本文選題：腫瘤微環(huán)境 + 雙調(diào)蛋白　； 參考：《腫瘤》2017年07期

【摘要】：目的:探討上皮細胞生長因子雙調(diào)蛋白(amphiregulin,AREG)對小鼠腸癌CT26細胞生長的影響及其相關(guān)機制。方法:采用ELISA法檢測不同小鼠腫瘤細胞中AREG蛋白的表達水平。將攜帶AREG基因的重組慢病毒載體轉(zhuǎn)染至小鼠腸癌CT26細胞、黑素瘤B16細胞和肝癌LPC-Akt細胞,同時以轉(zhuǎn)染空載體細胞作為陰性對照。采用MTT法、克隆形成實驗和FCM法分別檢測AREG過表達后細胞的體外增殖和克隆形成能力以及細胞周期進程。構(gòu)建AREG過表達CT26細胞的小鼠移植瘤模型,觀察小鼠體內(nèi)移植瘤的生長情況,并且采用FCM法和實時熒光定量PCR法分別檢測移植瘤組織中免疫細胞的分布情況以及趨化因子的表達水平。結(jié)果:小鼠腸癌CT26、黑素瘤B16和肝癌LPC-Akt細胞中AREG相對低表達。過表達AREG后,上述3種腫瘤細胞的體外增殖能力、克隆形成能力及細胞周期進程均無明顯變化(P值均0.05)。然而,在CT26細胞的小鼠移植瘤模型中,AREG過表達可明顯促進腫瘤細胞的體內(nèi)生長(P0.01),下調(diào)腫瘤組織中CD8~+T細胞所占比例(P0.05),并降低與CD8+T細胞招募相關(guān)的CC趨化因子配體5(CC chemokine ligand 5,CCL5)的轉(zhuǎn)錄水平(P0.05)。結(jié)論:AREG能夠促進小鼠腸癌CT26細胞的體內(nèi)生長,推測其作用機制可能與調(diào)控CD8+T細胞招募相關(guān)趨化因子,從而影響腫瘤微環(huán)境有關(guān)。
[Abstract]:Aim: to investigate the effects of epithelial growth factor (EGF) bimodontin on the growth of CT26 cells in mouse colorectal cancer and its related mechanisms. Methods: ELISA assay was used to detect the expression of AREG protein in different mouse tumor cells. The recombinant lentivirus vector carrying AREG gene was transfected into mouse colorectal cancer CT26 cells, melanoma B16 cells and hepatocellular carcinoma LPC-Akt cells. MTT assay, clone formation assay and FCM assay were used to detect the proliferation and clone formation ability and cell cycle process of AREG overexpression cells in vitro, respectively. A mouse model of transplanted tumor with overexpression of AREG CT26 cells was established to observe the growth of transplanted tumor in mice. The distribution of immunocytes and the expression of chemokines were detected by FCM and real-time quantitative PCR. Results: the expression of AREG in mouse colorectal cancer CT26, melanoma B 16 and hepatoma LPC-Akt cells was relatively low. After overexpression of AREG, the proliferative ability, clone formation ability and cell cycle progression of the above three tumor cells showed no significant change (P = 0.05). However, In mouse transplanted tumor model of CT26 cells, the overexpression of Arg can significantly promote the growth of tumor cells in vivo, down-regulate the proportion of CD8 ~ T cells in tumor tissues and decrease the CC chemokine ligand 5(CC chemokine associated with CD8 T cell recruitment. The transcription level of ligand 5 (CCL5) is P0.05. Conclusion the growth of mouse colorectal cancer CT26 cells can be promoted by WAREG, and the mechanism may be related to the regulation of recruitment of chemokines by CD8 T cells, thus affecting the tumor microenvironment.
【作者單位】： 上海交通大學醫(yī)學院附屬仁濟醫(yī)院上海市腫瘤研究所癌基因及相關(guān)基因國家重點實驗室;
【基金】：國家自然科學基金資助項目(編號:81402348)~~
【分類號】：R73-3

【相似文獻】

相關(guān)期刊論文 前8條

1 桂耀庭;張鍵榮;余振東;唐愛發(fā);李賢新;蔡志明;;雙調(diào)蛋白在人體睪丸組織的表達特征[J];生殖醫(yī)學雜志;2008年02期

2 桂耀庭;張鍵榮;梁婉青;蔡志明;;雙調(diào)蛋白在人體子宮內(nèi)膜組織中的表達特征[J];北京大學學報(醫(yī)學版);2008年03期

3 潘建平;表皮生長因子和雙調(diào)蛋白上調(diào)入乳腹癌細胞基質(zhì)金屬蛋白酶[J];國外醫(yī)學(免疫學分冊);1998年04期

4 馬林;Berthois Y;Calvo F;;雙調(diào)蛋白誘導乳腺癌細胞表達尿激酶型纖溶酶原活化物[J];中國腫瘤臨床;2005年22期

5 劉欣欣;趙江沙;印宏坤;謝東;李文杰;;食管鱗狀細胞癌組織中表皮調(diào)節(jié)素、雙調(diào)蛋白及表皮生長因子受體mRNA的表達[J];鄭州大學學報(醫(yī)學版);2010年02期

6 馬林,Randrianarison V,Calvo F;雙調(diào)蛋白反義重組腺病毒治療乳腺癌的實驗研究[J];中國腫瘤臨床;2004年16期

7 馬林,SEROVA Maria,PODGORNIAK Marie Pierre,BERTHOIS Yolande,MOURAH Samia,CALVO Fabien;雙調(diào)蛋白反義核酸表達對裸鼠乳腺癌血管生成的抑制作用[J];中華腫瘤雜志;2005年09期

8 ;[J];;年期

相關(guān)會議論文 前1條

1 張鍵榮;梁婉青;桂耀庭;蔡志明;;雙調(diào)蛋白在人體子宮內(nèi)膜的表達特征[A];第一屆中華醫(yī)學會生殖醫(yī)學分會、中國動物學會生殖生物學分會聯(lián)合年會論文匯編[C];2007年

相關(guān)碩士學位論文 前1條

1 李文超;尼妥珠單抗聯(lián)合順鉑對宮頸腺癌細胞株hela的作用及其機制的研究[D];南方醫(yī)科大學;2012年

，

本文編號：1919392