本文選題：有氧運動　切入點：BKca通道　出處：《北京體育大學》2012年碩士論文

【摘要】：研究目的：觀察有氧運動對大鼠腦動脈平滑肌細胞BKCa通道生物物理特性的影響。 研究方法：8周齡雄性Wistar大鼠30只,隨機分為三組,安靜對照組(SED),高頻運動組(EX-5d／w)和低頻運動組(EX-3d／w)各10只。運動組進行12周跑臺運動(0°slope,20m、min,50-55％VO2max,60min/day),其中EX-3d／w組每周運動3次,EX-5d／w組每周運動5次。12周后,將大鼠麻醉,斷頭處死,迅速打開顱腔取腦。小心取出基底動脈及Willis環(huán),剝除周圍結(jié)締組織,經(jīng)酶法消化后得到單個腦動脈平滑肌細胞。膜片鉗實驗采用單通道內(nèi)面向外模式記錄,觀察各組大鼠腦動脈BKCa通道的生物物理特性。 研究結(jié)果：(1)BKCa通道鑒定：本實驗單通道電導(dǎo)為210.5±10,3pS(R2=0.996,n=8)；記錄電流反轉(zhuǎn)電位接近OmV,無整流現(xiàn)象,均符合BKCa單通道特征。(2)有氧運動使BKCa通道活性提高：膜電位+40mV,[Ca2+]i為1μM時,SED、EX一3d/w和EX-5d／w各組BKCa通道平均開放概率分別為0.28±0.015,0.47±0.02,0.78±0.067。(3)有氧運動使BKCa通道電壓依賴性增強：[Ca2+]i為1μM時,SED. EX-3d/w. EX-5d/w三組(Normal ized Npo)-V曲線依次左移,三組V1／2分別為44.52mV,37.08mV,26.55mV.(4)有氧運動使BKCa通道鈣敏感性增強：膜電位+40mV下,SED、EX-3d/w. EX-5d/w三組(Normalized NPo)-Log[Ca2+](?)曲線依次左移,Kd值分別為2.801±0.086μM,1.39±0.079μM,0.35±0.087μM。(5)運動組大鼠BKCa通道平均開放時間(To)增大;平均關(guān)閉時間(Tc)減小,提示有氧運動使通道更易開放且更難關(guān)閉。 結(jié)論：長期規(guī)律性有氧運動能改變大鼠腦動脈平滑肌細胞BKCa通道的門控特性,使其活性增強,這可能是運動改善腦血管舒縮功能,增強腦動脈供血.的重要機制之一；有氧運動改善BKCa通道活性的效果可能呈運動量依賴性。
[Abstract]:Aim: to observe the effects of aerobic exercise on the biophysical properties of BKCa channels in rat cerebral artery smooth muscle cells.Methods Thirty eight week-old male Wistar rats were randomly divided into three groups: the quiet control group, the high frequency exercise group (EX-5d / w) and the low-frequency exercise group (EX-3d / w).The rats in the exercise group were treated with 0 擄slopetrope (20 擄slopetrol) for 12 weeks. The rats in the EX-3d/w group were treated with EX-5 d / w 3 times a week for 5 times a week. The rats were anesthetized and killed by severed, and the brain was quickly opened to the cranial cavity for brain extraction. The rats in the EX-3d/w group were treated with EX-5d / w exercise three times a week. The rats were anesthetized, killed and quickly opened to the skull for brain extraction.The basilar artery and Willis ring were carefully removed and the connective tissue around them was stripped. The smooth muscle cells of single cerebral artery were obtained by enzymatic digestion.Single channel inward and outward mode was used to observe the biophysical characteristics of BKCa channel in the cerebral artery of rats in each group.The results showed that the single channel conductance of this experiment was 210.5 鹵10 ~ 3pSN ~ (2) 0.996N ~ (-1) N ~ (8), the current reversal potential was close to OmV, and there was no rectifying phenomenon.EX-3 d / w.The normal ized Npo)-V curve of the three groups of EX-5d/w shifted to the left in turn. The V1 / 2 of the three groups was 44.52 MV, 37.08 MV, 26.55 MV. 4) aerobic exercise enhanced the calcium sensitivity of the BKCa channel: SEDX EX-3 d / w at the membrane potential 40mV.EX-5d/w NPo)-Log [Ca2]The mean opening time of BKCa channel increased and the mean closing time decreased in the exercise group, indicating that aerobic exercise could make the channel more open and difficult to close. The curve shifted to the left and the KD value was 2.801 鹵0.086 渭 MU 1.39 鹵0.079 渭 MU 0.35 鹵0.087 渭 M.M.) in the exercise group, the mean opening time of the BKCa channel increased and the mean closing time decreased, indicating that aerobic exercise made the channel easier to open and harder to close.Conclusion: Long-term regular aerobic exercise can change the gated characteristics of BKCa channel in rat cerebral artery smooth muscle cells and increase its activity, which may be due to the improvement of cerebral vasomotor function and the enhancement of cerebral artery blood supply by exercise.The effect of aerobic exercise on improving BKCa channel activity may be in a dose-dependent manner.
【學位授予單位】：北京體育大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：G804.2

