān)鍵詞： 瑤藥銅鉆 總黃酮 心肌缺血再灌注 抗炎 抗氧化 細(xì)胞凋亡　出處：《桂林醫(yī)學(xué)院》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:研究瑤藥銅鉆(Mappianthus iodoies Hand-Mazz)總黃酮對(duì)急性心肌缺血再灌注損傷的保護(hù)作用。方法:以72只成年健康雄性SD大鼠作為研究對(duì)象,隨機(jī)將其平均分為假手術(shù)組(Sham)、再灌注模型組(Model)、陽性藥物對(duì)照組(YXDZ)、瑤藥銅鉆總黃酮低劑量組(YYTZ-L 60 mg·kg-1)、瑤藥銅鉆總黃酮中劑量組(YYTZ-M 120 mg·kg-1)和瑤藥銅鉆總黃酮高劑量組(YYTZ-H 240 mg·kg-1)六個(gè)組。結(jié)扎大鼠左冠狀動(dòng)脈前降支造成缺血30 min、解除結(jié)扎形成再灌注24 h的方法建立缺血再灌注模型。通過HE染色法對(duì)大鼠心肌組織病理形態(tài)學(xué)改變進(jìn)行觀察;用TTC染色法檢測(cè)大鼠心肌梗死面積;ELISA試劑盒檢測(cè)大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、腫瘤壞死因子-α(TNF-α)、白介素-6(IL-6)、肌酸激酶(CK)、乳酸脫氫酶(LDH)的濃度;免疫印跡檢測(cè)B細(xì)胞淋巴瘤/白血病-2(BCL-2)和BCL-2相關(guān)X蛋白(BAX)蛋白表達(dá)。結(jié)果:與Model相比,瑤藥銅鉆總黃酮?jiǎng)┝拷M對(duì)大鼠急性心肌缺血再灌注損傷的心肌組織病理形態(tài)學(xué)損害均有明顯改善,心肌梗死面積明顯較小(P0.05),血清中MDA、TNF-α、IL-6、CK、LDH含量均較低(P0.05),SOD含量較高(P0.05),BCL-2蛋白水平表達(dá)升高、BAX蛋白水平表達(dá)降低(P0.05);與Sham相比,Model心肌組織病理形態(tài)學(xué)損傷較明顯,心肌梗死面積明顯較大(P0.05),大鼠血清中CK、LDH、MDA、TNF-α、IL-6含量均明顯增高(P0.05),SOD含量較低(P0.05),大鼠BCL-2蛋白水平表達(dá)降低、BAX蛋白水平表達(dá)升高(P0.05)。結(jié)論:瑤藥銅鉆總黃酮對(duì)急性心肌缺血再灌注損傷有一定的保護(hù)作用,其機(jī)制可能與其抗炎、抗氧化及對(duì)抑制細(xì)胞凋亡作用有關(guān)。
[Abstract]:Objective: to study the iodoies Hand-Mazzs of Chinese herbal medicine Mappianthus iodoies Hand-Mazz. The protective effects of total flavonoids on acute myocardial ischemia-reperfusion injury were studied in 72 adult male SD rats. They were randomly divided into sham-operated group, reperfusion model group, and positive drug control group (YXDZ). YYTZ-L 60 mg 路kg-1). YYTZ-M (120 mg 路kg-1) and YYTZ-H (240 mg 路kg -1). The left anterior descending coronary artery was ligated for 30 min. The ischemia reperfusion model was established by removing ligation and forming reperfusion for 24 h. The pathological changes of myocardium in rats were observed by HE staining. Myocardial infarction area was measured by TTC staining. Serum superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor- 偽 (TNF- 偽), interleukin-6 (IL-6) were detected by ELISA kit. The concentration of creatine kinase (CK) and lactate dehydrogenase (LDH); The expression of B-cell lymphoma / Leukemia BCL-2) and BCL-2 associated X protein was detected by Western blotting. Results: compared with Model. The total flavonoid dosage group of Yao medicine copper drill significantly improved the myocardial histopathologic damage of acute myocardial ischemia-reperfusion injury in rats, the myocardial infarction area was significantly smaller than that of P0.05, and MDA in serum was also significantly improved. The content of TNF- 偽, IL-6, CKL and LDH was higher than that of P0.05 and SOD, and the expression of BCL-2 protein was higher than that of P0.05 and BCL-2. The expression of BAX protein decreased in P0.05; Compared with Sham, myocardial histopathologic damage was more obvious, myocardial infarction area was significantly larger than P0.05, and serum CKD LDHDA-TNF- 偽 was found in rats. The content of IL-6 was significantly higher than that of P0.05, and the expression of BCL-2 protein was decreased in rats. Conclusion: the total flavonoids of Chinese herbal medicine have protective effect on acute myocardial ischemia-reperfusion injury, and its mechanism may be related to its anti-inflammatory mechanism. [WT5 "HZ] [WT5" HZ] [WT5 "BZ] [WT5BZ] the expression of BAX protein is increased. Antioxidation and inhibition of apoptosis.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R29

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 胡彥武;劉凱;閆夢(mèng)彤;蔣金凌;張洋;王亞帝;張敏;任立群;;淫羊藿總黃酮及淫羊藿苷的心血管保護(hù)作用及機(jī)制研究進(jìn)展[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2015年13期

2 徐曉娜;牛子冉;王守寶;陳俞材;高莉;方蓮花;杜冠華;;牛舌草總黃酮抗大鼠心肌缺血再灌注損傷的作用及機(jī)制[J];藥學(xué)學(xué)報(bào);2014年06期

3 Ling Xue;Zhen Wu;Xiao-Ping Ji;Xia-Qing Gao;Yan-Hua Guo;;Effect and mechanism of salvianolic acid B on the myocardial ischemiareperfusion injury in rats[J];Asian Pacific Journal of Tropical Medicine;2014年04期

4 李菁;侯飛燕;李榮東;龍憲軍;;金銀花中總黃酮微波輔助提取工藝優(yōu)化及其含量動(dòng)態(tài)變化[J];中南藥學(xué);2014年01期

5 邵瑩;吳啟南;周婧;樂巍;巢建國;;淡竹葉黃酮對(duì)大鼠心肌缺血/再灌注損傷的保護(hù)作用[J];中國藥理學(xué)通報(bào);2013年02期

6 常天輝,劉曉陽,章新華,王懷良;前胡甲素對(duì)缺血再灌注心肌IL-6水平及Fas,bax,bcl-2蛋白表達(dá)的影響(英文)[J];Acta Pharmacologica Sinica;2002年09期

7 周麗娜,戴斌;瑤藥銅鉆(定心藤)的生藥鑒定[J];廣西中醫(yī)藥;2002年02期

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