【摘要】：目的探討HERC4對宮頸癌細胞生物學功能的影響及其分子機制。方法設(shè)計特異HERC4 si RNA干擾序列,Lipofectamine2000法轉(zhuǎn)染Hela細胞,Western blotting檢測轉(zhuǎn)染特異si RNA后Hela細胞HERC4蛋白的表達水平;CCK-8法檢測轉(zhuǎn)染后Hela細胞的增殖能力;AnnexinⅤ-FITC/PI雙染法檢測轉(zhuǎn)染后Hela細胞凋亡率的變化;劃痕實驗檢測轉(zhuǎn)染后Hela細胞遷移能力。Western blotting檢測轉(zhuǎn)染后Hela細胞Cyclin D1、Bcl-2表達變化。結(jié)果轉(zhuǎn)染特異si RNA后Hela細胞HERC4蛋白表達量明顯下降,差異有統(tǒng)計學意義(P0.01)。與對照組相比,干擾組Hela細胞生長速度明顯減慢,細胞凋亡率增加,遷移能力明顯下降,差異有統(tǒng)計學意義(P0.01)。轉(zhuǎn)染后Cyclin D1和Bcl-2在蛋白水平的表達顯著下調(diào)(P0.01)。結(jié)論 si RNA可有效降低宮頸癌Hela細胞中HERC4的表達,并通過抑制Cyclin D1表達抑制宮頸癌Hela細胞的增殖和遷移能力,抑制Bcl-2表達增加細胞凋亡能力。
[Abstract]:Objective to investigate the effect of HERC4 on the biological function of cervical cancer cells and its molecular mechanism. Methods the specific HERC4 si RNA interference sequence was designed and the expression level of HERC4 protein in Hela cells after transfection of specific si RNA was detected by, Western blotting with Lipofectamine2000 method, and the proliferation ability of Hela cells was detected by CCK-8 method. The apoptosis rate of Hela cells after transfection was detected by Annexin 鈪，

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