本文選題：VX + 經(jīng)肝動(dòng)脈化學(xué)治療栓塞��； 參考：《中南大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年11期

【摘要】：目的:評(píng)價(jià)超順磁性氧化鐵(superparamagnetic iron oxide,SPIO)納米藥物載體聯(lián)合碘化油(lipiodol,LIP)經(jīng)肝動(dòng)脈栓塞治療兔VX2肝癌的可行性及效果。方法:新西蘭大白兔24只,肝內(nèi)種植VX2瘤組織制作VX2肝癌模型,將荷瘤兔隨機(jī)分為阿霉素(DOX)組、DOX-LIP組、SPIO-DOX組、SPIO-DOX-LIP組共4組,每組6只,行肝動(dòng)脈栓塞治療。于術(shù)前以及術(shù)后1,3,5,7 d檢測(cè)血清ALT和AST水平;術(shù)后0,5,15,30,60,120 min檢測(cè)血清DOX濃度;術(shù)后7 d行MRI及CT掃描觀察SPIO及LIP在肝組織及其腫瘤中的分布和沉積情況;術(shù)后7 d取肝及其腫瘤組織檢測(cè)組織DOX濃度,同時(shí)行病理學(xué)檢查,包括HE染色、普魯士蘭染色及TUNEL染色,計(jì)算腫瘤壞死率和凋亡指數(shù)。結(jié)果:與DOX組相比,其他3組術(shù)后1和3 d血清AST和ALT水平均明顯升高(P0.05);術(shù)后7 d DOX組、DOX-LIP組和SPIODOX-LIP組血清AST和ALT水平恢復(fù)至術(shù)前水平,3組間差異無統(tǒng)計(jì)學(xué)意義(P0.05);SPIO-DOX-LIP組術(shù)后120 min內(nèi)各時(shí)間點(diǎn)的血清DOX濃度均明顯低于其他3組(P0.05);SPIO-DOX-LIP組術(shù)后7 d腫瘤組織DOX濃度明顯高于其他3組(P0.05)。術(shù)后7 d T2加權(quán)像(T2WI)示SPIO-DOX組和SPIO-DOX-LIP組腫瘤信號(hào)降低,CT示DOX-LIP組和SPIO-DOX-LIP組腫瘤區(qū)域LIP沉積。術(shù)后7 d病理結(jié)果示SPIO-DOX-LIP組腫瘤中心大部分區(qū)域凝固性壞死,腫瘤壞死率和凋亡指數(shù)均高于其他3組(P0.05)。結(jié)論:SPIO納米藥物載體聯(lián)合LIP是一種安全、可行的化學(xué)治療栓塞體系,具有良好的MRI和CT可視性,可提高兔VX2肝癌的化學(xué)藥物栓塞治療的效果。
[Abstract]:Aim: to evaluate the feasibility and effect of superparamagnetic iron oxidetio (SPIO) nano-drug carrier combined with lipiodolium lipiodolium lipiodol lipiodol (LIP) transcatheter arterial embolization (TACE) in the treatment of rabbit VX2 liver cancer. Methods: 24 New Zealand white rabbits were implanted with VX2 tumor tissue to make VX2 liver cancer model. The rabbits were randomly divided into 4 groups: SPIO-DOX-LIP group (SPIO-DOX-LIP group) and SPIO-DOX-LIP group (6 rabbits in each group). The levels of serum ALT and AST were detected before operation and 7 days after operation, the serum DOX concentration was detected at 0 ~ 5 ~ 1530T ~ 60120 min, and the distribution and deposition of SPIO and LIP in liver tissue and tumor were observed by MRI and CT scanning 7 days after operation. DOX concentration in liver and tumor tissue was measured 7 days after operation. Pathological examination including HE staining Prussian blue staining and TUNEL staining were performed to calculate tumor necrosis rate and apoptosis index. Results: compared with DOX group, The serum AST and ALT levels in the other three groups were significantly increased on the 1st and 3rd day after operation, and the serum AST and ALT levels in the DOX group and the SPIODOX-LIP group returned to the preoperative level on the 7th day after operation. There was no significant difference among the three groups in the level of serum AST and ALT in the DOX group and the SPIODOX-LIP group at each time point within 120 min after the operation in the SPIO-DOX-LIP group. The serum DOX concentration in SPIO-DOX-LIP group was significantly lower than that in the other three groups (P 0.05). The DOX concentration of tumor tissue in SPIO-DOX-LIP group was significantly higher than that in other three groups on the 7th day after operation. On the 7th day after operation, T2 weighted imaging (T2WI) showed decreased tumor signal intensity in SPIO-DOX group and SPIO-DOX-LIP group. Ct showed LIP deposition in tumor area of DOX-LIP group and SPIO-DOX-LIP group. 7 days after operation, the pathological results showed that the coagulation necrosis in most of the tumor center in SPIO-DOX-LIP group was higher than that in the other three groups (P 0.05), and the necrotic rate and apoptosis index were higher than those in the other three groups. ConclusionTwo nano-drug carrier combined with LIP is a safe and feasible chemoembolization system with good MRI and CT visibility. It can improve the effect of chemoembolization of rabbit VX2 liver cancer.
【作者單位】： 中南大學(xué)湘雅三醫(yī)院放射科;中南大學(xué)湘雅藥學(xué)院;
【基金】：湖南省科技計(jì)劃項(xiàng)目(2015SK20664)~~
【分類號(hào)】：R735.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 賈飛鴿;許乙凱;余田;李龍;孫希杰;曲華麗;;大鼠肝癌模型LAC-BSA-SPIO增強(qiáng)MR成像研究[J];放射學(xué)實(shí)踐;2010年04期

