本文選題：法醫(yī)物證學 + 等位基因分型標準物　； 參考：《山西醫(yī)科大學》2013年碩士論文

【摘要】：目的 調查D11S4463基因座和D17S974基因座在中國漢族人群中的遺傳多態(tài)性,并用分子克隆技術制備其等位基因分型標準物。 方法 用熒光PCR和ABI3130XL遺傳分析儀對中國漢族600份無關個體血斑樣本進行D11S4463和D17S974基因座遺傳多態(tài)性調查,并用分子克隆的方法構建其等位基因分型標準物。首先在人群中分別篩選出D11S4463基因座的9個等位基因和D17S974基因座的8個等位基因,經PCR擴增,并將純化后的擴增產物與pMD18-T質粒載體連接后轉化到DH5α感受態(tài)大腸桿菌中；篩選重組子及提取重組質粒,對等位基因重組質粒進行序列測定,并對等位基因進行標準命名；以等位基因重組質粒為模板制備等位基因分型標準物。 結果 D11S4463基因座和D17S974基因座在中國漢族人群中具有較高的遺傳多態(tài)性,分別檢測出9個和8個等位基因,其觀察雜合度分別為0.735和0.747；匹配概率分別為0.089和0.115；個體識別能力分別為0.911和0.885；多態(tài)性信息含量分別為0.73和0.70；非父排除概率分別為0.485和0.505。應用分子克隆方法成功構建了D11S4463基因座和D17S974基因座等位基因分型標準物。 結論 D11S4463基因座和D17S974基因座具有較高的遺傳多態(tài)性,是一個適合于法醫(yī)學和群體遺傳學研究的遺傳標記,應用分子克隆的方法構建其等位基因分型標準物是一種實現(xiàn)其等位基因分型標準化的可行辦法。
[Abstract]:Objective to investigate the genetic polymorphism of D11S4463 locus and D17S974 locus in Chinese Han population and to prepare their alleles by molecular cloning. Methods the genetic polymorphisms of D11S4463 and D17S974 loci were investigated by fluorescence PCR and ABI3130XL genetic analyzer in 600 unrelated individuals of Han nationality in China, and their alleles were constructed by molecular cloning. At first, 9 alleles of D11S4463 locus and 8 alleles of D17S974 locus were screened out in the population. The purified products were amplified by PCR and transformed into DH5 偽 competent Escherichia coli by ligation with pMD18-T plasmid vector. The allelic recombinant plasmids were sequenced and the alleles were named as standard alleles, and the allelic genotyping standard compounds were prepared by using allelic recombinant plasmids as template. Results D11S4463 locus and D17S974 locus were highly polymorphic in Chinese Han population, 9 and 8 alleles were detected respectively, the observed heterozygosity were 0.735 and 0.747, the matching probability were 0.089 and 0.115, respectively. The recognition ability of individuals was 0.911 and 0.885, the polymorphic information content was 0.73 and 0.70, and the non-paternal exclusion probability was 0.485 and 0.505, respectively. The alleles of D11S4463 locus and D17S974 locus were successfully constructed by molecular cloning. Conclusion D11S4463 locus and D17S974 locus are highly polymorphic, which is a suitable genetic marker for forensic and population genetics research. It is a feasible method to construct its allelic genotyping standard by molecular cloning.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：D919.4

【參考文獻】

相關期刊論文 前10條

1 袁麗;魯滌;石美森;楊雪;;岫巖滿族9個STR基因座遺傳多態(tài)性[J];證據(jù)科學;2011年05期

2 張林,辛軍平,廖淼,陳國弟,李榮華,吳梅筠;用分子克隆技術構建D12S375基因座等位基因分型標準物及漢、回、維族群體遺傳學研究[J];中國法醫(yī)學雜志;2001年04期

3 陳慧,余純應,楊慶恩,楊榮芝,梅q,徐雯;STR等位基因梯階制備的研究[J];中國法醫(yī)學雜志;2002年04期

4 胡慧英;劉超;楊電;劉長暉;;兩無關個體單親親子鑒定不排除1例[J];中國法醫(yī)學雜志;2006年05期

5 陳玲;侯慶唐;王冬花;;三聯(lián)體親子鑒定1例[J];中國法醫(yī)學雜志;2008年01期

6 巴華杰;羅斌;劉冰泉;林子清;馬駿;朱愛華;;DNA數(shù)據(jù)庫9個STR基因座比中認定親權的可靠性分析[J];中國法醫(yī)學雜志;2008年04期

7 白雪;叢斌;李淑瑾;谷建立;侯志平;劉寧;李霞;;河北漢族群體D17S974和D11S4463 miniSTR遺傳多態(tài)性[J];中國法醫(yī)學雜志;2009年03期

8 邵偉波;張素華;李莉;;21個非CODIS STR基因座的遺傳多態(tài)性[J];法醫(yī)學雜志;2011年01期

9 李生斌,鄭海波,馮繼東,于嘉琳;中華民族STR遺傳結構及變化規(guī)律的研究(Ⅰ)[J];西安醫(yī)科大學學報(中文版);2000年01期

10 賴江華,張保華,朱波峰,陳騰,鄭海波,尋兮,李生斌;云南白族STR遺傳多態(tài)性研究[J];西安交通大學學報(醫(yī)學版);2002年03期

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