本文關(guān)鍵詞：咖啡因拮抗針刺抗炎癥痛效應(yīng)及下丘腦疼痛信號(hào)分子的表達(dá)　出處：《成都中醫(yī)藥大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 咖啡因 電針鎮(zhèn)痛 炎癥痛 疼痛信號(hào)分子 下丘腦

【摘要】：目的：研究咖啡因?qū)ρ装Y痛針刺鎮(zhèn)痛效應(yīng)及下丘腦疼痛相關(guān)分子表達(dá)的影響。方法：以C57BL/6J小鼠為研究對(duì)象,篩選基礎(chǔ)熱痛閾值在10 s～20 s的小鼠,隨機(jī)分為空白組、模型組、模型+電針組(簡(jiǎn)稱(chēng)模針組)、模型+電針+咖啡因組(簡(jiǎn)稱(chēng)模咖針組)；使用完全弗氏佐劑足底注射制備炎癥痛模型；模咖針組攝入70 mg/Kg/day咖啡因,空白組、模型組、模針組攝入飲用水；模針組、�？п樈M進(jìn)行電針干預(yù),2/100Hz疏密波,0.2mA,15分鐘,1次／日,連續(xù)干預(yù)11天；采用輻射熱測(cè)痛法測(cè)定各組痛閾,治療結(jié)束后處死動(dòng)物取下丘腦,用PCR Array高通量檢測(cè)技術(shù)檢測(cè)下丘腦疼痛相關(guān)分子的表達(dá)。結(jié)果：(1)咖啡因?qū)ρ装Y痛針刺鎮(zhèn)痛效應(yīng)的影響結(jié)果：組間比較：造模后3天,與空白組比較,模型組、模針組以及�？п樈M小鼠痛閾明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.000),而模型組、模針組以及�？п樈M三組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),具有可比性。在造模后7天,模針組與模型組痛閾比較差異有統(tǒng)計(jì)學(xué)意義(P0.000)；模咖針組與模型組痛閾比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)；�？п樈M與模針組比較差異有統(tǒng)計(jì)學(xué)意義(P0.000)。在造模后13與14天,模針組與模型組痛閾比較差異有統(tǒng)計(jì)學(xué)意義(PO.000),�？п樈M與模型組痛閾比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),模咖針組與模針組比較差異有統(tǒng)計(jì)學(xué)意義(P0.001)。組內(nèi)比較：模型組在造模后7天的痛閡與造模后3天比較有所改善(P0.001)；在造模13與14天的痛閾與造模后3天比較差異有統(tǒng)計(jì)學(xué)意義(P0.000)；在造模13與14天的痛閾與造模后7天比較差異有統(tǒng)計(jì)學(xué)意義(PO.000)；而第13和14天的痛閾差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。模針組在造模后7天的痛閾與造模后3天比較差異有統(tǒng)計(jì)學(xué)意義(P0.000)；模針組在造模13與14天的痛閾與造模后7天比較異有統(tǒng)計(jì)學(xué)意義(P0.000)；模針組在造模13與14天的痛閾比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。�？п樈M在造模后7天的痛閾與造模后3天比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)；模咖針組在造模13與14天的痛閾與造模后7天比較差異有統(tǒng)計(jì)學(xué)意義(P0.001)；模咖針組在第13天與第14天痛閾比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。(2)下丘腦內(nèi)疼痛相關(guān)分子mRNA的表達(dá)：與空白相比較,模型組有18個(gè)基因明顯,其中表達(dá)上調(diào)有13個(gè),下調(diào)有5個(gè)；與模型組比較,模針組基因有7個(gè)基因變化明顯,其中表達(dá)下降有1個(gè),表達(dá)上調(diào)有6個(gè)；與模型組比較,模咖針組有25基因變化明顯：表達(dá)上調(diào)有24個(gè),表達(dá)下調(diào)有1個(gè)；與模針組比較,模咖針組有25基因變化明顯,其中表達(dá)上調(diào)有24個(gè),表達(dá)下調(diào)有1個(gè)。其中,與咖啡因影響電針鎮(zhèn)痛相關(guān)的基因是Ngf。與空白組相比,模型組中基因Ngf的表達(dá)上調(diào),上調(diào)倍數(shù)為1.9053；與模型組相比,模針組中Ngf基因的表達(dá)下調(diào),下調(diào)倍數(shù)為0.4475；與模型組相比,�？п樈M中基因Ngf變化不明顯,其值為0.7253。結(jié)論：(1)電針對(duì)炎癥痛有確切的鎮(zhèn)痛效應(yīng)；(2)電針期間攝入咖啡因(70 mg/Kg/day),可抑制電針的鎮(zhèn)痛效應(yīng)；(3)咖啡因攝入減弱電針鎮(zhèn)痛效應(yīng)可能與下丘腦Ngf基因的表達(dá)有關(guān)。
[Abstract]:Objective: To study the expression effect of caffeine on inflammatory pain and the analgesic effect of acupuncture and pain related hypothalamic molecules. Methods: C57BL/6J mice as the research object, screening based thermal pain threshold at 10 s ~ 20 s mice were randomly divided into control group, model group, model + EA group (referred to as the EA group, model +) electroacupuncture + caffeine group (referred to as die coffee needle group); preparation of inflammatory pain model using intraplantar injection of complete Freund's adjuvant; model acupuncture group 70 mg/Kg/day caffeine coffee intake, blank group, model group, EA group drinking water; electro acupuncture group, model group of electric needle needle coffee intervention, 2/100Hz density wave 0.2mA, 15 minutes, 1 times per day, 11 days of continuous intervention; the radiant heat was measured the pain threshold measured pain, after the end of treatment were used to detect the expression of animal in the hypothalamus Array pain PCR high-throughput detection technology related molecules. Results: (1) caffeine on inflammatory pain The effect of acupuncture analgesia: comparison between groups: 3 days after modeling, compared with the blank group, model group, electro acupuncture group and acupuncture group die coffee pain threshold of mice decreased significantly, the difference was statistically significant (P0.000), and model group, EA group and coffee needle group differences between the three groups was not statistically meaning (P0.05), comparable. In the model after 7 days, there was statistical significance of EA group and model group (P0.000); pain threshold difference was not statistically significant. Coffee needle group and the model group difference threshold (P0.05); the difference mode coffee needle group and electro acupuncture group had statistical significance (P0.000). At 13 and 14 days, there was statistical significance of EA group and model group (PO.000), the pain threshold difference was not statistically significant. Coffee needle group and model group (P0.05), the pain threshold difference there was statistically significant difference between the model group and EA group coffee needle group (P0.001). Comparison: in the rats of model group 鍚，

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