本文選題：人臍靜脈血管內(nèi)皮細(xì)胞 + 胞質(zhì)多聚腺苷酸結(jié)合蛋白��； 參考：《山東醫(yī)藥》2017年09期

【摘要】：目的探討胞質(zhì)多聚腺苷酸結(jié)合蛋白1(CPEB1)對(duì)人臍靜脈血管內(nèi)皮細(xì)胞(HUVECs)增殖和遷移的影響及可能機(jī)制。方法取對(duì)數(shù)生長(zhǎng)期的HUVECs細(xì)胞,分為實(shí)驗(yàn)組與對(duì)照組,分別用腫瘤細(xì)胞上清液培養(yǎng)基和普通培養(yǎng)基重懸細(xì)胞,Real-time PCR法和Western blotting法分別檢測(cè)CPEB1及整合素-金屬蛋白酶17(ADAM17)的mRNA和蛋白表達(dá);取對(duì)數(shù)生長(zhǎng)期HUVECs細(xì)胞,待培養(yǎng)至細(xì)胞達(dá)70%~80%融合時(shí)用LipofectamineTM2000進(jìn)行轉(zhuǎn)染,細(xì)胞分為攜帶基因的質(zhì)粒轉(zhuǎn)染組(轉(zhuǎn)染組)、不帶任何基因的空質(zhì)粒轉(zhuǎn)染細(xì)胞組(轉(zhuǎn)染對(duì)照組)和不經(jīng)任何處理的細(xì)胞組(空白對(duì)照組)。MTT法檢測(cè)轉(zhuǎn)染后HUVECs的增殖能力,Transwell小室法檢測(cè)轉(zhuǎn)染后HUVECs的遷移能力,RT-PCR法檢測(cè)ADAM17的mRNA表達(dá),Western blotting法檢測(cè)ADAM17蛋白表達(dá)。結(jié)果實(shí)驗(yàn)組中CPEB1的mRNA及蛋白表達(dá)均低于對(duì)照組(P均0.05),ADAM17的mRNA及蛋白表達(dá)均高于對(duì)照組(P均0.05);轉(zhuǎn)染48、72 h后,轉(zhuǎn)染組吸光度值低于轉(zhuǎn)染對(duì)照組和空白對(duì)照組(P均0.05);轉(zhuǎn)染組細(xì)胞穿過(guò)小室膜數(shù)目低于轉(zhuǎn)染對(duì)照組和空白對(duì)照組(P均0.05);轉(zhuǎn)染組ADAMl7的mRNA和蛋白表達(dá)低于轉(zhuǎn)染對(duì)照組和空白對(duì)照組(P均0.05)。結(jié)論 CPEB1可抑制HUVECs細(xì)胞增殖和遷移,其機(jī)制可能與調(diào)控ADAM17表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effect of cytosolic polyadenylate binding protein-1 (CPEB1) on proliferation and migration of human umbilical vein endothelial cells (HUVECs) and its possible mechanism. Methods HUVECs cells in logarithmic growth stage were divided into experimental group and control group. The mRNA and protein expression of CPEB1 and integrin-metalloproteinase-17 (ADAM17) were detected by real-time PCR and Western blotting in tumor cell supernatant medium and ordinary culture medium respectively. HUVECs cells in logarithmic growth phase were transfected with Lipofectamine TM2000 when the cells reached 70% fusion. Cells were divided into plasmid transfection group carrying gene (transfection group), empty plasmid transfection group without any gene (transfection control group) and cell group without any treatment (blank control group). MTT assay was used to detect the proliferation of HUVECs after transfection. MRNA expression of ADAM17 was detected by RT-PCR and the expression of ADAM17 protein was detected by Western blotting. Results the expression of CPEB1 mRNA and protein in the experimental group was lower than that in the control group (P 0.05). The absorbance value of transfection group was lower than that of transfection control group and blank control group (P 0.05), the number of cells passing through ventricular membrane in transfection group was lower than that in transfection control group and blank control group (P 0.05), the mRNA and protein expression of ADAMl7 in transfection group was lower than that in transfection control group. Blank control group (P 0.05). Conclusion CPEB1 can inhibit the proliferation and migration of HUVECs cells, and its mechanism may be related to the regulation of ADAM17 expression.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院;
【基金】：華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢中心醫(yī)院院內(nèi)科研基金項(xiàng)目(YQ14A03)
【分類號(hào)】：R730.2
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本文編號(hào)：2079336