【摘要】：近20年的動(dòng)物實(shí)驗(yàn)及臨床前期研究表明,納秒脈沖具有能量高、微創(chuàng)治療、不需聯(lián)合化療藥物等優(yōu)點(diǎn)。因此本研究將納秒脈沖作用于MDA MB 231乳腺癌荷瘤小鼠,觀察局部原發(fā)腫瘤的消融效果,隨訪局部消融對(duì)免疫細(xì)胞亞群的影響情況,探索其非熱消融的免疫機(jī)制。本實(shí)驗(yàn)在小鼠皮下接種小鼠乳腺癌MDA MB 231細(xì)胞,2周腫瘤直徑達(dá)到10 mm時(shí)進(jìn)行納秒脈沖局部消融治療,治療參數(shù)選擇脈沖寬度為300 ns,電場(chǎng)強(qiáng)度為40 k V/cm,脈沖個(gè)數(shù)為100,治療頻率為隔日重復(fù)共3次。研究結(jié)果表明原位接種的MDA MB 231乳腺癌細(xì)胞成瘤率達(dá)到100%。納秒脈沖治療后隨訪腫瘤大小、形態(tài)及體積至1個(gè)月,腫瘤直徑達(dá)到20 mm時(shí)結(jié)束動(dòng)物實(shí)驗(yàn),B超監(jiān)測(cè)數(shù)據(jù)顯示,納秒脈沖局部消融對(duì)原位腫瘤的消融率為83%,顯著降低了原發(fā)腫瘤的體積,與對(duì)照組相比具有顯著性降低;用流式細(xì)胞術(shù)檢測(cè)脾臟中各淋巴細(xì)胞亞群的變化。射頻消融后,小鼠脾臟CD4+T細(xì)胞、CD8+T細(xì)胞數(shù)量和比例升高,提示局部使用的納秒脈沖刺激脾臟T細(xì)胞增殖能力。納秒脈沖消融不但能夠抑制原發(fā)腫瘤,還能增強(qiáng)小鼠免疫功能,使得腫瘤生長(zhǎng)和轉(zhuǎn)移侵襲力下降,有助于抑制腫瘤的生成及發(fā)展。
[Abstract]:Recent 20 years of animal experiments and preliminary clinical studies have shown that nanosecond pulse has the advantages of high energy, minimally invasive therapy and no combination of chemotherapeutic drugs. In this study, nanosecond pulses were applied to MDA MB tumor bearing mice to observe the effect of local primary tumor ablation, to investigate the effect of local ablation on immune cell subsets, and to explore the immune mechanism of non-thermal ablation. In this experiment, mice were inoculated subcutaneously with mouse breast cancer MDA MB 231 cells. Nanosecond pulse ablation was performed at the tumor diameter of 10 mm at 2 weeks. The pulse width of 300 ns, was 40 kV / cm and the number of pulses was 100. The treatment frequency was repeated every other day for 3 times. The results showed that the tumorigenic rate of breast cancer cells inoculated in situ with MDA MB was 100. After nanosecond pulse therapy, the tumor size, shape and volume were followed up to 1 month, and the tumor diameter reached 20 mm. The ultrasound monitoring data showed that the ablation rate of nanosecond pulse local ablation for in situ tumor was 83%. The volume of primary tumor was significantly decreased compared with the control group. The changes of lymphocyte subsets in spleen were detected by flow cytometry. After radiofrequency ablation, the number and proportion of CD4 T cells and CD8 T cells in the spleen of mice were increased, suggesting that the proliferation of spleen T cells was stimulated by nanosecond pulse. Nanosecond pulse ablation can not only inhibit the primary tumor, but also enhance the immune function of mice.
【作者單位】： 浙江大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院乳腺疾病診治中心;浙江大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院肝膽胰外科衛(wèi)生部多器官移植重點(diǎn)實(shí)驗(yàn)室;天津醫(yī)科大學(xué)解剖系;浙江大學(xué)醫(yī)學(xué)院附屬第二醫(yī)院骨科;
【基金】：國(guó)家自然科學(xué)基金(81372425) 浙江省自然科學(xué)基金(LY17H160018) 新疆重點(diǎn)實(shí)驗(yàn)室合作項(xiàng)目(2014KL002)~~
【分類號(hào)】：R737.9
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本文編號(hào)：2422421