本文選題：髕骨 + 運動醫(yī)學��； 參考：《中國組織工程研究》2017年12期

【摘要】：背景:髕骨髕腱結(jié)合部損傷在運動訓練中十分常見且復發(fā)率較高,及時合理的恢復訓練對保持競技狀態(tài)有利,而不恰當?shù)幕謴陀柧殑t有可能加重損傷。目的:從組織形態(tài)學、生長因子、膠原蛋白表達量及肌張力等角度,探討傷后休息不同天數(shù)恢復訓練對骨腱結(jié)合部愈合質(zhì)量的影響。方法:40只新西蘭大白兔被隨機分為:空白對照組,針刺后分別休息24,48,72,96,120 h開始恢復訓練組。除空白對照組外,其余各組于髕腱止點處進行針刺損傷造模后采用已建立的循環(huán)載荷訓練模型,進行4周低強度訓練后處死取材,行組織學蘇木精-伊紅、番紅及免疫組織化學染色;每次訓練后利用Myoton-3肌肉功能分析儀采集肌張力,肌肉硬度數(shù)據(jù);Metamorph圖形處理軟件對各組纖維軟骨帶厚度、細胞密度,血管內(nèi)皮生長因子、膠原陽性染色細胞密度進行定量計算。結(jié)果與結(jié)論:(1)與空白對照組相比,各傷后訓練組在呈現(xiàn)出不同程度的組織形態(tài)改變,包括膠原排列紊亂,細胞密度降低,黏多糖蛋白增多,出現(xiàn)"漲潮"現(xiàn)象等;(2)針刺后24 h與針刺后120 h組纖維軟骨帶厚度比空白對照組顯著增厚(P0.05);(3)針刺后24 h組與針刺后48 h組的Ⅰ型膠原與Ⅲ型膠原比例顯著低于空白對照組(P0.05),72 h組最高;(4)針刺后48 h,針刺后72 h與針刺后96 h組的血管內(nèi)皮生長因子表達量相較于空白對照組顯著增高(P0.05);(5)肌張力與膠原比例(r=-0.538;P0.01)及軟骨細胞密度(r=-0.570;P0.01)呈負相關(guān)關(guān)系,而肌肉硬度則與血管內(nèi)皮生長因子表達量呈正相關(guān)關(guān)系(r=0.613;P0.01);(6)結(jié)果說明,急性損傷后不同開始恢復訓練時間會對髕骨髕腱結(jié)合部的愈合造成明顯的差異,傷后休息72 h開始訓練是最為合理的選擇;研究證實了肌張力及肌肉硬度會對細胞密度,膠原合成及生長因子表達存在相關(guān)性,此指標測量方便且無創(chuàng),可應用在傷后訓練及預防損傷復發(fā)等方面,指導運動員及普通人科學運動。
[Abstract]:Background: patellar tendon injury is very common in sports training and the recurrence rate is high. Timely and reasonable recovery training is beneficial to maintain competitive state, but improper recovery training may aggravate the injury. Objective: to investigate the effect of different days of rest training on the healing quality of tendon joint from the angles of histomorphology, growth factor, collagen expression and muscle tension. Methods 40 New Zealand white rabbits were randomly divided into two groups: the blank control group. With the exception of the blank control group, the other groups were treated with acupuncture injury at the point of patellar tendon insertion, and then the established cyclic load training model was used. After 4 weeks of low-intensity training, the animals were killed to get the material, and histopathological hematoxylin eosin was used. After each training, myoton-3 muscle function analyzer was used to collect muscle tension, muscle hardness data and Metamorph graphic processing software for the thickness of fibrochondrocytes, cell density and vascular endothelial growth factor (VEGF) in each group. The cell density of collagen positive staining was calculated quantitatively. Results and conclusion: (1) compared with the blank control group, the training groups showed different degrees of tissue morphological changes, including the disorder of collagen arrangement, the decrease of cell density, the increase of mucopolysaccharide protein. (2) the thickness of fibrous chondrocytes in the groups of 24 h after acupuncture and 120h after acupuncture was significantly thicker than that in the blank control group (P0.05); (3) the ratio of type 鈪，

本文編號：2104999