本文選題：有氧運(yùn)動 + 腦衰老�。� 參考：《成都體育學(xué)院》2017年碩士論文

【摘要】：目的:SD雄性大鼠腹腔注射D-半乳糖制作衰老模型,在大鼠造模前、造模過程中、造模后進(jìn)行有氧運(yùn)動(游泳,60min/d)干預(yù),檢測大鼠腦衰老指標(biāo)、空間學(xué)習(xí)記憶能力、小腦腦源性神經(jīng)營養(yǎng)因子BDNF的表達(dá),探討有氧運(yùn)動對大鼠腦衰老、學(xué)習(xí)記憶能力及小腦BDNF的影響。方法:將SD大鼠(72只,3月齡,雄性)按照SD(造模前有氧運(yùn)動預(yù)處理組)、ND(造模組)、NNa(注射生理鹽水對照組)、NDs(造模過程中有氧運(yùn)動組)、NDS(造模后有氧運(yùn)動組)及NDN(造模后自然喂養(yǎng)組)隨機(jī)分為6個大組。在造模結(jié)束后每個大組隨機(jī)分為2小組:M組(SDM組、NDM組、NNa M組、NDs M組、NDSM組、NDNM組),M組進(jìn)行7天的Morris行為學(xué)實(shí)驗(yàn);N組(SDN組、NDN組、NNa N組、NDs N組、NDSN組、NDNN組),N組進(jìn)行7天的自然喂養(yǎng);總共12組(n=6)。分組后對SD大鼠進(jìn)行衰老造模(D-半乳糖,100mg/kg/d);在大鼠造模不同時期進(jìn)行有氧運(yùn)動,使用Morris水迷宮行為學(xué)測試檢測空間學(xué)習(xí)記憶能力;結(jié)束后檢測各組大鼠大腦中自由基相關(guān)指標(biāo)(超氧化物歧化酶SOD、谷胱甘肽過氧化酶GSH-PX、丙二醛MDA)的含量;使用real-time PCR、Western-blot等實(shí)驗(yàn)技術(shù)檢測各組大鼠小腦中BDNF的表達(dá)情況。結(jié)果:(1)SD大鼠NNa組大鼠狀態(tài)很好、行動快捷、生活飲食正常、毛發(fā)白亮;ND組的大鼠出現(xiàn)了明顯的自然衰老特征,毛發(fā)枯黃、行動遲緩、食欲欠佳、嗜睡。(2)自由基檢測結(jié)果:SDN組MDA的含量和SOD、GSH-Px的活性較NDN組差異無顯著性(P0.05);NDs N組MDA的含量顯著低于NDN組(P0.05),SOD(P0.05)、GSH-Px(P0.05)的活性顯著高于NDN組;NDSN組MDA含量顯著低于NDNN組(P0.05),SOD、GSH-Px活性顯著性高于NDNN組(P0.05)。(3)水迷宮定位航行實(shí)驗(yàn)結(jié)果:各組大鼠的平均逃避潛伏期隨著訓(xùn)練次數(shù)的增加逐漸減少且趨于穩(wěn)定,不同組別之間的平均逃避潛伏期具有一定的差異;各組在第2天的平均逃避潛伏期的時間比第1天少(P0.05);各組(除NDSM組)在第3天的平均逃避潛伏期的時間較第2天少(P0.05);NNa M組、NDs M組的平均逃避潛伏期的時間從第3天開始趨于穩(wěn)定(P0.05);但SDM組、NDM組、NDSM組、NDNM組的平均逃避潛伏期的時間在第4天才慢慢趨于穩(wěn)定(P0.05),經(jīng)過第1天的定位航行實(shí)驗(yàn),NNa M組的平均潛逃避伏期的時間較NDM組少(P0.05),NDSM組和NDNM組平均潛逃避伏期的時間慢慢增高(P0.05),在第6天的定位航行實(shí)驗(yàn)中,SDM組和NDM組平均潛逃避伏期的時間差異無顯著性(P0.05),與NDNM組相比,NDSM組的大鼠平均潛逃避伏期的時間顯著性降低(P0.05)�？臻g(第2象限入水)探索實(shí)驗(yàn)表明:SDM組穿越原站臺次數(shù)與NDM組差異無顯著性(P0.05);NDs M組穿越原站臺次數(shù)顯著高于NDM組(P0.05);NNa M組穿越原站臺次數(shù)顯著高于NDM組(P0.05);NDSM組穿越原站臺次數(shù)顯著高于NDNM組(P0.05)。(4)小腦BDNF real-time PCR和Western-blot檢測結(jié)果:NNa N組小腦BDNF m RNA(P0.05)和蛋白的表達(dá)量(P0.05)顯著高于NDN組;SDN組小腦BDNF m RNA較NDN組差異無顯著性(P0.05),SDN組小腦BDNF蛋白質(zhì)的表達(dá)量較NDN組差異無顯著性(P0.05);NDs N組小腦BDNF m RNA(P0.05)和蛋白的表達(dá)量(P0.05)顯著性高于NDN組,NDSN組小腦BDNF m RNA表達(dá)顯著性高于NDNN組(P0.05),蛋白質(zhì)量的表達(dá)顯著性高于NDNN組(P0.05),M組與其相對應(yīng)N組變化趨勢相同,組間無顯著差異(P0.05)。結(jié)論:(1)造模后SD大鼠的形態(tài)學(xué)特征和各自由基相關(guān)指標(biāo)證明衰老模型造模成功。(2)造模前對SD大鼠進(jìn)行有氧運(yùn)動干預(yù),大鼠小腦BDNF的表達(dá)沒有上調(diào),大鼠空間學(xué)習(xí)記憶能力沒有明顯的變化。(3)在造模過程中和造模后對SD大鼠進(jìn)行有氧運(yùn)動干預(yù),能上調(diào)大鼠小腦BDNF的表達(dá),提高大鼠空間學(xué)習(xí)記憶能力。(4)實(shí)驗(yàn)證明有氧運(yùn)動上調(diào)大鼠小腦BDNF的表達(dá),可能是延緩大鼠空間學(xué)習(xí)記憶能力衰退的機(jī)制之一。
[Abstract]:Objective: SD male rats were intraperitoneally injected with D- galactose to make aging model. In the process of model building, aerobic exercise (swimming, 60min/d) was used to intervene in the rat model before model building, and the index of brain senescence, spatial learning and memory, and the expression of cerebellar neurotrophic factor BDNF were detected in rats, and the brain senescence and learning and memory of aerobic exercise on rat brain were discussed. Methods: the effects of ability and cerebellar BDNF. Methods: the SD rats (72, 3 month old, male) were randomly divided into 6 large groups by SD (pre model aerobic exercise preconditioning group), ND (model group), NNa (injection physiological saline control group), NDs (aerobic exercise group during molding process), NDS (after model oxygenation group) and NDN (after model natural feeding group). Each group was randomly divided into 2 groups: group M (group SDM, group NDM, NNa M group, NDs M group, NDSM group, NDNM group), M group for Morris behavioral experiment for 7 days; N groups were fed with 7 days of natural feeding. Aerobic exercise was carried out at different stages and the Morris water maze behavior test was used to test the spatial learning and memory ability. After the end, the content of free radical related indexes (superoxide dismutase SOD, glutathione peroxidase GSH-PX, malondialdehyde MDA) in the brain of each group was detected, and real-time PCR, Western-blot and other experimental techniques were used to detect