本文選題：mPGES- + K細(xì)胞　； 參考：《中國實(shí)驗(yàn)血液學(xué)雜志》2017年01期

【摘要】：目的:探討抑制前列腺素E2合酶1(mPGES-1)表達(dá)對人急性白血病K562細(xì)胞在裸鼠體內(nèi)成瘤的影響及其可能的機(jī)制。方法:通過shRNA干擾技術(shù)下調(diào)K562細(xì)胞中mPGES-1表達(dá),實(shí)驗(yàn)設(shè)立了如下研究小組:①干擾組(KD),②陰性細(xì)胞(NC)非特異序列shRNA干擾組;③未處理組(CON組);應(yīng)用Western blot法檢測β-catenin和cyclin D1在3組細(xì)胞中的表達(dá);構(gòu)建K562細(xì)胞人-裸鼠移植瘤模型,觀察移植瘤的生長情況;應(yīng)用HE染色觀察各組腫瘤組織的結(jié)構(gòu);應(yīng)用免疫組織化學(xué)法檢測β-catenin和cyclin D1的表達(dá)水平。結(jié)果:與CON組和NC組比較,細(xì)胞體外實(shí)驗(yàn)結(jié)果顯示KD組β-catenin和cyclin D1表達(dá)量減少(P0.05);動物體內(nèi)實(shí)驗(yàn)結(jié)果顯示,KD組瘤體生長明顯減慢,移植瘤體積明顯縮小,重量明顯減輕(P0.01);HE染色顯示,KD組細(xì)胞排列相對松散,間質(zhì)較多,腫瘤細(xì)胞核小,細(xì)胞質(zhì)較少;免疫組織化學(xué)檢測顯示,KD組β-catenin和cyclin D1表達(dá)量明顯下降(P0.05)。結(jié)論:下調(diào)mPGES-1表達(dá)能顯著抑制人急性白血病K562細(xì)胞在裸鼠體內(nèi)成瘤,其機(jī)制可能與抑制β-catenin和cyclinD1表達(dá)相關(guān)。
[Abstract]:Aim: to investigate the effect of inhibition of prostaglandin E 2 synthase 1 mPGES-1 expression on tumorigenesis of human acute leukemia K562 cells in nude mice and its possible mechanism. Methods: shRNA interference technique was used to down-regulate the expression of mPGES-1 in K562 cells. The following research group was established as follows: the control group (1: 1) was used to interfere with the non-specific sequence of shRNA interference in K562 cells. 3The expression of 尾 -catenin and cyclin D1 was detected by Western blot method in untreated group, the model of human xenograft tumor of K562 cells was established, the growth of transplanted tumor was observed, the structure of tumor tissue was observed by HE staining, and the expression of 尾 -catenin and cyclin D1 were detected by HE staining. The expression levels of 尾-catenin and cyclin D1 were detected by immunohistochemistry. Results: compared with CON group and NC group, the expression of 尾 -catenin and cyclin D1 in KD group was decreased in vitro, and the growth of tumor and the volume of transplanted tumor in KD group were significantly decreased in vivo. The expression of 尾 -catenin and cyclin D1 in KD group was significantly decreased by immunohistochemical staining, and the expression of 尾 -catenin and cyclin D1 was significantly decreased in KD group. Conclusion: down-regulation of mPGES-1 expression can significantly inhibit the tumorigenesis of human acute leukemia K562 cells in nude mice, and its mechanism may be related to the inhibition of 尾 -catenin and cyclinD1 expression.
【作者單位】： 中山大學(xué)附屬孫逸仙紀(jì)念醫(yī)院血液內(nèi)科;
【基金】：2012年國家自然青年科學(xué)基金(81200342) 2012年廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金(A2012182) 2014年度廣東省公益研究與能力建設(shè)專項(xiàng)基金項(xiàng)目(2014A020212085)
【分類號】：R733.7

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