本文選題：鼻咽腫瘤 + 注射用黃芪多糖�。� 參考：《實用醫(yī)學雜志》2017年08期

【摘要】：目的:探討注射用黃芪多糖誘導人鼻咽癌細胞株CNE-2的自噬作用。方法:采用CCK8檢測注射用黃芪多糖對CNE-2增殖的抑制作用;吖啶橙染色觀察注射用黃芪多糖作用于CNE-2細胞引起的自噬形態(tài)變化;透射電鏡觀察注射用黃芪多糖作用于CNE-2細胞后引起的細胞超微結構變化。Western blot實驗檢測注射用黃芪多糖作用于CNE-2細胞后Beclin-1的表達變化。結果:注射用黃芪多糖對CNE-2細胞的生長有明顯抑制作用,且具有濃度及時間依賴性。注射用黃芪多糖作用后可誘導CNE-2細胞發(fā)生自噬。透射電鏡下細胞體內大空泡及膜性雙層結構增多;吖啶橙染色可見注射用黃芪多糖作用后觀察到更多的亮紅色酸性自噬泡。Western blot結果表明Beclin-1蛋白表達增高。結論:注射用黃芪多糖抑制細胞增殖,可能通過上調beclin1表達誘導人鼻咽癌細胞CNE-2細胞發(fā)生自噬。
[Abstract]:Objective: To investigate the autophagy effect of injection of Astragalus Polysaccharides on human nasopharyngeal carcinoma cell line CNE-2. Methods: CCK8 was used to detect the inhibitory effect of Astragalus Polysaccharides on CNE-2 proliferation; acridine orange staining was used to observe the changes of autophagy induced by Astragalus Polysaccharide on CNE-2 cells. The transmission electron microscope was used to observe the effect of Astragalus Polysaccharide on CNE-2 The ultrastructural change of cells after the cell.Western blot test was used to detect the expression of Beclin-1 in CNE-2 cells after injection of Astragalus polysaccharides. Results: the injection of Astragalus Polysaccharides had obvious inhibitory effect on the growth of CNE-2 cells, and was dependent on the concentration and time. The injection of Astragalus polysaccharides could induce the occurrence of CNE-2 cells. Autophagy. Under transmission electron microscope, the large vacuoles and membranous double layer structures in the cells in the cells increased; acridine orange staining showed that more bright red acid autophagic vesicles.Western blot results showed that the expression of Beclin-1 protein increased after injection of Astragalus polysaccharides. Conclusion: injection of Astragalus polysaccharide to inhibit cell proliferation may be induced by up regulation of Beclin1 expression. Autophagy occurs in the CNE-2 cells of human nasopharyngeal carcinoma cells.

【作者單位】： 柳州市人民醫(yī)院;南方醫(yī)科大學珠江醫(yī)院腫瘤中心;
【基金】：柳州市人民醫(yī)院院內立項項目(編號:lry201506)
【分類號】：R739.63

【參考文獻】

相關期刊論文 前9條

1 田忠;夏嬙;王新穎;;P13K/Akt信號通路及其在鼻咽癌發(fā)生發(fā)展中的研究進展[J];實用醫(yī)學雜志;2016年04期

2 武有明;張齊;劉永琦;何建新;伍志偉;高卓越;蘇韞;駱亞莉;張利英;周妮娜;;黃芪多糖對肺癌微環(huán)境中BMSCs增殖及TAFs分化的影響[J];中藥藥理與臨床;2015年06期

3 朱丹丹;周大明;趙九軍;;調強放射治療聯(lián)合黃芪多糖注射液治療頭頸部腫瘤的臨床療效[J];實用醫(yī)學雜志;2014年10期

4 石豐榕;汪森明;胡麗娟;曹漫明;范子榮;胡喜鋼;;ApoG2誘導鼻咽癌CNE-2細胞凋亡與自噬的觀察[J];中華腫瘤防治雜志;2013年11期

5 Bo Liu;Jin-Ku Bao;Jin-Ming Yang;Yan Cheng;;Targeting autophagic pathways for cancer drug discovery[J];Chinese Journal of Cancer;2013年03期

6 付俊;尚海旭;賈弘y，

本文編號：1864401