【摘要】：目的研究用二氯化鈷(CoCl_2)模擬胃癌細(xì)胞缺氧微環(huán)境的合適濃度,探討內(nèi)皮細(xì)胞特異性分子-1(ESM-1)在胃腺癌SGC-7901/BGC-823細(xì)胞缺氧環(huán)境中的表達(dá)情況。方法以兩種人胃腺癌細(xì)胞(SGC-7901/BGC-823)為研究對(duì)象,分別用不同濃度二氯化鈷(CoCl_2)處理人胃腺癌細(xì)胞后,用四唑化合物(MTS)法檢測(cè)CoCl_2對(duì)SGC-7901和BGC-823細(xì)胞增殖的影響;Western blot分析不同濃度CoCl_2處理的人胃腺癌SGC-7901和BGC-823細(xì)胞中低氧誘導(dǎo)因子-1α(HIF-1α)和ESM-1表達(dá)的變化。結(jié)果≤0.6 mmol/L濃度的CoCl_2對(duì)胃腺癌SGC-7901和BGC-823細(xì)胞的增殖和細(xì)胞活力沒(méi)有明顯影響。在0.5、0.6 mmol/L CoCl_2濃度時(shí),HIF-1α蛋白表達(dá)明顯增加,但ESM-1表達(dá)明顯下降。結(jié)論0.5、0.6 mmol/L CoCl_2可以模擬SGC-7901/BGC-823細(xì)胞的缺氧環(huán)境,在CoCl_20.5、0.6 mmol/L模擬細(xì)胞缺氧環(huán)境中,ESM-1的表達(dá)量與HIF-1α呈負(fù)相關(guān)。
[Abstract]:Objective to study the suitable concentration of cobalt dichloride (CoCl_2) in anoxic microenvironment of gastric cancer cells and to investigate the expression of endothelial cell-specific molecule 1 (ESM-1) in SGC-7901/BGC-823 cells of gastric adenocarcinoma. Methods two kinds of human gastric adenocarcinoma cells (SGC-7901/BGC-823) were treated with different concentrations of cobalt dichloride (CoCl_2). The effects of CoCl_2 on the proliferation of SGC-7901 and BGC-823 cells were detected by (MTS) assay. The expression of hypoxia inducible factor 1 偽 (HIF-1 偽) and ESM-1 in SGC-7901 and BGC-823 cells treated with different concentrations of CoCl_2 were analyzed by Western blot. Results CoCl_2 with concentration 鈮，

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