each group. The expression of BDNF in the cerebellum of rats. Results: (1) the rats in group NNa of SD were in good state, quick action, normal diet and white hair; the rats in group ND had obvious natural aging characteristics, hair yellow, slow action, poor appetite, lethargy. (2) the content of MDA in group SDN and SOD, GSH-Px activity was more than NDN group. The content of MDA in group NDs N was significantly lower than that of NDN group (P0.05), SOD (P0.05), GSH-Px (P0.05) activity was significantly higher than that of NDN group, and the activity of NDSN group was significantly lower than that of the NDN group. (3) the results of water maze navigation experiment: the average escape latency of rats in each group was trained with the training. The average evasion latency between different groups had a certain difference, the average escape latency of each group was less than that of first days (P0.05), and the average escape latency of each group (NDSM group) at third days was less than that of second days (P0.05), and the average escape latency in group NNa M and NDs M group. The period of time began to stabilize from third days (P0.05), but the average escape latency of group SDM, NDM, NDSM and NDNM gradually stabilized at fourth days (P0.05). After first days of navigation experiment, the average escape time of NNa M group was less than that of the NDM group (P0.05), while the average escape time of NDSM and NDNM groups was slow. Man Zenggao (P0.05), in the sixth day positioning navigation experiment, the time difference between the SDM group and the NDM group had no significant difference (P0.05). Compared with the NDNM group, the average escape time of the rats in the NDSM group was significantly lower (P0.05). The space (second quadrant water entry) exploration experiment showed that the number of SDM groups crossing the original platform was not different from the NDM group. The number of NDs M traversing the original platform was significantly higher than that of the NDM group (P0.05), and the number of NNa M groups crossing the original platform was significantly higher than that of the NDM group (P0.05), and the number of NDSM groups across the original platform was significantly higher than that of the NDNM group (P0.05). (4) the expression of cerebellar cerebellum and protein was significantly higher than that of the NDNM group (4). There was no significant difference between the cerebellar BDNF m RNA in the SDN group and the NDN group (P0.05), and there was no significant difference in the expression of BDNF protein in the cerebellum of SDN group compared with that of the NDN group (P0.05). The significant sex of the cerebellar cerebellar and protein expression was significantly higher than that of the NDN group (P0.05). The significance of the expression was higher than that of the NDNN group (P0.05), and there was no significant difference between the M group and the corresponding N group (P0.05). Conclusion: (1) the morphological characteristics of the SD rats and the related indexes of the free radicals proved that the model of the aging model was successful. (2) the aerobic exercise intervention was carried out on SD rats before the modeling, and the expression of BDNF in the cerebellum of the rat was not up. There was no obvious change in the ability of spatial learning and memory in rats. (3) aerobic exercise intervention on SD rats in the process of modeling and after modeling could increase the expression of BDNF in the cerebellum of rats and improve the spatial learning and memory ability of rats. (4) the experiment showed that aerobic exercise increased the expression of BDNF in the rat cerebellum, which may delay the spatial learning and memory ability of rats. One of the mechanisms of the recession.
【學(xué)位授予單位】：成都體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R87

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