本文選題：晶狀體 + 兔��； 參考：《天津醫(yī)科大學》2006年碩士論文

【摘要】：目的:觀察將海水注入前房對兔眼晶狀體功能及形態(tài)學的影響,并對其致傷機制進行探討。 方法:隨機選擇55只兔子中的50只建立動物模型,右眼前房注入0.2ml海水,分別于術后15分鐘、30分鐘、1小時、3小時、6小時及12小時經(jīng)裂隙燈顯微鏡檢查后摘除眼球行光電鏡檢查及Na~+,K~+-ATP酶、Ca~(2+),Mg~(2+)-ATP酶、琥珀酸脫氫酶(Succinate dehydrogenase,SDH)、乳酸脫氫酶(Lactate dehydrogenase,LDH)、超氧化物歧化酶(Superoxide Dismutase,SOD)和谷光甘肽過氧化物酶(Glutathione-Peroxide,GSH-PX)的生化活性檢測。左眼前房內(nèi)注入0.2ml平衡鹽溶液(Balanced salt solution,BSS)作為自身對照,其余步驟同右眼,另外5只兔子不做任何處理為正常組。 結果:1、裂隙燈顯微鏡觀察海水組兔子晶狀體起初明顯混濁,6小時后混濁晶狀體逐漸恢復透明。2、光鏡下海水組細胞表現(xiàn)為水腫、變性、壞死及Morgagnian小體形成,對照組細胞輕度水腫,無明顯Morgagnian小體形成。3、電鏡下海水組細胞逐漸出現(xiàn)線粒體、粗面內(nèi)質(zhì)網(wǎng)和高爾基復合體水腫擴張及空泡化改變,對照組細胞內(nèi)超微結構輕度水腫。4、海水組品狀體Na~+,K~+-ATPase及Ca~(2+),Mg~(2+)-ATPase活性均先降低,再增高,最后完全或部分恢復正常;SDH及LDH活性6小時內(nèi)均代償性增高,12小時恢復正常;SOD
[Abstract]:Aim: to observe the effects of seawater injection into anterior chamber on lens function and morphology of rabbit eyes, and to explore the mechanism of injury. Methods: fifty of 55 rabbits were randomly selected to establish animal models, and 0.2ml seawater was injected into the right anterior chamber. After 15 minutes, 30 minutes, 1 hour, 3 hours, 6 hours and 12 hours, the eyeballs were removed by slit lamp microscope and examined with photoelectricity microscope and Na ~ + K ~ -ATPase. The biochemical activities of succinate dehydrogenase (Succinate dehydrogenase), lactate dehydrogenase (Lactate dehydrogenase), superoxide dismutase (SOD) and glutathione-peroxidase (Glutathione-Peroxide-GSH-PX) were detected. 0.2ml balanced salt solution was injected into the left anterior chamber as self-control, the other steps were the same as the right eye, and the other 5 rabbits were not treated as normal group. Results under the slit lamp microscope, the lens of rabbits in seawater group recovered gradually to hyaline 0.2 after 6 hours of obvious opacification. The cells of seawater group showed edema, degeneration, necrosis and formation of Morgagnian bodies under light microscope, while those in control group showed mild edema. There was no obvious formation of Morgagnian corpuscles. Under electron microscope, mitochondria, rough endoplasmic reticulum and Golgi complex were gradually appeared in the cells of seawater group. In the control group, the ultrastructure of the cells was slightly edematous .4.The activities of Na ~ + K ~ -ATPase and Ca~(2 _ 2 ~ -ATPase decreased first, then increased, and finally returned to normal completely or partially within 6 hours, and then returned to normal in 12 hours.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R82

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