發(fā)布時間：2018-04-10 23:34

  本文選題：化療干預 + 外周血單個核細胞　； 參考：《河北醫(yī)科大學》2015年碩士論文

【摘要】：惡性腫瘤的發(fā)生、發(fā)展與增殖有關(guān)。惡性腫瘤細胞寄生在人體,機體是其賴以生存的內(nèi)環(huán)境。因此,研究外周血單個核細胞的改變有可能為進一步探討惡性腫瘤細胞與機體之間關(guān)系提供理論依據(jù)。外周血單個核細胞,主要包括淋巴細胞及單核細胞,淋巴細胞包括T淋巴細胞、B淋巴細胞及自然殺傷細胞,在免疫監(jiān)視、殺傷靶細胞及免疫調(diào)節(jié)方面具有極其重要的作用,是機體抗腫瘤免疫的重要效應(yīng)細胞,外周血單個核細胞的數(shù)量及功能狀態(tài)反映機體全身免疫機能水平。IL-8是多細胞來源、多功能的細胞因子,能促進炎性反應(yīng)、刺激血管生成、促進有絲分裂、調(diào)節(jié)宿主免疫功能等作用,在多種腫瘤中高表達,與腫瘤發(fā)生的早期事件及腫瘤的進展密切相關(guān)。流式細胞術(shù)是一種在功能水平上對單細胞或其他生物粒子進行定量分析和分選的檢測手段,是當前檢測細胞分化及功能的最先進、最準確的技術(shù),主要應(yīng)用在癌前病變及腫瘤的早期診斷、交叉配型、測定骨髓造血干細胞的重建、淋巴細胞亞群分析、DNA細胞分化和周期的分析及細胞凋亡等方面的檢測。化療是惡性腫瘤的主要治療手段之一,除對惡性腫瘤細胞有殺傷作用,對機體正常組織細胞亦有損傷。目的:探討化療干預對惡性腫瘤患者外周血單個核細胞功能影響。方法:應(yīng)用流式細胞術(shù),對43例惡性腫瘤患者和10例體檢正常健康者晨空腹外周肘靜脈血進行外周血單個核細胞細胞周期、凋亡率及IL-8的檢測。結(jié)果:1正常健康者與惡性腫瘤患者化療前兩組外周血單個核細胞功能之間比較:G0/G1期比例減低,S期比例升高,G2/M期比例升高,凋亡率升高,IL-8增高,差別均無統(tǒng)計學意義(P0.05)。2正常健康者與惡性腫瘤患者化療后兩組外周血單個核細胞功能之間比較:1)惡性腫瘤患者化療后較正常健康者G0/G1比例下降(86.06士9.08):(93.18士4.07),G2/M期比例升高(11.51士8.27):(4.00士2.24),差別有統(tǒng)計學意義(P0.05)。2)S期比例升高,IL-8比例增加,化療后較正常健康者外周血單個核細胞凋亡率增加,但差別均無統(tǒng)計學意義(P0.05)。3正常健康者與惡性腫瘤小負荷組化療后兩組之間外周血單個核細胞功能比較:1)化療后與正常健康者比較G0/G1比例下降(85.64士9.85):(93.18士4.07),G2/M期比例升高(12.11士8.45):(4.00士2.24),凋亡率增加(16.90士18.24):(2.90士1.87),差別有統(tǒng)計學意義(P0.05)。2)S期比例增加,IL-8比例增加,但差別無統(tǒng)計學意義(P0.05)。4正常健康者與惡性腫瘤大負荷組化療后兩組之間外周血單個核細胞功能比較。1)化療后與正常健康者比較G0/G1比例下降(86.62士8.33):(93.18士4.07),G2/M期比例升高(10.71士8.33):(4.00士2.24),差別有統(tǒng)計學意義(P0.05)。2)S期比例減低,IL-8比例增加,外周血單個核細胞凋亡率增加,但差別無統(tǒng)計學意義(P0.05)。5惡性腫瘤患者化療前后兩組之間外周血單個核細胞功能比較:1)化療后較化療前G0/G1比例下降,S期比例降低,G2/M期比例升高,IL-8比例減低,差別無統(tǒng)計學意義(P0.05)。2)化療后較化療前外周血單個核細胞凋亡率增加(12.63士18.09):(4.68士8.88),有統(tǒng)計學意義(P0.05)。6惡性腫瘤小負荷組患者化療前后兩組之間外周血單個核細胞功能比較。1)化療后較化療前G0/G1比例下降,S期比例下降,G2/M期比例升高,IL-8比例增加,但差別無統(tǒng)計學意義(P0.05)。2)化療后較化療前外周血單個核細胞凋亡率增加(16.90士18.24):(4.45士7.21),差別有統(tǒng)計學意義(P0.05)。7惡性腫瘤大負荷組患者化療前后兩組之間外周血單個核細胞功能比較。化療后較化療前G0/G1比例下降,S期比例增加,G2/M期比例升高,外周血單個核細胞凋亡率增加,IL-8比例減低,差別無統(tǒng)計學意義(P0.05)。8惡性腫瘤患者按照腫瘤負荷分亞組后兩組化療前外周血單個核細胞功能比較:化療前惡性腫瘤大負荷組較小負荷組IL-8比例增加(4.54±3.83):(2.06±2.14),差別有統(tǒng)計學意義(P0.05)。G0/G1比例增高,S期比例降低,G2/M期比例增高,凋亡率增加,均無統(tǒng)計學差異(P0.05)。9惡性腫瘤患者按照腫瘤負荷分亞組后兩組化療后外周血單個核細胞功能比較:化療后惡性腫瘤大負荷組較小負荷組G0/G1比例增加、S期比例減低、G2/M期比例減低、凋亡率減低及IL-8比例增加,均無統(tǒng)計學差異(P0.05)。結(jié)論:1惡性腫瘤化療前患者與正常健康者之間在細胞周期、凋亡率及IL-8水平均無統(tǒng)計學差異。2惡性腫瘤化療后患者與正常健康者之間在細胞周期上,較正常健康者G0/G1期比例減低,G2/M期比例升高。3化療干預后可增高外周血單個核細胞凋亡率,建議科研抽血時間為化療前。4 IL-8在化療前、腫瘤大負荷組明顯增高,表明IL-8更能反映惡性腫瘤患者外周血單個核細胞功能。5化療可明顯改變惡性腫瘤患者外周血單個核細胞細胞周期和凋亡率,為后續(xù)研究樣本抽血時間、免疫功能檢測提供理論依據(jù)。