2 李易;文曦琳;孫紅武;葛曉東;李妹玲;文明;;超順磁性氧化鐵(SPIO)與多聚賴氨酸(PLL)納米粒的制備、結(jié)構(gòu)驗(yàn)證及其空間結(jié)構(gòu)表征[J];復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版);2014年03期

3 許衛(wèi)國,黃力,陳漢芳,蔣光愉;小鼠移植瘤模型對(duì)SPIO增強(qiáng)MRI信號(hào)變化原因的初步研究[J];臨床放射學(xué)雜志;2005年06期

4 ;Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly(ethylene glycol) and polyethyleneimin[J];Chinese Science Bulletin;2009年18期

5 劉峴,許乙凱,黃其鎏,吳元魁;使用SPIO對(duì)大鼠肝癌模型進(jìn)行MR增強(qiáng)顯像的實(shí)驗(yàn)研究[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2002年12期

6 張雪,徐榮天,任克,孫文閣,戚喜勛,徐克;超順磁性氧化鐵(SPIO)增強(qiáng)MRI對(duì)原發(fā)性肝癌的診斷價(jià)值[J];中國臨床醫(yī)學(xué)影像雜志;2002年04期

7 劉峴,許乙凱,黃其鎏;SPIO與Gd-DTPA聯(lián)合增強(qiáng)檢測(cè)大鼠肝癌病灶的實(shí)驗(yàn)研究[J];中國醫(yī)學(xué)影像學(xué)雜志;2002年06期

8 陽紅艷;許乙凱;吳元魁;劉文源;呂國士;曹國洪;;SPIO增強(qiáng)MRI成像在大鼠脾臟荷VX2腫瘤模型的實(shí)驗(yàn)研究[J];中華腫瘤防治雜志;2008年02期

9 文明;柏瑋;李必波;韓忠;陸云峰;李少林;楊學(xué)恒;;SPIO標(biāo)記的反義探針磁學(xué)性能檢測(cè)[J];重慶大學(xué)學(xué)報(bào);2008年08期

10 張雪輝,梁碧玲,黃穗喬;原發(fā)性肝癌SPIO增強(qiáng)MRI與組織學(xué)分級(jí)的相關(guān)性研究[J];癌癥;2003年07期

相關(guān)會(huì)議論文 前4條

1 徐貴平;佐野美香;市川智章;佐野R較，

本文編號(hào)：1785558