長(zhǎng)鏈非編碼RNA H19在食管鱗癌中的表達(dá)水平及其臨床預(yù)后相關(guān)性的研究
發(fā)布時(shí)間：2021-07-06 07:12
食管癌是最致命的惡性腫瘤之一。它是導(dǎo)致腫瘤死亡的第六大原因,也是世界上第八大最常見的腫瘤。主要是因?yàn)樗鼧O難早期診斷,侵襲能力強(qiáng),因此預(yù)后差以及存活率低。其五年存活率約為15%-25%。更糟糕的是,隨著世界范圍內(nèi)人口老齡化的增長(zhǎng),食管癌的發(fā)病率也正在逐年上升。中國(guó)是食管癌發(fā)病率最高的地區(qū)之一,尤其以中國(guó)河南省安陽(yáng)市最高。長(zhǎng)鏈非編碼RNA（long non-coding RNA,lncRNA）為長(zhǎng)度大于200個(gè)核苷酸,不具有編碼蛋白功能的RNA分子。報(bào)道表明,它可以通過與DNA、染色質(zhì)、蛋白質(zhì)、RNA等大分子相互作用促進(jìn)或抑制腫瘤的發(fā)生、侵襲和轉(zhuǎn)移,其復(fù)雜的作用機(jī)制是目前研究的重點(diǎn)和難點(diǎn)。據(jù)報(bào)道,LncRNAH19在成人組織中表達(dá)量低或不表達(dá),但在多種腫瘤中高表達(dá),如大腸癌、乳腺癌、膀胱癌等,且與腫瘤的發(fā)生發(fā)展密切相關(guān)。因此LncH19有可能具有腫瘤早期診斷和預(yù)后判斷的標(biāo)志物的臨床價(jià)值。本課題大規(guī)模地使用ISH方法檢測(cè)食管鱗癌中H19的表達(dá)水平,旨在探討其在食管癌中的臨床病理作用。其次運(yùn)用生物信息學(xué)分析方法預(yù)測(cè)LncRNA H19的靶向miRNA,探討H19在食管鱗癌中可能的作用機(jī)制。方法...
【文章來源】：鄭州大學(xué)河南省 211工程院校
【文章頁(yè)數(shù)】：65 頁(yè)
【學(xué)位級(jí)別】：碩士
【部分圖文】：
圖１?Ｈ１９原位雜交??
?實(shí)驗(yàn)結(jié)果???相關(guān)，見圖２。??＾?１?Ｃ３?水１５一年內(nèi)死?Ｃ?？〇－，?１?＊＊：４??ｆＭ－?…?■■艦??＇：ＵＵ?＂？?Ｉｉ〇Ｉｊ?ｊ?ｊＩ??圖２?ＥＳＣＣ樣本中ＬｎｃＨｌ９?ＩＳＨ表達(dá)水平柱狀圖??注：Ａ：兩個(gè)ＥＳＣＣ組中ＬｎｃＨｌ９ＩＳＨ表達(dá)水平差異＾?Ｂ：ＬｎｃＨｌ９表達(dá)水平與Ｕ１ＣＣ分期??的關(guān)系。＂＊?ｐ?＝?０．〇０ｌ。??表３?ＬｎｃＲＮＡ?ＨＩ９表達(dá)與食管鱗癌臨床病理特點(diǎn)之間的關(guān)系??臨床特征?總?弱陽(yáng)性?中等陽(yáng)性?強(qiáng)陽(yáng)性?Ｐ值??（Ｎ＝ｌ２ｌ）?（Ｎ＝ｌ９）?ｆＮ＝６４）?（Ｎ＝３８）??生存分組?０．００丨ａ??術(shù)后一年內(nèi)死亡（％）?６３（５２．１）?６（５．０）?１２（９．９）?４５（３７．２）??術(shù)后超過五年生存（％）?５８（４７．９）?２０（１６．５）?２０（１６．５）?１８（１４．９）??年齡?０．１３２ｂ??＞６０（％）?６０（４９．６）?１３（１０．７）?１５（１２．４）?３２（２６．４）??＜６０（％）?６１（５０．４）?１３（１０．７）?１７（１４．０）?３１（２５．６）??性別?０．５９１ｃ??女（％）?６３（５２．１）?１５（１２．４）?１８（１４．９）?３０（２４．８）??男（％）?５８（４７．９）?１?１（９．１）?１４（１１．６）?３３（２７．３）??腫瘤大小?０．００２ａ??＞３ｃｍ（％）?１０７（８８．４）?１８（１４．９）?３０（２４．８）?５９（４８．８）??＜３ｃｍ（％）?１４（１１．６）?８（６．６）?２（１．７）?４（３．３）??ＵＩＣＣ?分期?０．００?ｌｃ??ＰＴ１（％）?１６（１３．２）
圖３韋恩圖??
