本文選題：有氧運(yùn)動(dòng) + Nrf��； 參考：《中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志》2017年10期

【摘要】：目的:探討不同時(shí)間一次有氧運(yùn)動(dòng)對(duì)小鼠骨骼肌NF-E2相關(guān)因子(NF-E2-related factor 2,Nrf2)與Kelch樣環(huán)氧氯丙烷相關(guān)蛋白-1(Kelch-like ECH-associated protein-1,Keapl)結(jié)合作用的影響。方法:C57BL/6J小鼠30只,按體重隨機(jī)分為安靜對(duì)照組(0 h組)和一次有氧運(yùn)動(dòng)3小時(shí)組(3 h組)、一次有氧運(yùn)動(dòng)6小時(shí)組(6 h組),每組10只。0 h組安靜飼養(yǎng),3 h組和6 h組小鼠分別進(jìn)行3小時(shí)和6小時(shí)的一次有氧跑臺(tái)訓(xùn)練,跑速為15 m/min,坡度為0。運(yùn)動(dòng)后即刻取后腿骨骼肌。高質(zhì)熒光測(cè)定法檢測(cè)小鼠骨骼肌活性氧(ROS)水平;免疫共沉淀法測(cè)Nrf2/Keap1結(jié)合量;Western Blot法測(cè)定骨骼肌總Nrf2、Keap1蛋白表達(dá)量和細(xì)胞核Nrf2蛋白表達(dá)量。結(jié)果:與0 h組相比,6 h組ROS水平顯著高于0 h組(P0.05);3h、6 h組小鼠骨骼肌中Nrf2/Keap1結(jié)合量顯著降低(P0.05);3 h、6 h組小鼠骨骼肌總Nrf2蛋白表達(dá)顯著升高(P0.05),6 h組小鼠骨骼肌細(xì)胞核Nrf2蛋白表達(dá)顯著增加且與3h組相比也顯著增加(P0.05);小鼠骨骼肌總Keap1蛋白表達(dá)基本無(wú)變化。結(jié)論:一次有氧運(yùn)動(dòng)對(duì)小鼠骨骼肌Nrf2與Keap1解綁和Nrf2轉(zhuǎn)位入核的影響與運(yùn)動(dòng)時(shí)間的長(zhǎng)短有關(guān)。
[Abstract]:Aim: to investigate the effect of aerobic exercise at different times on the binding of NF-E2 related factor NF-E2-related factor 2nrf2) to Kelch like epichlorohydrin associated protein-1 Kelch-like ECH-associated protein-1 Keapl. in mice skeletal muscle. Methods 30 C57BL / 6J mice, According to body weight, they were randomly divided into three groups: the control group (0 h), the 3 h aerobic exercise group (3 h group) and the 6 hour aerobic exercise group (6 h group). 10 rats in each group were fed quietly for 3 h and 6 h group respectively An hour and six hours of aerobic treadmill training, Running at a speed of 15 m / min, with a slope of 0. Take back leg skeletal muscle immediately after exercise. High quality fluorescence assay was used to detect the level of reactive oxygen species (Ros) in skeletal muscle of mice, and the expression of total Nrf2Keap1 protein and nuclear Nrf2 protein were measured by immunoprecipitation method and Western Blot method. Results: compared with the 0 h group, the ROS level of the 6 h group was significantly higher than that of the 0 h group P0.05 + 3 h group. The Nrf2/Keap1 binding capacity in the skeletal muscle of the mice was significantly lower than that of the 0 h group. The expression of total Nrf2 protein in the skeletal muscle of the mice in the P0. 05 h group was significantly higher than that in the 0 h group. The expression of total Nrf2 protein in the skeletal muscle of the 6 h group was significantly higher than that of the 0 h group. Compared with the 3h group, the protein expression increased significantly, and the expression of total Keap1 protein in the skeletal muscle of mice showed no change. Conclusion: the effect of one aerobic exercise on Nrf2 and Keap1 untying and Nrf2 transposition into nucleus is related to the duration of exercise.
【作者單位】： 北京體育大學(xué);
【基金】：國(guó)家自然科學(xué)基金(31471134) 北京市支持中央在京高校共建項(xiàng)目、運(yùn)動(dòng)與體質(zhì)健康教育部重點(diǎn)實(shí)驗(yàn)室、運(yùn)動(dòng)人體科學(xué)國(guó)家級(jí)實(shí)驗(yàn)教學(xué)示范中心(北京體育大學(xué))資助
【分類(lèi)號(hào)】：R87

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