【摘要】：目的:探討SRPX2在人子宮內(nèi)膜癌中的表達(dá)及生物學(xué)功能。方法:收集50例子宮內(nèi)膜癌及癌旁組織,免疫組化染色法檢測(cè)SRPX2的表達(dá),分析SRPX2表達(dá)與患者臨床特點(diǎn)的關(guān)系。通過(guò)si RNA下調(diào)HEC-1A細(xì)胞中SRPX2的表達(dá),檢測(cè)HEC-1A細(xì)胞增殖及凋亡改變。檢測(cè)沉默SRPX2后細(xì)胞內(nèi)FAK及Cyclin D1表達(dá)改變。結(jié)果:SRPX2在子宮內(nèi)膜癌組織中表達(dá)明顯升高并與腫瘤直徑增大及高FIGO分期(Ⅲ+Ⅳ期)顯著相關(guān)(P0.05);沉默SRPX2表達(dá)可顯著抑制HEC-1A細(xì)胞的增殖并促進(jìn)凋亡,并導(dǎo)致細(xì)胞中FAK及Cyclin D1的表達(dá)降低。結(jié)論:子宮內(nèi)膜癌組織中高表達(dá)的SRPX2與腫瘤增殖關(guān)系密切,沉默SRPX2可能通過(guò)下調(diào)FAK/Cyclin D1的表達(dá)而抑制子宮內(nèi)膜癌細(xì)胞增殖并促進(jìn)凋亡。
[Abstract]:Objective: to investigate the expression and biological function of SRPX2 in human endometrial carcinoma. Methods: the expression of SRPX2 was detected by immunohistochemical staining in 50 cases of endometrial carcinoma and paracancerous tissues, and the relationship between the expression of SRPX2 and the clinical characteristics of the patients was analyzed. The expression of SRPX2 in HEC-1A cells was down-regulated by si RNA, and the proliferation and apoptosis of HEC-1A cells were detected. The expression of FAK and Cyclin D1 in cells after silencing SRPX2 was detected. Results: the expression of SRPX2 was significantly increased in endometrial carcinoma and significantly correlated with the increase of tumor diameter and high FIGO stage (stage III IV) (P 0.05). The expression of silent SRPX2 could significantly inhibit the proliferation and promote apoptosis of HEC-1A cells, and lead to the decrease of the expression of FAK and Cyclin D1 in the cells. Conclusion: the high expression of SRPX2 in endometrial carcinoma is closely related to tumor proliferation. Silent SRPX2 may inhibit the proliferation and promote apoptosis of endometrial cancer cells by down-regulating the expression of FAK/Cyclin D1.
【作者單位】： 西南醫(yī)科大學(xué)附屬醫(yī)院婦科;
【分類號(hào)】：R737.33
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本文編號(hào)：2508077