本文選題：肝細胞癌　切入點：甲基化譜　出處：《中國醫(yī)科大學學報》2017年12期

【摘要】：目的檢測人肝細胞癌細胞的DNA甲基化譜,明確肝細胞癌細胞中差異甲基化位點和基因的表達分布情況,進一步探討DNA異常甲基化與肝細胞癌發(fā)生發(fā)展的關系。方法使用DNA甲基化芯片(Infinium Human Methylation 450K Bead Chip)檢測人肝細胞癌細胞Huh7和人永生化肝細胞L02的甲基化譜,并對檢測結果進行生物學分析。結果共檢測到差異性甲基化位點102 254個,差異性甲基化基因26 511個,甲基化相關信號通路43個,其中57.3%的高甲基化CpG位點和39.4%的低甲基化CpG位點的甲基化差異程度≥50%,篩選后確定了3 222個顯著高甲基化基因和2 204個顯著低甲基化基因。結論在Huh7和L02細胞中存在大量差異性甲基化CpG位點及基因,Huh7細胞中可檢測到大量抑癌基因DNA的異常高甲基化,提示DNA異常甲基化與肝細胞癌的發(fā)生發(fā)展密切相關。
[Abstract]:Objective to detect the DNA methylation spectrum of human hepatocellular carcinoma cells and to determine the distribution of differentially methylated sites and genes in hepatocellular carcinoma cells. Methods DNA methylation microarray was used to detect the methylation spectrum of human hepatocellular carcinoma cell line Huh7 and human immortalized liver cell L02. The results showed that there were 102,254 differentially methylated sites, 26,511 differentially methylated genes and 43 methylation-related signaling pathways. Among them, 57.3% of the hypermethylated CpG sites and 39.4% of the hypomethylated CpG sites had significant methylation differences of 鈮，

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