【摘要】：目的:探討轉(zhuǎn)化生長因子(transforming growth factor-β,TGF-β1)在食管癌中的作用及其對上皮間質(zhì)轉(zhuǎn)化(Epithelial-Mesenchymal Transition,EMT)的調(diào)控。方法:利用TGF-β1受體抑制劑LY2109761處理食管癌Eca109細胞,根據(jù)濃度梯度(0、0.1、1、10μm/ml)分為四組;慢病毒轉(zhuǎn)染TGF-β1干擾RNA(siRNA)處理食管癌Eca109細胞,分為3組:實驗組(病毒穩(wěn)定TGF-β1 si RNA轉(zhuǎn)染Eca109細胞)、陰性對照組(轉(zhuǎn)染無關(guān)序列Eca109細胞)、空白對照組(正常未經(jīng)任何處理的Eca109細胞),采用熒光定量PCR(Quantitative Realtime-PCR,qRT-PCR)技術(shù)檢測TGF-β1及上皮間質(zhì)轉(zhuǎn)化(EMT)相關(guān)指標(biāo)E-鈣粘蛋白(E-cadherin)、波形蛋白(Vimentin)、鋅指轉(zhuǎn)錄因子(Snail)在mRNA水平表達情況;Western blot技術(shù)檢測E-cadherin、Vimentin蛋白水平的表達;同時通過細胞增殖實驗(MTT)、細胞劃痕實驗及Transwell法分別檢測細胞增殖、遷移、侵襲的變化情況。結(jié)果:通過TGF-β1受體抑制劑(0、0.1、1、10μm/ml)處理Eca109細胞后,在mRNA水平TGF-β1表達逐漸降低,E-cadherin表達逐漸升高,Vimentin、Snail表達逐漸降低(P0.05);在蛋白水平,E-cadherin表達逐漸升高,Vimentin表達逐漸降低(P0.05);此外,細胞增殖和遷移能力逐漸降低(P0.05)。通過慢病毒轉(zhuǎn)染TGF-β1 si RNA后發(fā)現(xiàn),實驗組與空白對照組和陰性對照組相比,TGF-β1 mRNA表達降低(P0.05),E-cadherin表達增高,Vimentin表達降低;同時實驗組細胞增殖、遷移和侵襲能力均降低(P0.05)。結(jié)論:TGF-β1可能通過上皮間質(zhì)轉(zhuǎn)化促進食管癌細胞的增殖、遷移和侵襲。
[Abstract]:Aim: to investigate the role of transforming growth factor (transforming growth factor- 尾 1 (TGF- 尾 1) in esophageal carcinoma and its regulation on Epithelial-Mesenchymal transition. Methods: esophageal carcinoma Eca109 cells were treated with TGF- 尾 1 receptor inhibitor (LY2109761) and divided into four groups according to the concentration gradient (0 0. 1 渭 m/ml), lentivirus transfected with TGF- 尾 1 interfered with RNA (siRNA) to treat esophageal cancer Eca109 cells. TGF- 尾 1 was divided into three groups: experimental group (viral stable TGF- 尾 1si RNA transfected Eca109 cells), negative control group (transfection of unrelated Eca109 cells) and blank control group (normal untreated Eca109 cells). TGF- 尾 1 was detected by fluorescence quantitative PCR (Quantitative real time PCR qRT-PCR. Ecadherin (E-cadherin) and (Vimentin), zinc finger transcription factor (Snail) (Snail) were detected by Western blot. At the same time, the cell proliferation, migration and invasion were detected by (MTT), cell scratch assay and Transwell assay. Results: after treated with TGF- 尾 1 receptor inhibitor (0 0. 1 m/ml), the expression of TGF- 尾 1 decreased gradually at the mRNA level and decreased gradually at the mRNA level (P0.05), while at the protein level, the expression of E-cadherin decreased gradually (P0.05), and the expression of Vimentin decreased gradually at the protein level (P0.05). The ability of cell proliferation and migration decreased gradually (P0.05). After lentivirus transfection of TGF- 尾 1si RNA, it was found that the expression of TGF- 尾 1 mRNA in the experimental group was lower than that in the blank control group and the negative control group (P0.05), while the expression of Vimentin in the experimental group was lower than that in the control group (P0.05), and the ability of cell proliferation, migration and invasion in the experimental group was decreased (P0.05). Conclusion TGF- 尾 1 may promote the proliferation, migration and invasion of esophageal carcinoma cells through epithelial interstitial transformation.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R735.1

【相似文獻】

相關(guān)期刊論文 前10條

1 張瑩,張郁,孫葉方,史俊南;TGF-β對人牙乳頭間充質(zhì)細胞堿性磷酸酶的影響[J];牙體牙髓牙周病學(xué)雜志;1999年04期

2 周紅,馬忠森,王秀麗,安繼紅,赫國志;TGF_(β1)和TGF_βR在煤工塵肺大鼠模型肺組織中的表達[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2003年02期

3 宋雅芳,洪嘉禾,徐列明;中醫(yī)藥對TGF-β1調(diào)控作用的研究進展[J];上海中醫(yī)藥雜志;2004年01期

4 鄒貴勉,張t，

本文編號：2179801