本文選題：腹部損傷　切入點(diǎn)：開放性　出處：《第二軍醫(yī)大學(xué)》2005年碩士論文

【摘要】：目的 探討腹部開放傷合并海水浸泡中內(nèi)毒素及脂多糖結(jié)合蛋白(lipopolysaccharide-binding protein,LBP)和CD14的作用。方法 雄性大鼠60只,隨機(jī)分為正常組、腹部開放傷對(duì)照組、腹部開放傷合并海水浸泡0.5h、1h、2h、3h組(n=10)。檢測血漿中的內(nèi)毒素水平和肝、肺中LBP及CD14蛋白,并進(jìn)行病理觀察。另取雄性大鼠36只,隨機(jī)分為6組(n=6),檢測肝、肺中LBP、CD14及TLR4的mRNA。結(jié)果 腹部開放傷合并海水浸泡組各臟器病理變化較重。受傷大鼠經(jīng)海水浸泡2h后,血漿中內(nèi)毒素水平明顯升高。大鼠肝及肺中LBP及CD14mRNA在浸泡2h后顯著升高,而TLR4 mRNA則未見明顯變化。肝及肺的LBP、CD14蛋白表達(dá)也有類似的趨勢。結(jié)論 腹部開放傷合并海水浸泡后,內(nèi)毒素血癥上調(diào)肝及肺中的LBP、CD14,內(nèi)毒素-LBP/CD14-炎癥介質(zhì)之間形成“正反饋環(huán)”,放大炎癥反應(yīng),加重?fù)p傷。
[Abstract]:Objective to investigate the effect of endotoxin lipopolysaccharide and open abdominal wound with seawater immersion binding protein (lipopolysaccharide-binding protein, LBP) and CD14. Methods: male 60 rats were randomly divided into normal group, open abdominal injury group, 0.5h, open abdominal injury combined with seawater immersion of 1H, 2h, 3h group (n=10) detection of plasma. The level of endotoxin and liver, LBP and CD14 protein in the lung and pathological observation. Another 36 male rats were randomly divided into 6 groups (n=6), the detection of liver, lung LBP, CD14 and TLR4 mRNA. results of the open abdominal injury and seawater immersion group, pathological changes were severe. The injured rats with seawater immersion after 2h, the plasma levels of endotoxin in rats increased significantly. LBP and CD14mRNA in liver and lung was significantly higher in 2H and TLR4 after soaking, mRNA had no obvious change. The liver and lung LBP, the protein expression of CD14 had a similar trend. Conclusion open abdominal injury After seawater immersion, endotoxemia up-regulated LBP and CD14 in liver and lung, and positive and negative feedback loops between endotoxin -LBP/CD14- inflammatory mediators, amplifying inflammatory reaction and aggravating injury.

【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R826

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本文編號(hào)：1719752