放射人員淋巴細胞畸變檢測方法比較及人群特征分析
發(fā)布時間：2019-07-05 05:16
【摘要】：目的1.探討外周血淋巴細胞畸變?nèi)斯ら喥瑱z測法與染色體核型自動掃描成像系統(tǒng)檢測法的優(yōu)勢與劣勢。2.探索低劑量長時間接觸放射線對放射工作人員外周血淋巴細胞畸變的影響。方法1.采集唐山地區(qū)醫(yī)療放射人員外周靜脈血167例,培養(yǎng)并收集淋巴細胞,對其進行吉姆薩染色涂片,分別進行人工雙人法閱片檢測和染色體核型自動掃描成像系統(tǒng)檢測,對兩種檢測方法的檢測結果進行統(tǒng)計學比較。2.采集898例放射人員及381例非放射人員外周血,培養(yǎng)并收集淋巴細胞,對其進行吉姆薩染色涂片,以人工雙人盲法閱片,檢測淋巴細胞畸變情況。用統(tǒng)計學軟件對不同工種、不同性別、不同工齡階段的放射人員外周血淋巴細胞微核樣本陽性率、染色體畸變樣本陽性率、淋巴細胞微核率及染色體畸變率進行分析。結果1.人工組與掃描組分別檢測167例樣本,淋巴細胞畸變樣本陽性率分別為8.98%和13.17%,差異無統(tǒng)計學意義(P0.05);人工組與掃描組微核檢測中各檢測淋巴細胞167000個,掃描組微核率2.69‰高于人工組1.95‰,差異有統(tǒng)計學意義(P0.05)。兩組染色體畸變檢測中各檢測淋巴細胞33400個,掃描組染色體畸變率19.46(?)高于人工組5.399(?),差異有統(tǒng)計學意義(P0.05)。2. (1)不同工種放射人員外周血淋巴細胞畸變分析結果:工業(yè)組與醫(yī)療組放射人員外周血淋巴細胞畸變樣本陽性率分別為7.49%與8.42%,微核率分別為1.84‰與1.70‰,染色體畸變率分別為4.25(?)與5.20(?),均高于相應對照組的數(shù)值:樣本陽性率1.49%與1.12%,微核率0.37‰與0.36‰,和染色體畸變率1.73(?)與0.56(?),差異有統(tǒng)計學意義(P0.05)。但三組指標在工業(yè)組和醫(yī)療組均無統(tǒng)計學差異(P0.05)。(2)不同性別放射人員外周血淋巴細胞畸變分析結果:工業(yè)組男性與女性外周血淋巴細胞畸變樣本陽性率、微核率和染色體畸變率分別為7.58%與6.94%、1.83‰與1.85‰、4.27(?)與4.17(?),差異均無統(tǒng)計學意義(P0.05);醫(yī)療組男性與女性外周血淋巴細胞畸變樣本陽性率、微核率和染色體畸變率分別為7.83%與9.09%、1.58‰與1.83‰、6.45(?)與3.74(?),差異均無統(tǒng)計學意義(P0.05)。(3)不同工齡階段放射人員外周血淋巴細胞畸變分析結果:工業(yè)組中,淋巴細胞畸變樣本陽性率在0～9年工齡組、10～19年工齡組、20～29年工齡組和30年以上工齡組的數(shù)值分別為:1.06%、9.16%與10.48%和4.55%,統(tǒng)計學分析顯示,10～19年工齡組和20～29年工齡組高于0～9年工齡組,差異有顯著性(P0.05)。淋巴細胞微核率在0～9年工齡組、10～19年工齡組及20～29年工齡組和30年以上工齡組數(shù)值分別為0.74‰、1.94‰、1.91‰和2.02‰,經(jīng)統(tǒng)計學分析,10～19年工齡組及20～29年工齡組和30年以上工齡組高于0～9年工齡組,差異有顯著性(P0.05)。淋巴細胞染色體畸變率在4個工齡組人員數(shù)值分別為:0.53(?)、4.38(?)、6.67(?)與5.68(?),經(jīng)統(tǒng)計學分析顯示,10～19年工齡組及20～29年工齡組和30年以上工齡組高于0～9年工齡組,差異有顯著性(P0.05);醫(yī)療組中,0～9年工齡組放射人員外周血淋巴細胞微核樣本陽性率、微核率及染色體畸變率分別為1.23%、0.58‰、0.62(?),均低于10～19年工齡組8.98%、1.96‰、5.39(?),20～29年工齡組12.22%、1.99‰、6.11(?),與 30 年以上工齡組 10.61%、2.04‰、9.09(?),經(jīng)統(tǒng)計學分析,10～19年工齡組及20～29年工齡組和30年以上工齡組3組指標均高于0～9年工齡組,差異有顯著性(P0.05)。結論1.人工閱片檢測法速度較慢,存在漏檢可能,適合小樣本量樣品分析;染色體核型自動掃描分析系統(tǒng)閱片檢測法速度較快,漏檢率低,適合數(shù)目較多的樣本閱片。兩種檢測方法在外周血淋巴細胞畸變檢測樣本陽性率中差異不明顯,應互補使用。2.放射工作人員外周血淋巴細胞畸變樣本陽性率、淋巴細胞微核率、淋巴細胞染色體畸變率均高于對照組人員。長期低劑量接觸放射線可導致放射人員外周血淋巴細胞遺傳方面改變,變化程度隨工齡增加而升高。需進一步加強放射人員的職業(yè)衛(wèi)生健康防護工作。
文內(nèi)圖片：
圖片說明：染色體核型自動掃描成像系統(tǒng)
[Abstract]:Objective 1. In this paper, the advantages and disadvantages of the detection method of peripheral blood lymphocyte distortion and the detection method of autoscan imaging system of chromosome karyotype are discussed. To explore the effect of low-dose long-time exposure on the peripheral blood lymphocyte aberration of radiation workers. Method 1. The peripheral venous blood of the medical radiation personnel in the Tangshan area