【摘要】：目的探索模擬失重下空間誘變大腸桿菌(T1-13)感染巨噬細(xì)胞RAW264.7后差異表達(dá)的microRNAs(miRNAs)及其意義。方法采用第二代測(cè)序技術(shù)檢測(cè)模擬失重染菌組與對(duì)照組巨噬細(xì)胞miRNAs差異表達(dá),通過生物信息學(xué)預(yù)測(cè)差異miRNAs的靶基因,并對(duì)靶基因進(jìn)行GO功能富集分析、KEGG信號(hào)通路分析以及STRING蛋白互作分析。結(jié)果模擬失重染菌組與對(duì)照組間篩選出33個(gè)差異miRNAs,下調(diào)23個(gè),上調(diào)10個(gè)。這些miRNAs互補(bǔ)結(jié)合的靶基因參與了MAPK通路、WNT通路、軸突導(dǎo)向以及TGF-β等信號(hào)通路,其中關(guān)鍵下調(diào)的靶基因是MAPK9,MAPK14,MAP3K7,MAP3K14,PIK3CA,PIK3CB,PIK3CD,MYC,JUN,SMAD4;關(guān)鍵上調(diào)的靶基因是AKT3,MAPK8,MAPK9,MAPK10,MAP2K1,PIK3CD。結(jié)論模擬失重下空間誘變大腸桿菌感染巨噬細(xì)胞后miRNAs表達(dá)出現(xiàn)顯著差異,這些miRNAs可能通過調(diào)控MAPK,WNT以及TGF-β等通路參與失重條件下炎癥反應(yīng)的發(fā)生。
[Abstract]:Objective to explore the differential expression of microRNAs (miRNAs) in macrophages infected with space mutated Escherichia coli (T1-13) under simulated weightlessness and its significance. Methods the second generation sequencing technique was used to detect the differential expression of miRNAs in macrophages of simulated weightlessness group and control group. The target genes of differential miRNAs were predicted by bioinformatics, and the GO function enrichment analysis was carried out on the target genes. KEGG signal pathway analysis and STRING protein interaction analysis. Results 33 differences in miRNAs, were down-regulated by 23 and increased by 10 between the simulated weightlessness group and the control group. These complementary miRNAs binding target genes are involved in MAPK pathway, WNT pathway, axon orientation and TGF- 尾 signaling pathway. The key down-regulated target gene is MAPK9,MAPK14,MAP3K7,MAP3K14,PIK3CA,PIK3CB,PIK3CD,MYC,JUN,SMAD4;. The key up-regulated target gene is AKT3,MAPK8,MAPK9,MAPK10,MAP2K1,PIK3CD. Conclusion there is a significant difference in miRNAs expression in macrophages infected by space mutagenesis under simulated weightlessness. These miRNAs may be involved in inflammatory reaction under weightlessness through regulating MAPK,WNT and TGF- 尾 pathways.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院;石河子大學(xué)第一附屬醫(yī)院檢驗(yàn)科;錦州醫(yī)科大學(xué);中國(guó)航天員科研訓(xùn)練中心航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國(guó)家重點(diǎn)實(shí)驗(yàn)室;解放軍總醫(yī)院南樓呼吸科;
【基金】：國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(2014CB744404)
【分類號(hào)】：R852.22

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