發(fā)布時(shí)間：2018-04-10 02:23

  本文選題：閉合性骨折　切入點(diǎn)：機(jī)械振動(dòng)　出處：《陜西師范大學(xué)》2012年碩士論文

【摘要】：目的：探討機(jī)械振動(dòng)協(xié)同骨髓干細(xì)胞(Bone marrow stem cells, BMSCs)動(dòng)員對(duì)大鼠閉合性骨折愈合血管生成的影響及其可能機(jī)制。 方法：清潔級(jí)3月齡Sprague-Dawley雌性大鼠160只,體重200±20g。隨機(jī)分為4組,即骨折模型組(C組),骨折+動(dòng)員劑組(M組),骨折+振動(dòng)組(V組),骨折+動(dòng)員劑+振動(dòng)組(M+V組),每組40只。V組、M+V組骨折72h后接觸30Hz累計(jì)15min/d,5d/W的間歇振動(dòng)。M組和M+V組骨折3h后背部皮下注射重組人粒細(xì)胞集落刺激因子(rhG-CSF,5μg/kg/d),共5d,各組分別在第1w/2w/4w/5w取材。免疫組化檢測(cè)組織中CD31、CD34、VEGF、ANG-1和FⅧ-Rag蛋白表達(dá),免疫印跡法分析愈合期骨組織中ANG-1和VEGF蛋白表達(dá)。 實(shí)驗(yàn)結(jié)果： 本實(shí)驗(yàn)采用大鼠骨折后rhG-CSF動(dòng)員的方法來(lái)動(dòng)員干細(xì)胞,促進(jìn)BMSCs向骨外周血遷移,增加外周血的BMSCs,加速血管再生,改善局部血液供應(yīng)促進(jìn)骨折愈合。采用機(jī)械振動(dòng),加速局部血液循環(huán),促進(jìn)血管再生與重建,通過(guò)機(jī)械振動(dòng)改善骨折周?chē)W(xué)環(huán)境,為骨折愈合提供合適的微環(huán)境。其結(jié)果如下： 1用大鼠骨折造模支架造成大鼠股骨中段閉合性骨折,通過(guò)X光片判斷,成功建立大鼠骨折模型。 2機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員均可促進(jìn)大鼠骨折愈合與功能恢復(fù)。但機(jī)械振動(dòng)和rhG-CSF動(dòng)員雙重作用效果更為顯著,優(yōu)于骨折后單純r(jià)hG-CSF動(dòng)員作用或單純機(jī)械振動(dòng)作用。 3機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員對(duì)大鼠骨折恢復(fù)過(guò)程中的血管再生及骨組織形成有一定促進(jìn)作用。機(jī)械振動(dòng)或rhG-CSF動(dòng)員可使毛細(xì)血管數(shù)量增多,血管重建加速,較早地形成血管網(wǎng)；尤其以機(jī)械振動(dòng)和機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員效果更明顯。提示：骨折后進(jìn)行機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員可促進(jìn)骨折愈合早期的血管形成,改善骨折部位的微環(huán)境,促進(jìn)骨折愈合。 4機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員對(duì)大鼠骨折部位BMSCs動(dòng)員顯著。CD34+在各組實(shí)驗(yàn)大鼠骨折部位表達(dá)先升高后降低,第2周時(shí)是4次取材峰值,機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員組機(jī)械振動(dòng)組rhG-CSF動(dòng)員組模型組。提示：機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員雙重作用加強(qiáng)了BMSCs的動(dòng)員,可趨化募集更多的BMSCs,加速骨折部位的損傷修復(fù)。 5機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員可促進(jìn)VEGF的表達(dá),機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員的雙重作用效果更加顯著。VEGF在各組實(shí)驗(yàn)大鼠骨折部位表達(dá)先升高后降低,第2周時(shí)是4次取材峰值,機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員組機(jī)械振動(dòng)組rhG-CSF動(dòng)員組模型組。 6機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員均可促進(jìn)Ang-1的表達(dá),機(jī)械振動(dòng)組、rhG-CSF動(dòng)員組和模型組大鼠骨折部位表達(dá)規(guī)律為先升高后降低,第4周時(shí)是4次取材峰值,而機(jī)械振動(dòng)和rhG-CSF動(dòng)員雙重作用組第2周時(shí)是4次取材的峰值,之后逐次下降,表現(xiàn)為機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員組機(jī)械振動(dòng)組rhG-CSF動(dòng)員組模型組；機(jī)械振動(dòng)和rhG-CSF動(dòng)員雙重作用刺激Ang-1表達(dá)最顯著。 7機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員均可促進(jìn)FⅧ-Rag、CD31的表達(dá),可反映大鼠骨折愈合過(guò)程中的血管新生。骨折愈合的血管再生高峰期在第2-4周,且機(jī)械振動(dòng)協(xié)同rhG-CSF動(dòng)員組機(jī)械振動(dòng)組rhG-CSF動(dòng)員組模型組。提示,BMSCs動(dòng)員和機(jī)械振動(dòng)以及二者雙重作用均可促進(jìn)缺血組織血管生成,改善缺血區(qū)的血液灌注,加速骨折愈合。 8血管生成素Ang-1的WB結(jié)果顯示,正常情況下Ang-1表達(dá)量較少,骨折1周達(dá)高峰；動(dòng)員劑組表達(dá)量呈逐漸升高,表達(dá)量多于安靜組；振動(dòng)組早期明顯升高后呈自然下降規(guī)律,但表達(dá)量較多；動(dòng)員振動(dòng)組則從第2周到4周明顯升高,5周時(shí)表達(dá)再次顯著下降,但各期A(yíng)ng-1表達(dá)量明顯高于其他組。表明在骨折愈合期,機(jī)械振動(dòng)和動(dòng)員劑均可促進(jìn)Ang-1表達(dá),機(jī)械振動(dòng)作用優(yōu)于動(dòng)員劑,早期二者合用促進(jìn)血管生成效果顯著。機(jī)械振動(dòng)與動(dòng)員劑合用效果更加顯著,作用優(yōu)于單一因素。 9血管內(nèi)皮生長(zhǎng)因子VEGF的WB結(jié)果顯示,正常情況下VEGF表達(dá)較少；動(dòng)員劑組表達(dá)量逐漸升高,但表達(dá)量多于安靜組；振動(dòng)組早期明顯升高后呈自然降低,但表達(dá)量較多；動(dòng)員振動(dòng)組表達(dá)量先降低后升高,但各期VEGF表達(dá)量明顯高于其他組。表明在骨折愈合期,機(jī)械振動(dòng)和動(dòng)員劑均可促進(jìn)VEGF表達(dá),機(jī)械振動(dòng)作用優(yōu)于動(dòng)員劑,在早期兩者合用促進(jìn)血管生成效果顯著。機(jī)械振動(dòng)與動(dòng)員劑合用效果更加顯著,作用優(yōu)于單一因素。 結(jié)論： 機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員可顯著增加大鼠骨折部位BMSCs標(biāo)志物、血管再生因子、血管新生因子的表達(dá),但機(jī)械振動(dòng)和rhG-CSF動(dòng)員雙重作用效果更為顯著。提示,機(jī)械振動(dòng)和(或)rhG-CSF動(dòng)員可顯著促進(jìn)BMSCs動(dòng)員,促進(jìn)血管再生,促進(jìn)骨折修復(fù)。機(jī)械振動(dòng)協(xié)同BMSCs動(dòng)員是促進(jìn)骨折愈合的較好方法。
[Abstract]:Objective : To investigate the effects of mechanical vibration and bone marrow stem cells ( BMSC ) mobilization on the angiogenesis of closed fracture healing in rats and its possible mechanism .

