本文關鍵詞：胚胎蛋白誘導多種腫瘤細胞凋亡的比較研究　出處：《東北師范大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 胚胎蛋白 MTT 腫瘤 凋亡 小鼠模型

【摘要】：腫瘤與細胞的異常分化直接相關。胚胎發(fā)育,是細胞不斷增殖、誘導正常細胞分化、并伴有凋亡的過程,而在這其間出現(xiàn)的異常細胞分化則會被誘導修正。胚胎在發(fā)育過程中能夠產生促分化因子,誘導胚胎細胞分化、凋亡,決定胚胎發(fā)育的方向。近年來促分化因了的應用,尤其在誘導腫瘤細胞凋亡方面,引起了人們的廣泛關注。經本實驗室前期研究發(fā)現(xiàn),動物的胚胎蛋白在誘導腫瘤細胞凋亡方面作用顯著,并且具有普遍性。本文進一步驗證,胚胎提取蛋白對于多種腫瘤細胞的體外培養(yǎng)過程中,均具有抑制細胞增殖、誘導凋亡的作用,并初步驗證胚胎蛋白對小鼠體內腫瘤細胞同樣具有誘導凋亡的作用。本文選用雞胚胎提取蛋白以及蛙胚胎提取蛋白作為誘導物,以不同濃度梯度分別誘導多同腫瘤細胞,以比對其誘導凋亡差異性。選用的腫瘤細胞主要有：RM1、HepG2、NLT、MCF7、Hela、B16等。通過光學顯微鏡、電子顯微鏡、激光共聚焦顯微鏡,以及MTT,流式細胞儀等檢測,證實胚胎蛋白對各種腫瘤細胞均具有抑制增殖、誘導凋亡的效果,且具有誘導物濃度影響誘導效果的差異。將B16細胞接種到小鼠皮下,建立體內腫瘤動物模型,采用原位注射法,將胚胎蛋白注入小鼠體內,結果證實,在實驗組小鼠體內,腫瘤的體積,與對照組相比明顯減小。初步驗證了胚胎蛋白對生長在小鼠體內的腫瘤細胞同樣具有誘導凋亡的作用。
[Abstract]:Tumor is directly related to abnormal differentiation of cells. Embryonic development is the process of cell proliferation, induction of normal cell differentiation and apoptosis. However, the abnormal cell differentiation will be induced and corrected during the process of embryo development, which can induce differentiation and apoptosis of embryonic cells. In recent years, the application of differentiation promotion, especially in inducing apoptosis of tumor cells, has attracted wide attention. Animal embryonic proteins play a significant role in inducing apoptosis of tumor cells, and they are universal. In this paper, we further verify that embryonic proteins can be used in the culture of various tumor cells in vitro. Both of them can inhibit cell proliferation and induce apoptosis. It was also proved that embryonic protein could induce apoptosis in mouse tumor cells. Chicken embryo extract protein and frog embryo extract protein were selected as inducers. Different concentrations of gradient were used to induce the same tumor cells, and to compare the difference of inducing apoptosis. The main tumor cells selected were: RM1, HepG2, NLTG2NLTMCF7 and Hela. B16 et al. By optical microscope, electron microscope, laser confocal microscope, MTT, flow cytometry and other detection, it was proved that embryonic protein can inhibit the proliferation of various tumor cells. B16 cells were inoculated subcutaneously into mice to establish tumor animal model in vivo, and in situ injection method was used. Embryonic proteins were injected into mice and the results showed that the tumor volume was measured in mice of experimental group. Compared with the control group, it was proved that embryonic protein could induce apoptosis of tumor cells in mice.
【學位授予單位】：東北師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R73-36

【參考文獻】

相關期刊論文 前4條

1 黃寧;彭芳;;抗腫瘤藥物動物模型的評價[J];大理學院學報;2008年02期

2 楊聯(lián)云;趙世巧;;腫瘤免疫治療的研究進展[J];檢驗醫(yī)學與臨床;2007年05期

3 楊吉成;2002年世界十大科學成就之首——siRNA和RNAi[J];蘇州大學學報(醫(yī)學版);2003年03期

4 敖敏;何剛;梁傳余;;重組人p53腺病毒藥物對人喉癌細胞的抑制實驗[J];現(xiàn)代預防醫(yī)學;2007年06期

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本文編號：1356013