【摘要】：目的:探討環(huán)境內(nèi)分泌干擾物全氟辛磺酸(perfluorooctane suifonate,PFOS)對(duì)小鼠小膠質(zhì)瘤細(xì)胞BV-2的損傷作用及其炎性反應(yīng)。方法:利用體外培養(yǎng)的小膠質(zhì)細(xì)胞,給予不同濃度和作用時(shí)間的PFOS進(jìn)行染毒,通過MTT法檢測(cè)BV-2細(xì)胞活力;實(shí)時(shí)定量PCR的方法觀察誘導(dǎo)型一氧化氮合酶(inducible nitric oxide synthesis,i NOS)、白介素(interleukin,IL)-6和腫瘤壞死因子(tumor necrosis factor,TNF)-αm RNA的表達(dá)。ELISA法檢測(cè)炎性因子IL-6,免疫蛋白印跡法檢測(cè)核轉(zhuǎn)錄因子kappa B(nuclear factor-kappa B,NF-κB)等信號(hào)蛋白表達(dá)情況。結(jié)果:不同濃度PFOS對(duì)BV-2細(xì)胞染毒12、24 h后,細(xì)胞活力下降,引起明顯的細(xì)胞損傷。ELISA結(jié)果顯示,PFOS作用BV-2 24 h后,細(xì)胞炎性因子IL-6分泌增加。此外,PFOS還可引起i NOS、IL-6基因表達(dá)上調(diào),而TNF-α表達(dá)有下降趨勢(shì)。PFOS與細(xì)胞孵育后,炎性通路NF-κB明顯激活,表現(xiàn)為磷酸化程度升高。且隨染毒濃度的增加,p-NF-κB的表達(dá)有上升趨勢(shì);此外,隨染毒時(shí)間的增加,p-NF-κB的表達(dá)亦有上升趨勢(shì)。結(jié)論:PFOS可引起B(yǎng)V-2細(xì)胞活力降低,激活NF-κB通路,促進(jìn)炎性因子的釋放,該途徑可為闡明PFOS引起的神經(jīng)系統(tǒng)損傷的分子機(jī)制提供理論依據(jù)。
[Abstract]:Aim: to investigate the damage and inflammatory response of environmental endocrine disruptor perfluorooctanesulfonic acid (perfluorooctane suifonate,PFOS) to mouse microglioma cell line BV-2. Methods: microglia cultured in vitro were exposed to PFOS at different concentrations and time, and the activity of BV-2 cells was detected by MTT assay. The expression of (inducible nitric oxide synthesis,i NOS), IL-6 and (tumor necrosis factor,TNF-偽 m RNA was observed by real-time quantitative PCR, and the expression of kappa B (nuclear factor-kappa B, NF-魏 B and other signal proteins were detected by enzyme-linked immunosorbent assay (Elisa). Results: BV-2 cells were exposed to different concentrations of PFOS for 12 hours, and the cell activity decreased after 24 hours, resulting in obvious cell injury. Elisa results showed that the secretion of inflammatory factor IL-6 increased after PFOS treatment with BV-2 for 24 hours. In addition, PFOS could up-regulate the expression of I NOS,IL-6 gene, but the expression of TNF- 偽 decreased. After incubation with cells, the inflammatory pathway NF- kappa B was significantly activated, showing an increase in the degree of phosphorylation. With the increase of exposure concentration, the expression of p 鈮，

本文編號(hào)：2520826