本文選題：細(xì)胞培養(yǎng)板 + 流路控制擋板��； 參考：《航天醫(yī)學(xué)與醫(yī)學(xué)工程》2017年02期

【摘要】：目的為適用于空間特殊實(shí)驗(yàn)環(huán)境,并在細(xì)胞培養(yǎng)實(shí)驗(yàn)中更高效地使用試劑、提高細(xì)胞培養(yǎng)用品的污染防護(hù)能力,提出了一種內(nèi)部流動路徑可控、具備防滲漏、防污染能力的全透明和全封閉細(xì)胞培養(yǎng)板。方法基于有限元仿真對培養(yǎng)板核心結(jié)構(gòu)—流路控制擋板進(jìn)行了優(yōu)化,以優(yōu)化后的培養(yǎng)板為核心部件搭建細(xì)胞培養(yǎng)回路,并開展了中長周期細(xì)胞培養(yǎng)試驗(yàn)。結(jié)果最優(yōu)化的三壩-縱向排列細(xì)胞培養(yǎng)板,可有效控制其內(nèi)部流體的流動路徑,進(jìn)出口流速1 m L/min時流體剪切力僅4.8×10~(-5)Pa,流動死區(qū)幾乎為0,以該培養(yǎng)板為核心構(gòu)建的細(xì)胞培養(yǎng)回路中培養(yǎng)的細(xì)胞可正常生長15 d。結(jié)論該細(xì)胞培養(yǎng)板,可使腔內(nèi)新舊細(xì)胞培養(yǎng)試劑更換及殘留氣體排出更徹底,防滲漏防污染能力大大提升,試劑使用率提高,能滿足空間實(shí)驗(yàn)常用典型細(xì)胞系的中長期培養(yǎng)需求,適合于我國目前及將來的空間醫(yī)學(xué)生物學(xué)實(shí)驗(yàn)應(yīng)用場合。
[Abstract]:Objective in order to be suitable for special space experimental environment, to use reagents more efficiently in cell culture experiment, and to improve the pollution protection ability of cell culture products, a kind of internal flow path is controlled and anti-leakage is proposed. Anti-fouling ability of fully transparent and fully enclosed cell culture plates. Methods based on the finite element simulation, the core structure of the culture plate, the flow control baffle, was optimized. The cell culture loop was set up with the optimized culture plate as the core component, and the medium and long period cell culture experiment was carried out. Results the optimized three-dam-longitudinal cell culture plate can effectively control the flow path of the internal fluid. At the flow rate of 1 m L/min, the flow shear stress was only 4.8 脳 10 ~ (-5) Pa, and the flow dead zone was almost zero. The cells cultured in the cell culture loop constructed with the culture plate as the core could grow normally for 15 days. Conclusion the new and old cell culture reagents can be replaced and the residual gas can be discharged more thoroughly, the ability of preventing leakage and pollution can be greatly improved, and the utilization rate of reagents can be increased by using the new and old cell culture plates. It can meet the medium and long term culture needs of typical cell lines in space experiments, and is suitable for the present and future applications of space medical biology experiments in China.
【作者單位】： 中國航天員科研訓(xùn)練中心航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國家重點(diǎn)實(shí)驗(yàn)室資助項(xiàng)目(SMFA13A03,SMFA15A02)
【分類號】：R85

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