本文選題：HDAC1 + 胃癌干細胞��； 參考：《安徽醫(yī)科大學》2017年碩士論文

【摘要】：背景與目的:胃癌干細胞是近年來的研究熱點,胃癌干細胞的增殖分化與胃癌的轉移及預后有密切關聯(lián)。組蛋白的乙�；揎椩谂咛ジ杉毎鲋撤只^程中起著非常重要的作用,組蛋白去乙酰化酶1(HDAC1)對胃癌干細胞增值分化的影響及機制未見報導,本研究擬通過慢病毒轉染敲低胃癌干細胞觀察HDAC1表達對人類胃癌干細胞干性增殖、遷移及侵襲的影響以及對胃癌干細胞干性相關基因表達的影響,進一步探討HDAC1低表達對人類胃癌干細胞增殖分化的影響及機制。方法:以Nanog為胃癌細胞干性標志物,流式細胞術篩選出胃癌干細胞。通過慢病毒轉染技術干擾胃癌干細胞中HDAC1表達,實時定量PCR(qRT-PCR)和Western Blot方法檢測干擾效率。采用MTT比色法和劃痕試驗分別觀察兩組細胞增殖和遷移能力的差異;通過transwell實驗觀察兩組細胞侵襲能力的差異;采用RT-qPCR和Western Blot方法檢測兩組細胞干性相關基因表達。結果:HDAC1在實驗組胃癌干細胞中的表達顯著低于對照組(p0.05);實驗組細胞的增殖、遷移和侵襲能力顯著低于對照組(p0.05);實驗組細胞干性相關基因表達顯著低于對照組(p0.05)。結論:HDAC1低表達降低了胃癌干細胞的增殖、遷移及侵襲能力;HDAC1低表達下調了BMi1基因表達可能是其降低胃癌干細胞的增殖、干性及侵襲的機制之一。
[Abstract]:Background & objective: gastric cancer stem cells have been a hot topic in recent years. The proliferation and differentiation of gastric cancer stem cells are closely related to the metastasis and prognosis of gastric cancer. The acetylation modification of histone plays an important role in the proliferation and differentiation of embryonic stem cells. The effect of histone deacetylase 1 (HDAC1) on the proliferation and differentiation of gastric cancer stem cells has not been reported. In this study, the effects of HDAC1 expression on dry proliferation, migration and invasion of human gastric cancer stem cells and the expression of genes related to dry nature of gastric cancer stem cells were observed by lentivirus transfection and low knockout of gastric cancer stem cells. To investigate the effect and mechanism of HDAC1 low expression on the proliferation and differentiation of human gastric cancer stem cells. Methods: gastric cancer stem cells were screened by flow cytometry with Nanog as the dry marker of gastric cancer cells. The expression of HDAC1 in gastric cancer stem cells was inhibited by lentivirus transfection, and the interference efficiency was detected by real-time quantitative PCR (qRT-PCR) and Western blot. MTT colorimetric assay and scratch assay were used to observe the difference of cell proliferation and migration ability, transwell assay was used to observe the difference of cell invasion ability, and RT-qPCR and Western Blot were used to detect the expression of dry-related genes in the two groups. Results the expression of cell weight HDAC1 in gastric cancer stem cells in the experimental group was significantly lower than that in the control group (p0.05), the proliferation, migration and invasion ability of the cells in the experimental group was significantly lower than that in the control group (p0.05), and the expression of dry-related genes in the experimental group was significantly lower than that in the control group (p0.05). Conclusion the low expression of HDAC1 reduces the proliferation of gastric cancer stem cells, and the down-regulation of BMI1 gene expression may be one of the mechanisms that reduce the proliferation, dryness and invasion of gastric cancer stem cells.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R735.2

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相關期刊論文 前9條

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