酪氨酸羥化酶基因SNP多態(tài)性與運(yùn)動性失眠的實(shí)驗(yàn)研究
發(fā)布時(shí)間：2018-10-10 13:32
【摘要】：運(yùn)動性失眠是失眠癥的一種特殊類型，是運(yùn)動性疲勞的癥狀之一。適度的運(yùn)動有助于睡眠，但是超過一定的限度就有可能導(dǎo)致失眠即運(yùn)動性失眠。關(guān)于失眠是否存在一定的遺傳性，，曾有研究人員通過調(diào)查并證實(shí)了失眠的遺傳性，兒茶酚胺是人體內(nèi)重要激素及神經(jīng)遞質(zhì)，由酪氨酸合成而來，合成過程中有四種酶參與，酪氨酸羥化酶(tyrosine hydroxylase)是第一個(gè)酶，催化酪氨酸生成多巴，在調(diào)節(jié)中樞神經(jīng)和交感神經(jīng)系統(tǒng)功能上發(fā)揮著重要的作用，由于TH在調(diào)節(jié)中樞神經(jīng)中的重要作用，本文預(yù)從TH基因水平來探討運(yùn)動性失眠發(fā)生。 目的：通過問卷調(diào)查、PCR技術(shù)測序，分析酪氨酸羥化酶基因SNP基因分型在失眠組與非失眠組統(tǒng)計(jì)學(xué)差異，探討酪氨酸羥化酶基因多態(tài)性與運(yùn)動性失眠發(fā)生是否存在一定的相關(guān)性。 方法：用問卷調(diào)查法對成都體育學(xué)院體育系運(yùn)動系的籃球班、武術(shù)班、健美操班、舉重班、田徑班、籃球隊(duì)、武術(shù)隊(duì)、健美操隊(duì)、舉重隊(duì)、田徑隊(duì)430名運(yùn)動員進(jìn)行主觀感覺調(diào)查，按照運(yùn)動性失眠的評定標(biāo)準(zhǔn)進(jìn)行分析判斷，把受試者分成失眠組與非失眠組，應(yīng)用聚合酶鏈反應(yīng)一限制性片段長度多態(tài)性(PcR-RFLP)的分析方法，通過測序得到失眠組16人的酪氨酸羥化酶基因序列和非失眠組18人的酪氨酸羥化酶基因序列。通過比較失眠組與非失眠組酪氨酸羥化酶基因SNP多態(tài)性，來判斷酪氨酸羥化酶基因是否與運(yùn)動性失眠的發(fā)生存在一定的聯(lián)系。 結(jié)果：1、運(yùn)動員疲勞自覺癥狀測定，其中67%的運(yùn)動員得分處于第四個(gè)等級（19分-24分）。2、籃球、武術(shù)、健美操、舉重、田徑5個(gè)項(xiàng)目的運(yùn)動員運(yùn)動訓(xùn)練后舉重運(yùn)動員100%出現(xiàn)形體疲勞，20%出現(xiàn)臟腑疲勞，9%出現(xiàn)神志疲勞；籃球、武術(shù)、健美操、田徑出現(xiàn)神志疲勞的概率分別為：60%、52%、50%、36%。3、從實(shí)驗(yàn)數(shù)據(jù)分析酪氨酸羥化酶基因位點(diǎn)G-494A、C-388T、A-641G的分型在運(yùn)動性失眠組與非失眠組存在統(tǒng)計(jì)學(xué)差異，而G-94T、A-581G、T-741C、G-801C、G-814A、C-824T和C-833T等7個(gè)位點(diǎn)統(tǒng)計(jì)結(jié)果無統(tǒng)計(jì)學(xué)差異。 結(jié)論：1、運(yùn)動訓(xùn)練后67%的運(yùn)動員會出現(xiàn)中高等程度的疲勞。2、舉重運(yùn)動員出現(xiàn)形體疲勞比率較大，出現(xiàn)臟腑疲勞和神志疲勞的比率較小。籃球、武術(shù)、健美操、田徑項(xiàng)目的運(yùn)動員出現(xiàn)臟腑疲勞和神志疲勞的比率較大。3、酪氨酸羥化酶基因位點(diǎn)G-494A、C-388T、A-641G的多態(tài)性與運(yùn)動性失眠的發(fā)生存在一定的相關(guān)跡象，但是由于采集的樣本量大少還不能證明酪氨酸羥化酶基因位點(diǎn)G-494A、C-388T、A-641G與運(yùn)動性失眠存在確切相關(guān),同時(shí)酪氨酸羥化酶基因G-94T、A-581G、T-741C、G-801C、G-814A、C-824T和C-833T等7個(gè)位點(diǎn)在本實(shí)驗(yàn)結(jié)果中沒有出現(xiàn)與運(yùn)動性失眠相關(guān)的跡象。
[Abstract]:Exercise insomnia is a special type of insomnia and one of the symptoms of exercise fatigue. Moderate exercise helps sleep, but beyond a certain limit can lead to insomnia, or exercise insomnia. As to whether insomnia is hereditary or not, some researchers have investigated and confirmed the heredity of insomnia. Catecholamine is an important hormone and neurotransmitter in the human body, which is synthesized by tyrosine and involved in the synthesis of four enzymes. Tyrosine hydroxylase (tyrosine hydroxylase) is the first enzyme to catalyze tyrosine to produce dopa, which plays an important role in regulating the function of central nervous system and sympathetic nervous system, because of the important role of TH in regulating central nervous system. In this study, TH gene level was used to study the occurrence of motor insomnia. Objective: to analyze the statistical difference