本文選題：叉頭框C蛋白 + 肺腺癌��； 參考：《中國老年學雜志》2017年24期

【摘要】：目的探究叉頭框C2蛋白(FOXC2)對肺腺癌細胞惡性增殖及侵襲遷移能力的影響。方法利用慢病毒轉(zhuǎn)染的方法在人肺腺癌細胞系A549中構(gòu)建過表達FOXC2細胞(實驗組)及相應對照細胞系(對照組),Western印跡檢測兩組細胞FOXC2及干性相關分子Oct4、Sox2及Nanog的表達水平,MTT法檢測兩組細胞0、24、48、72、96 h的惡性增殖能力,Transwell侵襲及遷移實驗檢測兩組細胞侵襲遷移能力。結(jié)果與對照組細胞比較,實驗組細胞FOXC2、Oct4、Sox2及Nanog表達水平均顯著上調(diào)(P0.05),細胞惡性增殖能力在48、72及96 h出現(xiàn)顯著升高(P0.05),細胞侵襲及遷移能力均顯著上調(diào)(P0.05),差異均有統(tǒng)計學意義。結(jié)論 FOXC2可促進肺腺癌細胞惡性增殖及侵襲遷移能力,其機制與介導腫瘤細胞干細胞特性形成有關。
[Abstract]:Objective to investigate the effects of forked C2 protein (FOXC2) on the proliferation and invasion and migration of lung adenocarcinoma cells. Methods the expression of FOXC2 cells (experimental group) and corresponding control cell lines (control group) in human lung adenocarcinoma cell line A549 were constructed by lentivirus transfection. The Western blot was used to detect FOXC2 and Oct4 in two groups of cells. The expression level of Sox2 and Nanog, MTT method was used to detect the malignant proliferation ability of 0,24,48,72,96 h in two groups of cells. Transwell invasion and migration test was used to detect the invasion and migration of cells in the two groups. Results compared with the control group, the expression level of FOXC2, Oct4, Sox2 and Nanog in the experimental group were up (P0.05), and the cell malignant proliferation ability was in 48,72 and 96 Significant increase (P0.05), cell invasion and migration ability were significantly up (P0.05), and the difference was statistically significant. Conclusion FOXC2 can promote the malignant proliferation and invasion and migration of lung adenocarcinoma cells, and the mechanism is related to the formation of tumor cell stem cell characteristics.

【作者單位】： 遵義醫(yī)學院附屬醫(yī)院呼吸內(nèi)科;遵義醫(yī)學院附屬醫(yī)院腫瘤科;
【分類號】：R734.2

【相似文獻】

相關期刊論文 前10條

1 崔虎山,李星云,李成福,韓京軍,池永涌;三磷酸腺苷氯化鎂對肺腺癌細胞的抗癌作用[J];延邊大學醫(yī)學學報;2002年03期

2 楊晉平,王澤,張?zhí)K;肺腺癌5-羥色胺受體的免疫組織化學定位[J];四川解剖學雜志;2003年02期

3 吳一龍,林嘉穎,楊學寧,喬貴賓,王坤,陳剛;肺腺癌患者酪氨酸激酶信號傳導通路的異常與臨床預后[J];中華醫(yī)學雜志;2004年12期

4 張利群,陳杭薇,王四海,辛慶紅,杜玉國,尤蘭華;人呼吸道合胞病毒轉(zhuǎn)錄調(diào)節(jié)基因轉(zhuǎn)染肺腺癌細胞系的研究[J];山東醫(yī)藥;2005年12期

5 黎聯(lián);梅同華;周向東;張新高;車德亞;;細胞外信號調(diào)節(jié)激酶信號通路與基質(zhì)金屬蛋白酶-26在肺腺癌表達中的關系[J];第三軍醫(yī)大學學報;2009年02期

6 王亞南;顧國浩;;生物芯片在肺腺癌研究和診療中的應用進展[J];國際檢驗醫(yī)學雜志;2011年02期

7 ;我國人體肺腺癌細胞系建立[J];腫瘤;1981年06期

8 季晨陽,張義R，

本文編號：1886665