【摘要】：研究目的:1、研究四跨膜蛋白Tspan9對(duì)胃癌SGC7901細(xì)胞遷移和侵襲的影響;2、觀察Tspan9對(duì)整合素β1蛋白表達(dá)的影響;3、驗(yàn)證Tspan9是否通過(guò)整合素β1影響SGC7901細(xì)胞的遷移和侵襲。研究方法:1、首先檢測(cè)前期通過(guò)慢病毒轉(zhuǎn)染Tspan9的T-SGC7901細(xì)胞中Tspan9蛋白的表達(dá)情況;2、通過(guò)劃痕實(shí)驗(yàn)和transwell實(shí)驗(yàn)分別檢測(cè)Tspan9對(duì)SGC7901細(xì)胞遷移和侵襲的影響;3、通過(guò)脂質(zhì)體瞬時(shí)轉(zhuǎn)染整合素β1,建立ITG/T-SGC7901細(xì)胞,并檢測(cè)轉(zhuǎn)染后整合素β1的表達(dá)情況;4、再次通過(guò)細(xì)胞劃痕實(shí)驗(yàn)和transwell實(shí)驗(yàn)檢測(cè)轉(zhuǎn)染整合素β1后SGC7901細(xì)胞遷移和侵襲能力的變化。結(jié)果:1、與對(duì)照組相比,T-SGC7901細(xì)胞Tspan9蛋白的表達(dá)明顯增多(P0.05);2、劃痕實(shí)驗(yàn)顯示,T-SGC7901細(xì)胞的遷移率較對(duì)照組明顯降低(P0.05),transwell實(shí)驗(yàn)顯示,T-SGC7901細(xì)胞侵入到下室的細(xì)胞數(shù)量較對(duì)照組明顯減少(P0.05);3、轉(zhuǎn)染整合素β1后,ITG/T-SGC7901細(xì)胞整合素β1的表達(dá)明顯增多;4、劃痕實(shí)驗(yàn)顯示,ITG/T-SGC7901細(xì)胞的遷移率較對(duì)照組明顯加快(P0.05),transwell實(shí)驗(yàn)顯示,ITG/T-SGC7901細(xì)胞侵入到下室的細(xì)胞數(shù)量較對(duì)照組明顯增多(P0.05)。結(jié)論和意義:Tspan9能夠抑制胃癌細(xì)胞SGC7901的遷移和侵襲能力,這種抑制作用可能是通過(guò)下調(diào)整合素β1表達(dá)實(shí)現(xiàn)的。本研究進(jìn)一步加深了對(duì)四跨膜蛋白和惡性腫瘤關(guān)系的認(rèn)識(shí),為后續(xù)研究提供實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:Aim: 1. To study the effect of four transmembrane protein Tspan9 on the migration and invasion of gastric cancer SGC7901 cells; 2, to observe the effect of Tspan9 on the expression of integrin 尾 1 protein; and 3. To verify whether Tspan9 affects the migration and invasion of SGC7901 cells through integrin 尾 1. Methods: firstly, the expression of Tspan9 protein in T-SGC7901 cells transfected with lentivirus Tspan9 was detected. 2, the effects of Tspan9 on the migration and invasion of SGC7901 cells were detected by scratch test and transwell assay, respectively. 3, ITG/T-SGC7901 cells were established by transient transfer of integrin 尾 1 by liposomes, and the expression of integrin 尾 1 after transfection was detected. 4. The migration and invasiveness of SGC7901 cells were detected by cell scratch test and transwell assay. Results: 1, compared with the control group, the expression of Tspan9 protein in T-SGC7901 cells was significantly increased (P 0.05). 2, scratch test showed that the mobility of T-SGC7901 cells was significantly lower than that in the control group (P 0.05), transwell test). 3, the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly increased after transfection of integrin 尾 1 compared with the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05). 4, scratch test showed that the mobility of ITG/T-SGC7901 cells was significantly faster than that of the control group (P 0.05), transwell test showed that the number of ITG/T-SGC7901 cells invading the inferior chamber was significantly higher than that of the control group (P 0.05). Conclusion and significance: Tspan9 can inhibit the migration and invasion of SGC7901 in gastric cancer cells, which may be achieved by down-regulating the expression of integrin 尾 1. This study further deepened the understanding of the relationship between four transmembrane proteins and malignant tumors, and provided an experimental basis for further study.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R735.2

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