of tyrosine hydroxylase gene SNP genotyping between insomnia group and non-insomnia group by questionnaire investigation and PCR sequencing, and to explore whether there is a certain correlation between tyrosine hydroxylase gene polymorphism and motor insomnia. Methods: the basketball class, martial arts class, aerobics class, weightlifting class, track and field class, basketball team, martial arts team, calisthenics team, weightlifting team, and weightlifting team were investigated by questionnaire in Department of physical Education of Chengdu Institute of physical Education. Three hundred and thirty athletes of track and field team investigated their subjective feelings, analyzed and judged according to the evaluation criteria of motor insomnia, divided them into insomnia group and non-insomnia group, and used polymerase chain reaction-restriction fragment length polymorphism (PcR-RFLP) analysis method. The tyrosine hydroxylase gene sequences of 16 patients with insomnia and 18 patients with no insomnia were obtained by sequencing. By comparing the SNP polymorphism of tyrosine hydroxylase gene between insomnia group and non-insomnia group, the relationship between tyrosine hydroxylase gene and motor insomnia was determined. Results: 1, the athletes' fatigue conscious symptoms were measured, of which 67% of the athletes scored in the fourth grade (19-24 points). 2, basketball, martial arts, aerobics, weightlifting, 100% of weightlifters developed physical fatigue, 20% viscera fatigue, 9% mental fatigue, basketball, martial arts, calisthenics, The probability of mental fatigue in track and field were as follows: 60: 52and 50: 360.The tyrosine hydroxylase gene locus G-494AnC-388TFA-641G was analyzed from the experimental data. There was statistical difference between the exercise insomnia group and the non-insomnia group, but there was no statistical difference between the G-94 TA- 581GT-741GT-801C741 G-814AHN C-824T and C-833T loci. Conclusion: 1. After training, 67% of athletes will develop middle and higher degree fatigue. 2. Weight lifters have higher body fatigue rate, and lower ratio of viscera fatigue and mental fatigue. Athletes in basketball, martial arts, aerobics and track and field events have higher rates of viscera fatigue and mental fatigue. 3. The polymorphism of tyrosine hydroxylase gene locus G-494AnC-388TKA-641G is associated with the occurrence of sports insomnia. However, because the sample size was large and small, the tyrosine hydroxylase gene locus G-494An C-388TKA-641G could not be proved to be related to motor insomnia. At the same time, 7 loci of tyrosine hydroxylase gene G-94 TX A-581GFT-741CU G-801CU G-814ANC-824T and C-833T did not show any correlation with motor insomnia in this study.
