【摘要】：目的:探討咖啡酸苯乙酯(phenethyl caffeate,CAPE)對肝細(xì)胞生長因子(hepatocyte growth factor,HGF)誘導(dǎo)的人肝癌細(xì)胞系(Hep G2細(xì)胞)侵襲和遷移的抑制作用及機制。方法:將實驗分為對照組(HGF=0 ng/m L;CAPE=0μmol/L)、誘導(dǎo)組(HGF=20 ng/m L;CAPE=0μmol/L)和荷藥組(HGF=20 ng/m L;CAPE分別為2.5、5.0、7.5、10.0μmol/L),測定細(xì)胞黏附率,Transwell小室模擬細(xì)胞侵襲和遷移能力以及劃痕實驗測定細(xì)胞遷移率,蛋白免疫印跡(Western blot)法檢測ELMO1、MAP4K4和FNDC3B表達(dá)。結(jié)果:與對照組比較,誘導(dǎo)組表現(xiàn)出顯著的促進細(xì)胞黏附和遷移作用,荷藥組中表現(xiàn)出對HGF誘導(dǎo)的細(xì)胞黏附和遷移具有顯著的抑制作用(P0.05)。Transwell小室實驗結(jié)果表明CAPE對HGF誘導(dǎo)的Hep G2細(xì)胞侵襲和遷移作用有抑制作用。Western blot結(jié)果顯示,誘導(dǎo)組和對照組相比ELMO1和MAP4K4表達(dá)顯著上調(diào),荷藥組和誘導(dǎo)組相比ELMO1和MAP4K4表達(dá)顯著下調(diào)(P0.05);誘導(dǎo)組表達(dá)FNDC3B較對照組顯著下降(P0.05),荷藥組FNDC3B表達(dá)量較誘導(dǎo)組增加,但結(jié)果不顯著(P0.05)。結(jié)論:CAPE能夠抑制HGF誘導(dǎo)的Hep G2細(xì)胞的黏附、侵襲和遷移。CAPE通過上調(diào)FNDC3B和下調(diào)ELMO1、MAP4KE的表達(dá),抑制Hep G2細(xì)胞的侵襲和遷移能力。
[Abstract]:Aim: to investigate the inhibitory effect of phenethyl caffeate (Cape) on the invasion and migration of human hepatoma cell line (Hep G2) induced by hepatocyte growth factor (hepatocyte growth). Methods: the experiment was divided into three groups: control group (HGF0 ng/m / L CAPE-0 渭 mol / L), induction group (20 ng/m / L CAPE-0 渭 mol / L) and drug bearing group (20 ng/m / L HGFGF-20 ng/m / L CAPE = 2.5 ng/m / L). The cell adhesion rate and the ability of simulating cell invasion and migration in the transwell compartment were measured by scratch assay. The expression of ELMO1 map4K4 and FNDC3B was detected by Western blot. Results: compared with the control group, the induction group showed significant effects on promoting cell adhesion and migration. The cell adhesion and migration induced by HGF-induced cell adhesion and migration were significantly inhibited in the drug group (P0.05). The results of Transwell chamber experiment showed that Cape could inhibit the invasion and migration of HepG2 cells induced by HGF. Western blot showed that Cape could inhibit the invasion and migration of HGF-induced Hep G2 cells. The expression of ELMO1 and MAP4K4 was significantly up-regulated in the induced group compared with the control group, the expression of ELMO1 and MAP4K4 was down-regulated significantly (P0.05), the expression of FNDC3B in the induced group was significantly lower than that in the control group (P0.05), the expression of FNDC3B in the drug group was higher than that in the induced group, but the results were not significant (P0.05). Conclusion:% Cape can inhibit the adhesion, invasion and migration of HGF induced Hep G2 cells. Cape can inhibit the invasion and migration of Hep G2 cells by upregulating FNDC3B and down-regulating the expression of ELMO1 MAP4KE.
【作者單位】： 南京農(nóng)業(yè)大學(xué)食品科技學(xué)院;中國農(nóng)業(yè)科學(xué)院蜜蜂研究所;
【基金】：中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程專項(CAAS-ASTIP-2015-IAR)
【分類號】：TS201.4;R735.7

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