【相似文獻】

相關(guān)期刊論文 前10條

1 ;時尚潮流,有氧運動![J];人生十六七;2007年10期

2 孫豪甫;;老人健康離不開有氧運動[J];食品與健康;2011年09期

3 錢程;;運動有助于睡眠[J];心血管病防治知識;2010年12期

4 周音蓮;胡海燕;張荷;周磊;;有氧運動在療養(yǎng)期間的應(yīng)用與作用[J];中國療養(yǎng)醫(yī)學;2011年07期

5 葉新平;;原發(fā)性高血壓的運動處方研究[J];文體用品與科技;2011年08期

6 胡綺雯;;跳起輕快的華爾茲,走向更強健的心臟[J];心血管病防治知識;2007年04期

7 ;研究發(fā)現(xiàn)有氧運動可抑制非酒精性脂肪肝[J];藥物與人;2011年07期

8 岳靜靜;吳衛(wèi)東;;不同體脂分布對青年肥胖女性有氧運動后血清IL-6水平的影響[J];少林與太極(中州體育);2011年01期

9 任彩玲;余志琪;張鈞;;有氧運動對自發(fā)性高血壓大鼠心肌血紅素氧合酶活性及血紅素氧合酶-1mRNA表達的影響[J];中國康復(fù)醫(yī)學雜志;2011年07期

10 貴永玲;;有氧運動對中老年機體機能影響的生理學分析[J];少林與太極(中州體育);2009年12期

相關(guān)會議論文 前10條

1 蓉英;柳維林;;中等強度有氧運動對肥胖大鼠血清HDL及LDL水平的改變[A];湖南省首屆生理—藥理科學青年論壇論文摘要匯編[C];2009年

2 趙瑾;劉華平;陳京立;劉穎;;居家有氧運動對乳腺癌門診化療患者癌因性疲乏的干預(yù)效果[A];中華護理學會2008“海峽兩岸護理青年科學家”學術(shù)研討會論文匯編[C];2008年

3 劉洪珍;孔喜良;;有氧運動對人體谷胱甘肽抗氧化系統(tǒng)影響的研究[A];2002年第9屆全國運動醫(yī)學學術(shù)會議論文摘要匯編[C];2002年

4 張一民;邢文華;章嵐;李爽;;長期有氧運動對增齡大鼠肝細胞凋亡及相關(guān)基因表達的影響[A];第七屆全國體育科學大會論文摘要匯編（二）[C];2004年

5 黃治官;郝選明;;有氧運動與衰竭心肌細胞能量代謝[A];2008年神經(jīng)內(nèi)分泌暨神經(jīng)免疫內(nèi)分泌學術(shù)研討會論文摘要匯編[C];2008年

6 陸愛云;袁凌燕;;有氧運動改善血管內(nèi)皮受損干預(yù)動脈粥樣硬化形成的實驗研究[A];第八屆全國體育科學大會論文摘要匯編（一）[C];2007年

7 趙斐;張娜;張勇;;有氧運動對飲食性高脂血癥大鼠肝臟HMG-CoA還原酶基因表達的影響[A];2002年第9屆全國運動醫(yī)學學術(shù)會議論文摘要匯編[C];2002年

8 姚保龍;金冬云;霍文t，

本文編號：1723787