【摘要】：目的探討海水淹溺型急性肺損傷(SWD-ALI)時外周血中性粒細胞(PMN)凋亡的演變規(guī)律以及細胞因子對其可能的影響。方法36只新西蘭兔用隨機數(shù)字表法均分為對照組(作為0h基線值)和海水灌注后1、3、6、12、24h組(分別于灌注后1、3、6、12、24h處死)。觀察各組血象和血氣分析指標(biāo)的變化,計算肺濕/干重比(W/D)和肺微血管通透指數(shù);流式細胞術(shù)檢測PMN凋亡情況,ELISA法檢測血清TNF-α、IL-1β、IL-10水平;對肺組織進行病理學(xué)觀察,并計算病理評分(LPS)。結(jié)果W/D值在3h達高峰,肺微血管通透指數(shù)以6h最高,炎癥細胞浸潤以6～12h最顯著。LPS在1h已顯著高于對照組(P0.01),6h達最高。PMN凋亡率于灌注后1h一過性升高,3h顯著抑制,6h最低,以后逐漸接近對照水平。PMN計數(shù)與凋亡率呈負相關(guān)(P0.05)。各海水灌注組血清TNF-α、IL-1β、IL-10均顯著高于對照組(P0.05或P0.01),與LPS呈正相關(guān)(P0.05或P0.01),但與PMN凋亡率無相關(guān)性(P0.05)。結(jié)論PMN凋亡在SWD-ALI炎癥反應(yīng)早期受到顯著抑制,是最主要的炎癥效應(yīng)細胞。TNF-α、IL-1β、IL-10在SWD-ALI中發(fā)揮重要作用,但對PMN凋亡抑制無顯著影響。
[Abstract]:Objective to investigate the evolution of (PMN) apoptosis in peripheral blood neutrophils during seawater drowning acute lung injury (SWD-ALI) and the possible effect of cytokines on it. Methods 36 New Zealand rabbits were randomly divided into control group (as 0 h baseline value) and 1, 3, 6, 12, 24 h group (1, 3, 6, 12, 24 h after seawater perfusion, respectively). The changes of hemogram and blood gas analysis indexes were observed, the lung wet / dry weight ratio (W 鈮，

本文編號：2481934