本文選題：免疫組織化學染色 + 辣根過氧化酶�。� 參考：《第三軍醫(yī)大學學報》2017年11期

【摘要】：目的通過對現(xiàn)有的免疫組織化學雙重標記方法(雙標)的改進,探索一種穩(wěn)定且易于辨認的顯色方法,便于在普通光學顯微鏡下觀察肺腺癌組織中腫瘤相關巨噬細胞的分布情況。方法改進的免疫組織化學雙標染色分別使用二抗鏈接的堿性磷酸酶和辣根過氧化物酶作為底物顯色酶,底物分別選用固紅(AP-Red)和二氨基聯(lián)苯胺(DAB),在顯微鏡下次序控制雙標顯色,觀察肺癌組織中腫瘤相關巨噬細胞的分布情況。結(jié)果免疫組織化學雙重標記中第一標記AP-Red的紅色與第二標記DAB的棕黃色反差大,背景低,易于分辨。兩種顏色標記的同一部位呈現(xiàn)磚紅色,易于表示雙標部位。統(tǒng)計顯示在肺腺癌組織標本中,以M2型腫瘤相關巨噬細胞為主,約占80%。結(jié)論改進后的顯色系統(tǒng)建立了簡便、可靠的免疫組織化學雙重標記新方法。標記結(jié)果可以清晰穩(wěn)定顯示腫瘤相關巨噬細胞在肺腺癌組織中分布情況,便于進一步研究其在肺癌發(fā)生、發(fā)展中的作用。
[Abstract]:Objective to explore a stable and easily recognizable method for color development by improving the existing double labeling method (double labeling) of immunohistochemistry.It is convenient to observe the distribution of tumor-associated macrophages in lung adenocarcinoma tissue under general optical microscope.Methods the modified double labeling immunohistochemical staining was performed by using the linked alkaline phosphatase and horseradish peroxidase as the substrate chromogenic enzyme, and the substrate AP-Red and the diaminobenzidine DABX, respectively. The double standard color was controlled in order under the microscope.The distribution of tumor associated macrophages in lung cancer tissues was observed.Results in the double labeling of immunohistochemistry, the red of the first label AP-Red and the brown yellow of the second label DAB had a big contrast, low background and easy to distinguish.The same part of the two color marks appears brick red, easy to represent the double-marked area.Statistics showed that M 2 tumor associated macrophages were predominant in lung adenocarcinoma tissues, accounting for about 80%.Conclusion the improved color system has established a simple and reliable double labeling method for immunohistochemistry.The results of labeling can clearly and stably show the distribution of tumor associated macrophages in lung adenocarcinoma tissues and facilitate further study of the role of tumor associated macrophages in the genesis and development of lung cancer.
【作者單位】： 第三軍醫(yī)大學西南醫(yī)院全軍臨床病理學研究所/西南癌癥中心;
【基金】：國家重點研發(fā)計劃重點專項(2016YFC201801) 國家自然科學基金面上項目(81372273) 西南醫(yī)院生物強院科技創(chuàng)新計劃(SWH2016JCZD-04)~~
【分類號】：R734.2

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