本文選題：游離DNA　切入點：DNA完整性　出處：《腫瘤防治研究》2017年02期 　論文類型：期刊論文

【摘要】：目的監(jiān)測胃癌患者外周血游離DNA(cell-free DNA,cf-DNA)的含量,探討其在胃癌診治和預后中的價值。方法收集81例原發(fā)胃癌、18例胃良性腫瘤、19例胃癌前病變、43例同期門診健康體檢者以及部分術后和隨訪的外周血標本,提取游離DNA,熒光定量PCR檢測其含量(ALU115和ALU247)并計算完整性(ALU247/ALU115),分析血清游離DNA和血漿游離DNA含量之間的相關性。結果血漿游離DNA含量和完整性在四組之間的差異有統(tǒng)計學意義(ALU115、ALU247以及完整性的P值均0.001),術前術后的差異也有統(tǒng)計學意義(P值分別為0.000、0.013、0.010),隨訪前后完整性差異有統(tǒng)計學意義(P=0.029),在有無轉移、分期、分化程度間差異有統(tǒng)計學意義(P值分別為0.013、0.042、0.003)。血漿和血清游離DNA含量具有相關性且血漿離散趨勢更小。ALU247的ROC曲線下面積最高(0.985)。結論游離DNA檢測在胃癌的診治和預后中有一定的價值。
[Abstract]:The purpose of monitoring patients with gastric cancer peripheral blood free DNA (cell-free DNA, cf-DNA) content, and explore the diagnosis and treatment and prognosis of gastric carcinoma. Methods 81 cases of primary gastric carcinoma, 18 cases of gastric benign tumors, 19 cases of precancerous lesions, 43 cases of healthy year outpatient service and part of the postoperative and follow-up peripheral blood samples, extract free DNA, fluorescence quantitative PCR to detect the content of (ALU115 and ALU247) and integrity (ALU247/ALU115), analyze the correlation between the content of free DNA in serum free DNA and plasma. Results there were significant differences in plasma free DNA content and integrity between the four groups (ALU115, ALU247 and integrity the P value was 0.001), preoperative postoperative difference was statistically significant (P = 0.000,0.013,0.010), statistically significant difference before and after complete follow-up (P=0.029), in metastasis, staging, there was statistically significant difference between the degree of differentiation Meaning (P = 0.013,0.042,0.003). The free DNA content in plasma and serum is correlated and the plasma dispersion trend is smaller. The area under the ROC curve of.ALU247 is the highest (0.985). Conclusion free DNA detection has certain value in the diagnosis and prognosis of gastric cancer.

【作者單位】： 蘇州大學附屬第一醫(yī)院臨床檢測中心;
【基金】：2015年度蘇州市科技發(fā)展計劃(應用基礎研究-醫(yī)療衛(wèi)生)(SYSD2015078)
【分類號】：R735.2

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本文編號：1611618