本文選題：地西他濱 + A��； 參考：《中國(guó)醫(yī)科大學(xué)學(xué)報(bào)》2017年12期

【摘要】：目的檢測(cè)地西他濱對(duì)A431細(xì)胞生物學(xué)功能的影響并初步探討其機(jī)制。方法將0、5、10和20μmol/L的地西他濱分別作用于A431細(xì)胞,通過MTT法觀察地西他濱對(duì)細(xì)胞增殖的影響,通過Western blotting和實(shí)時(shí)PCR檢測(cè)地西他濱作用于A431細(xì)胞后細(xì)胞增殖相關(guān)蛋白Cyclin B1、PCNA和CDC2的改變。通過Hoechst 33258染色檢測(cè)0、5和10μmol/L地西他濱對(duì)A431細(xì)胞凋亡狀態(tài)的影響,采用Western blotting和實(shí)時(shí)PCR檢測(cè)地西他濱對(duì)A431細(xì)胞凋亡相關(guān)蛋白Bax和Bcl-2的影響。采用transwell實(shí)驗(yàn)檢測(cè)細(xì)胞侵襲轉(zhuǎn)移情況,并通過Western blotting和實(shí)時(shí)PCR檢測(cè)0、5和10μmol/L的地西他濱對(duì)侵襲轉(zhuǎn)移相關(guān)蛋白MMP2的影響。結(jié)果 MTT檢測(cè)發(fā)現(xiàn),5、10和20μmol/L的地西他濱作用于A431細(xì)胞24 h后對(duì)細(xì)胞增殖的抑制率分別為(43.81±1.53)%、(48.64±4.65)%和(50.69±4.99)%,隨著藥物的濃度增加,對(duì)A431細(xì)胞的增殖抑制率顯著增加(P0.05)。Hoechst 33258染色發(fā)現(xiàn),地西他濱可以顯著促進(jìn)細(xì)胞凋亡,transwell實(shí)驗(yàn)也證明地西他濱可以明顯抑制A431細(xì)胞的轉(zhuǎn)移能力。Western blotting和實(shí)時(shí)PCR結(jié)果顯示,地西他濱影響A431細(xì)胞中Cyclin B1、CDC2、PCNA、Bax、Bcl-2和MMP2蛋白及m RNA水平,地西他濱處理組與對(duì)照組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論地西他濱對(duì)外陰鱗狀細(xì)胞癌的生物學(xué)功能有很強(qiáng)的抑制作用,可以作為治療外陰鱗狀細(xì)胞癌的化療藥物。
[Abstract]:Objective to investigate the effect of dietabine on biological function of A 431 cells and its mechanism. Methods the cell proliferation of A431 cells was observed by MTT, and the changes of Cyclin B1P and CDC2 were detected by Western blotting and real-time PCR. The apoptosis of A431 cells was detected by Hoechst 33258 staining, and the apoptosis related proteins Bax and Bcl-2 of A431 cells were detected by Western blotting and real-time PCR. Transwell assay was used to detect the invasion and metastasis of the cells, and the effects of 0 渭 mol/L and 10 渭 mol/L on the invasion and metastasis associated protein MMP2 were detected by Western blotting and real-time PCR. Results the inhibitory rates of dietabine on A431 cell proliferation were 43.81 鹵1.53 鹵4.65% and 50.69 鹵4.99%, respectively. With the increase of drug concentration, the inhibition rate of proliferation of A431 cells was significantly increased with the increase of drug concentration. Hoechst 33258 staining showed that the inhibitory rate of dietabine on A431 cell proliferation was significantly higher than that of control group. Dietabine could significantly promote apoptosis. The transwell assay also demonstrated that dietabine could significantly inhibit the metastatic ability of A431 cells. Western blotting and real-time PCR results showed that dietabine affected the expression of Bcl-2, MMP2 and m RNA in A431 cells. Compared with the control group, the difference between the two groups was statistically significant (P 0.05). Conclusion Detabine has a strong inhibitory effect on the biological function of vulvar squamous cell carcinoma and can be used as a chemotherapeutic drug for vulvar squamous cell carcinoma.
【作者單位】： 中國(guó)醫(yī)科大學(xué)附屬盛京醫(yī)院婦產(chǎn)科;沈陽醫(yī)學(xué)院附屬第二醫(yī)院普外科;
【基金】：沈陽市科學(xué)技術(shù)計(jì)劃(F15-199-1-34)
【分類號(hào)】：R737.35

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 毛正祿;;地西他濱逆轉(zhuǎn)白血病K562細(xì)胞株多藥耐藥的作用觀察[J];吉林醫(yī)學(xué);2014年02期

，

本文編號(hào)：1982963