本文關(guān)鍵詞： 活性氧 一氧化氮 過(guò)氧亞硝酸陰離子 力竭運(yùn)動(dòng)　出處：《湖南大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：運(yùn)動(dòng)可以誘導(dǎo)自由基的生成，這一現(xiàn)象已被發(fā)現(xiàn)了三十余年，但其中的機(jī)制尚不清楚。大量研究已證實(shí)運(yùn)動(dòng)可導(dǎo)致體內(nèi)超氧陰離子（O_2-）的生成，也有研究表明運(yùn)動(dòng)也導(dǎo)致骨骼肌一氧化氮合酶活性的增強(qiáng)，進(jìn)而一氧化氮（NO）生成增加，而NO與O_2-能迅速反應(yīng)形成過(guò)氧亞硝酸陰離子（ONOO~-）。ONOO~-是極強(qiáng)的氧化劑，推測(cè)其對(duì)運(yùn)動(dòng)性疲勞的產(chǎn)生起著至關(guān)重要的作用。 本文以大鼠力竭運(yùn)動(dòng)為模型，誘發(fā)大鼠運(yùn)動(dòng)性疲勞為切入點(diǎn)，觀察血清中各項(xiàng)自由基相關(guān)指標(biāo)和代謝程度。以求更加深入、系統(tǒng)地探明是否力竭運(yùn)動(dòng)誘導(dǎo)血清ONOO~-的過(guò)量生成，為運(yùn)動(dòng)運(yùn)動(dòng)性疲勞的自由基學(xué)說(shuō)提供新的理論依據(jù)。 研究方法： 32只6周齡雄性SD大鼠，分成對(duì)照組（C組）和力竭運(yùn)動(dòng)組（E組）。E組以運(yùn)動(dòng)跑臺(tái)構(gòu)筑力竭運(yùn)動(dòng)模型。力竭后即刻取樣檢測(cè)。 研究結(jié)果： 1.經(jīng)力竭運(yùn)動(dòng)后，E組血清CK活性增加14%（P0.05），，而血清LDH沒(méi)有顯著性差異。 2.E組血清MDA明顯增加42%（P0.05），而血清SOD活力降低了300%（P0.05）。 3.經(jīng)力竭運(yùn)動(dòng)后，血清NO濃度升高了8%，ONOO~-更是升高18%（P0．01）。 研究結(jié)論： 1.力竭運(yùn)動(dòng)導(dǎo)致血清CK活性上升，表明骨骼肌細(xì)胞損傷，大量骨骼肌細(xì)胞內(nèi)的CK滲漏進(jìn)入血液，產(chǎn)生運(yùn)動(dòng)性疲勞。 2.力竭運(yùn)動(dòng)使機(jī)體生成過(guò)量的自由基，從而使細(xì)胞脂質(zhì)過(guò)氧化反應(yīng)加強(qiáng)，生成大量MDA；同時(shí)，血清SOD活性降低，反映出SOD因清除自由基而消耗。這兩個(gè)指標(biāo)的變化也間接反映了力竭運(yùn)動(dòng)導(dǎo)致自由基的過(guò)量生成。 3.ONOO~-的升高其原因可能是大量的O_2-與NO反應(yīng)生成，ONOO~-的生成可能是運(yùn)動(dòng)性疲勞的機(jī)制之一。
[Abstract]:It has been discovered for more than 30 years that exercise can induce free radical formation, but the mechanism is still unclear. A large number of studies have proved that exercise can lead to the generation of superoxide anion O _ 2-in vivo. Studies have also shown that exercise also leads to an increase in the activity of nitric oxide synthase in skeletal muscles, which in turn increases the production of nitric oxide nitrite. No and O _ 2- can react rapidly to form the peroxynitrite anion, ONOOO-, which is an extremely strong oxidant. It is inferred that it plays an important role in the generation of exercise fatigue. In this paper, using exhaustive exercise in rats as a model and inducing exercise-induced fatigue in rats as a cut-in point, we observed the related indexes of free radicals and the degree of metabolism in the serum, in order to find out whether exhaustive exercise induced the excessive production of ONOO- in serum. It provides a new theoretical basis for the free radical theory of exercise fatigue. Research methods:. Thirty-two 6-week-old male Sprague-Dawley rats were divided into control group (C group) and exhaustive exercise group (group E). Results of the study:. 1. After exhaustive exercise, the serum CK activity in E group increased by 14% (P 0.05), but there was no significant difference in serum LDH. 2. In E group, the serum MDA increased significantly (P 0.05), while the serum SOD activity decreased (P 0.05). 3. After exhaustive exercise, the concentration of no in serum increased by 8% and increased by 18% (P 0.01). The study concluded that:. 1.Exercise-induced serum CK activity increased, indicating that skeletal muscle cells were injured, a large number of CK leakage into the blood, resulting in exercise fatigue. 2. Exhausted exercise resulted in excessive production of free radicals, which enhanced cellular lipid peroxidation and produced a large number of MDAs, and decreased the activity of serum SOD. The results show that SOD is consumed by scavenging free radicals, and the changes of these two indexes also indirectly reflect the excessive production of free radicals caused by exhaustive exercise. 3. The increase of ONOO- may be due to the formation of a large number of O2s, which react with no to produce ONOOO-, which may be one of the mechanisms of exercise-induced fatigue.
【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R87

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 熊正英,曲洪剛;一氧化氮與運(yùn)動(dòng)疲勞關(guān)系的研究進(jìn)展[J];福建體育科技;2004年02期

2 李澤清;侯天德;;運(yùn)動(dòng)性內(nèi)源活性氧產(chǎn)生及對(duì)線粒體DNA氧化損傷[J];甘肅科技;2008年22期

3 張艷;梁標(biāo);;磷脂酶A_2的調(diào)節(jié)機(jī)制[J];吉林醫(yī)學(xué);2005年12期

4 郭光明;;8周跑臺(tái)運(yùn)動(dòng)對(duì)大鼠血清LDH、LA、CK影響的研究[J];遼寧體育科技;2011年02期

5 呂超,魏彥林,林金明;過(guò)氧亞硝酸鹽的化學(xué)特性與化學(xué)發(fā)光反應(yīng)[J];世界科技研究與發(fā)展;2004年04期

6 凌亦凌,黃善生,谷振勇;過(guò)氧亞硝基陰離子的細(xì)胞代謝及病理?yè)p傷作用[J];生理科學(xué)進(jìn)展;1999年01期

7 陳敏,李強(qiáng),陳彬;過(guò)氧亞硝基陰離子的研究進(jìn)展[J];生命的化學(xué);2003年06期

8 王瑾雯,陳瑗,周玫;一氧化氮、超氧陰離子和過(guò)氧亞硝酸的作用和相互關(guān)系的研究進(jìn)展[J];生命的化學(xué)(中國(guó)生物化學(xué)會(huì)通訊);1998年01期

9 曹啟花;鄭琦;劉志洪;蔡汝秀;;ONOO~-活性氧的熒光光譜分析進(jìn)展[J];武漢大學(xué)學(xué)報(bào)(理學(xué)版);2006年06期

10 付毅,楊建昌;一氧化氮及其合酶與運(yùn)動(dòng)的關(guān)系[J];西安體育學(xué)院學(xué)報(bào);1998年04期

相關(guān)碩士學(xué)位論文 前2條

1 榮琳;過(guò)氧亞硝酸陰離子在肝性腦病中作用的研究[D];山東大學(xué);2010年

2 王丹;運(yùn)動(dòng)導(dǎo)致活性氧增加的NADPH氧化酶途徑研究[D];湖南大學(xué);2011年

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