本文選題：bcl-基因 + 結直腸腫瘤��； 參考：《中國病理生理雜志》2017年05期

【摘要】：目的:研究bcl-3基因對人結腸癌RKO細胞遷移及凋亡的影響及機制。方法:采用人bcl-3基因的RNA干擾慢病毒載體沉默人結腸癌RKO細胞bcl-3基因的表達后,劃痕實驗觀察bcl-3基因沉默前后RKO細胞遷移能力的變化,Annexin V/PI雙染色法檢測bcl-3基因沉默前后RKO細胞凋亡率的變化,Western blot法檢測bcl-3基因沉默前后細胞周期蛋白cyclin D1及凋亡相關蛋白Bax、Bcl-2的變化。結果:劃痕實驗顯示,劃痕后36 h,bcl-3基因沉默前后RKO細胞劃痕愈合率分別為84.00%及40.00%,差異具有統(tǒng)計學意義(P0.05)。Annexin V/PI雙染色法流式細胞術分析顯示,bcl-3基因沉默前后的RKO細胞均經(jīng)5μmol/L順鉑處理24 h后,沉默前后的RKO細胞凋亡率分別為12.89%及59.67%,差異具有統(tǒng)計學意義(P0.05)。Western blot法檢測顯示bcl-3基因沉默后cyclin D1蛋白表達顯著下降(P0.05),Bax蛋白表達顯著上升(P0.05),但Bcl-2表達無明顯變化(P0.05)。結論:沉默bcl-3基因后,RKO細胞遷移能力下降,凋亡率增加,并伴細胞周期蛋白cyclin D1及凋亡相關蛋白Bax表達的變化。bcl-3基因可能通過改變cyclin D1及Bax蛋白的表達而影響RKO細胞的凋亡。
[Abstract]:Aim: to study the effect and mechanism of bcl-3 gene on migration and apoptosis of human colon cancer RKO cells. Methods: the expression of bcl-3 gene in human colon cancer RKO cells was silenced by human bcl-3 RNAi lentivirus vector. Effects of bcl-3 gene silencing on the migration of RKO cells and the apoptosis of RKO cells before and after bcl-3 gene silencing by Annexin V / Pi double staining and Western blot assay before and after bcl-3 gene silencing were used to detect the apoptosis of RKO cells and the apoptosis of RKO cells before and after bcl-3 gene silencing. Changes of related protein Baxfen-2. Results: scratch test showed that, The rate of scar healing of RKO cells was 84.00% and 40.005%, respectively, 36 h after the gene silencing. The difference was statistically significant (P 0.05) .Annexin V / Pi double staining flow cytometry analysis showed that the RKO cells were treated with 5 渭 mol / L cisplatin for 24 h before and after the silencing of Bcl-3 gene, and the results showed that RKO cells were treated with 5 渭 mol / L cisplatin for 24 h. The apoptosis rates of RKO cells before and after silencing were 12.89% and 59.67, respectively. The difference was statistically significant (P 0.05). Western blot assay showed that the expression of cyclin D1 protein decreased significantly after bcl-3 gene silencing, and the expression of cyclin D1 protein increased significantly (P 0.05%), but the expression of Bcl-2 did not change significantly (P0.05). Conclusion: after silencing bcl-3 gene, the migration ability of RKO cells decreased and the apoptosis rate increased. With the change of cyclin D1 and Bax expression, bcl-3 gene may affect the apoptosis of RKO cells by changing the expression of cyclin D1 and Bax proteins.
【作者單位】： 寧波大學醫(yī)學院附屬醫(yī)院腫瘤內(nèi)科;寧波市醫(yī)學科學研究所;
【基金】：寧波市社會發(fā)展科研項目(No.2011C50010)
【分類號】：R730.23

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本文編號：2030807