【學(xué)位授予單位】：成都體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R87
本文編號：2261974
[Abstract]:Exercise insomnia is a special type of insomnia and one of the symptoms of exercise fatigue. Moderate exercise helps sleep, but beyond a certain limit can lead to insomnia, or exercise insomnia. As to whether insomnia is hereditary or not, some researchers have investigated and confirmed the heredity of insomnia. Catecholamine is an important hormone and neurotransmitter in the human body, which is synthesized by tyrosine and involved in the synthesis of four enzymes. Tyrosine hydroxylase (tyrosine hydroxylase) is the first enzyme to catalyze tyrosine to produce dopa, which plays an important role in regulating the function of central nervous system and sympathetic nervous system, because of the important role of TH in regulating central nervous system. In this study, TH gene level was used to study the occurrence of motor insomnia. Objective: to analyze the statistical difference of tyrosine hydroxylase gene SNP genotyping between insomnia group and non-insomnia group by questionnaire investigation and PCR sequencing, and to explore whether there is a certain correlation between tyrosine hydroxylase gene polymorphism and motor insomnia. Methods: the basketball class, martial arts class, aerobics class, weightlifting class, track and field class, basketball team, martial arts team, calisthenics team, weightlifting team, and weightlifting team were investigated by questionnaire in Department of physical Education of Chengdu Institute of physical Education. Three hundred and thirty athletes of track and field team investigated their subjective feelings, analyzed and judged according to the evaluation criteria of motor insomnia, divided them into insomnia group and non-insomnia group, and used polymerase chain reaction-restriction fragment length polymorphism (PcR-RFLP) analysis method. The tyrosine hydroxylase gene sequences of 16 patients with insomnia and 18 patients with no insomnia were obtained by sequencing. By comparing the SNP polymorphism of tyrosine hydroxylase gene between insomnia group and non-insomnia group, the relationship between tyrosine hydroxylase gene and motor insomnia was determined. Results: 1, the athletes' fatigue conscious symptoms were measured, of which 67% of the athletes scored in the fourth grade (19-24 points). 2, basketball, martial arts, aerobics, weightlifting, 100% of weightlifters developed physical fatigue, 20% viscera fatigue, 9% mental fatigue, basketball, martial arts, calisthenics, The probability of mental fatigue in track and field were as follows: 60: 52and 50: 360.The tyrosine hydroxylase gene locus G-494AnC-388TFA-641G was analyzed from the experimental data. There was statistical difference between the exercise insomnia group and the non-insomnia group, but there was no statistical difference between the G-94 TA- 581GT-741GT-801C741 G-814AHN C-824T and C-833T loci. Conclusion: 1. After training, 67% of athletes will develop middle and higher degree fatigue. 2. Weight lifters have higher body fatigue rate, and lower ratio of viscera fatigue and mental fatigue. Athletes in basketball, martial arts, aerobics and track and field events have higher rates of viscera fatigue and mental fatigue. 3. The polymorphism of tyrosine hydroxylase gene locus G-494AnC-388TKA-641G is associated with the occurrence of sports insomnia. However, because the sample size was large and small, the tyrosine hydroxylase gene locus G-494An C-388TKA-641G could not be proved to be related to motor insomnia. At the same time, 7 loci of tyrosine hydroxylase gene G-94 TX A-581GFT-741CU G-801CU G-814ANC-824T and C-833T did not show any correlation with motor insomnia in this study.
【學(xué)位授予單位】：成都體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R87
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本文編號：2261974
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