【參考文獻(xiàn)】：
期刊論文
[1]Metastasis-associated long noncoding RNAs in gastrointestinal cancer: Implications for novel biomarkers and therapeutic targets[J]. Fei-Fei Zhang,Yu-Hao Luo,Hui Wang,Liang Zhao. World Journal of Gastroenterology. 2016(39)
[2]Esophageal cancer: Risk factors,screening and endoscopic treatment in Western and Eastern countries[J]. María José Domper Arnal,ángel Ferrández Arenas,ángel Lanas Arbeloa. World Journal of Gastroenterology. 2015(26)
[3]Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?[J]. Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate Institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of Pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China. World Journal of Gastroenterology. 2010(24)
本文編號(hào)：3267785
【文章來源】：鄭州大學(xué)河南省 211工程院校
【文章頁(yè)數(shù)】：65 頁(yè)
【學(xué)位級(jí)別】：碩士
【部分圖文】：
圖１?Ｈ１９原位雜交??
?實(shí)驗(yàn)結(jié)果???相關(guān)，見圖２。??＾?１?Ｃ３?水１５一年內(nèi)死?Ｃ?？〇－，?１?＊＊：４??ｆＭ－?…?■■艦??＇：ＵＵ?＂？?Ｉｉ〇Ｉｊ?ｊ?ｊＩ??圖２?ＥＳＣＣ樣本中ＬｎｃＨｌ９?ＩＳＨ表達(dá)水平柱狀圖??注：Ａ：兩個(gè)ＥＳＣＣ組中ＬｎｃＨｌ９ＩＳＨ表達(dá)水平差異＾?Ｂ：ＬｎｃＨｌ９表達(dá)水平與Ｕ１ＣＣ分期??的關(guān)系。＂＊?ｐ?＝?０．〇０ｌ。??表３?ＬｎｃＲＮＡ?ＨＩ９表達(dá)與食管鱗癌臨床病理特點(diǎn)之間的關(guān)系??臨床特征?總?弱陽(yáng)性?中等陽(yáng)性?強(qiáng)陽(yáng)性?Ｐ值??（Ｎ＝ｌ２ｌ）?（Ｎ＝ｌ９）?ｆＮ＝６４）?（Ｎ＝３８）??生存分組?０．００丨ａ??術(shù)后一年內(nèi)死亡（％）?６３（５２．１）?６（５．０）?１２（９．９）?４５（３７．２）??術(shù)后超過五年生存（％）?５８（４７．９）?２０（１６．５）?２０（１６．５）?１８（１４．９）??年齡?０．１３２ｂ??＞６０（％）?６０（４９．６）?１３（１０．７）?１５（１２．４）?３２（２６．４）??＜６０（％）?６１（５０．４）?１３（１０．７）?１７（１４．０）?３１（２５．６）??性別?０．５９１ｃ??女（％）?６３（５２．１）?１５（１２．４）?１８（１４．９）?３０（２４．８）??男（％）?５８（４７．９）?１?１（９．１）?１４（１１．６）?３３（２７．３）??腫瘤大小?０．００２ａ??＞３ｃｍ（％）?１０７（８８．４）?１８（１４．９）?３０（２４．８）?５９（４８．８）??＜３ｃｍ（％）?１４（１１．６）?８（６．６）?２（１．７）?４（３．３）??ＵＩＣＣ?分期?０．００?ｌｃ??ＰＴ１（％）?１６（１３．２）
圖３韋恩圖??
【參考文獻(xiàn)】：
期刊論文
[1]Metastasis-associated long noncoding RNAs in gastrointestinal cancer: Implications for novel biomarkers and therapeutic targets[J]. Fei-Fei Zhang,Yu-Hao Luo,Hui Wang,Liang Zhao. World Journal of Gastroenterology. 2016(39)
[2]Esophageal cancer: Risk factors,screening and endoscopic treatment in Western and Eastern countries[J]. María José Domper Arnal,ángel Ferrández Arenas,ángel Lanas Arbeloa. World Journal of Gastroenterology. 2015(26)
[3]Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?[J]. Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate Institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of Pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China. World Journal of Gastroenterology. 2010(24)
本文編號(hào)：3267785
本文鏈接：http://www.sikaile.net/yixuelunwen/zlx/3267785.html
最近更新
教材專著