Methods : 160 male Sprague - Dawley female rats weighing 200 鹵 20g were randomly divided into four groups : fracture model group ( group C ) , fracture + mobilization agent group ( M group ) , fracture + vibration group ( group V ) , fracture + mobilization agent + vibration group ( M + V group ) .

Experimental results :

We use the method of rhG - CSF mobilization after fracture of rats to mobilize the stem cells , promote the migration of bone marrow into the peripheral blood of the bone , increase the peripheral blood cells , accelerate the blood vessel regeneration , improve the local blood supply and promote the fracture healing . The mechanical vibration is used to accelerate the local blood circulation , promote the vascular regeneration and reconstruction , improve the surrounding mechanical environment of the fracture by mechanical vibration , and provide a suitable microenvironment for the fracture healing .

1 Rat fracture model was successfully established by X - ray film .

2 Mechanical vibration and ( or ) rhG - CSF mobilization can promote fracture healing and functional recovery in rats , but the effects of mechanical vibration and rhG - CSF mobilization are more remarkable than that of simple rhG - CSF mobilization or simple mechanical vibration after fracture .

3 . Mechanical vibration and / or rhG - CSF mobilization can promote the regeneration of blood vessels and the formation of bone tissue during the recovery of fracture of rats . Mechanical vibration or rhG - CSF mobilization can increase the number of capillaries and accelerate the reconstruction of blood vessels , thus forming the vascular network early .
It is suggested that mechanical vibration and rhG - CSF mobilization can accelerate the formation of blood vessels in the early stage of fracture healing , improve the microenvironment of fracture site and promote fracture healing .

4 mechanical vibrations and ( or ) rhG - CSF mobilization significantly increased the mobilization of bone marrow cells in the fracture site of rats . The expression of CD34 + in the fracture sites of the rats was increased first and then decreased at the second week . The mechanical vibration was combined with rhG - CSF to mobilize the rhG - CSF mobilization group model group .

5 Mechanical vibration and ( or ) rhG - CSF mobilization could promote the expression of VEGF . The effects of mechanical vibration and rhG - CSF mobilization were more significant .

Both mechanical and rhG - CSF mobilization could promote the expression of Ang - 1 , the mechanical vibration group , the rhG - CSF mobilization group and the model group rats .
Mechanical vibration and rhG - CSF mobilized double action to stimulate the expression of Ang - 1 .

7 mechanical vibration and ( or ) rhG - CSF mobilization can promote the expression of F鈪，

本文編號(hào)：1729247