[Abstract]:The occurrence of malignant tumor, development and proliferation of malignant tumor cells. The parasites in the human body, the body is in the environment for its survival. Therefore, the research of peripheral blood mononuclear cells change may provide theoretical basis for the further study of the relationship between malignant tumor cells and organism. Peripheral blood mononuclear cells, including lymphocytes and monocytes, lymphocytes including T lymphocytes, B lymphocytes and natural killer cells in immune surveillance, plays an extremely important role in killing target cells and immune regulation, is an important anti-tumor immune effector cells, the number and function of peripheral blood mononuclear cells of the body immune function level.IL-8 is a multicellular origin, a multi-functional cytokine, can promote inflammation, stimulate angiogenesis, promote mitosis, regulate host immune function and so on, in a variety of tumors High expression, progress and early events in tumorigenesis and tumor is closely related to the flow cytometry is a quantitative analysis and sorting of single cells or other biological particles in the function level detection means, is the detection of cell differentiation and function of the most advanced, the most accurate technique in the early diagnosis of cancer the main application of lesions and tumors, crossmatch, determination of bone marrow hematopoietic stem cell reconstruction, analysis of lymphocyte subsets, detection and analysis of cell apoptosis and differentiation of DNA cell cycle. Chemotherapy is the main treatment in malignant tumors, except has the function of killing tumor cells, also have damage to the body the normal tissue cells. Objective: To investigate the effect of chemotherapy for patients with malignant tumors of peripheral blood mononuclear cells. Methods: using flow cytometry in 43 cases of malignant tumor patients and 10 cases of normal health examination Morning fasting peripheral venous blood of peripheral blood mononuclear cell nuclear cell cycle, apoptosis rate and IL-8. Results: comparison between normal subjects and 1 patients with chemotherapy of malignant tumors in two groups of peripheral blood mononuclear cell function: the percentage of G0/G1 phase decreased, the percentage of S phase increased, the proportion of G2/M phase was increased, apoptosis the rate of increase of IL-8 increased, the difference was not statistically significant (P0.05) between the two groups of peripheral blood mononuclear cell function in patients with.2 chemotherapy normal and malignant tumor: 1) in patients with malignant tumors after chemotherapy compared with the normal G0/G1 ratio decreased (86.06 + 9.08): (93.18 + 4.07), G2/M increase the proportion of (11.51 + 8.27): (4 + 2.24), the difference was statistically significant (P0.05).2) S phase was increased, the proportion of IL-8 increased after chemotherapy than normal PBMC apoptosis rate increased, but there was no statistically significant difference (P0.05.3) and normal healthy people 鎭舵，

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