was collected in 167 cases, the lymphocytes were cultured and collected, and the stained smear was carried out for them, and the detection of the artificial double-person method and the automatic scanning imaging system of the chromosome were carried out, respectively. The results of the two methods of detection were statistically compared. The peripheral blood of 898 cases of radiation and 381 cases of non-radioactive personnel were collected, and the lymphocytes were cultured and collected. The positive rate of micronucleus, the positive rate of chromosome aberration, the micronucleus rate of lymphocytes and the rate of chromosome aberration were analyzed by statistical software. Results 1. The positive rates of the samples were 8.98% and 13.17%, respectively, and the difference was not significant (P <0.05). The microkernel rate of the scanning group was 2.69% higher than that of the artificial group 1.95%, respectively. The difference was significant (P0.05). In the two groups,33400 were detected in the chromosome aberration test, and the chromosome aberration rate of the scanning group was 19.46 (? ) Is higher than the manual group 5.399 (? The difference was significant (P0.05). (1) The results of the analysis of peripheral blood lymphocyte in different types of workers: The positive rate of the peripheral blood lymphocyte of the radiation personnel in the industrial group and the medical group was 7.49% and 8.42%, respectively, and the micronucleus rate was 1.84% and 1.70%, respectively, and the chromosome aberration rate was 4.25 (? ) And 5.20 (? The positive rate of the sample was 1.49% and 1.12%, the microkernel rate was 0.37% and 0.36%, and the chromosome aberration rate was 1.73 (? ) And 0.56 (? The difference was significant (P0.05). However, there was no statistical difference between the three groups in the industrial group and the medical group (P0.05). (2) The results showed that the positive rate, micronucleus rate and chromosome aberration were 7.58% and 6.94%, 1.83% and 1.85%, 4.27 (? ) And 4.17 (? The positive rate, micronucleus rate and chromosome aberration were 7.83% and 9.09%, 1.58% and 1.83%, 6.45 respectively. ) And 3.74 (? The difference was not significant (P0.05). (3) The results of the analysis of lymphocyte aberration in the peripheral blood of the radiation personnel in different service period: the positive rate of the lymphocyte aberration in the industrial group was from 0 to 9 years, and the value of the service group in the age group of 10 to 19 years, the service period of 20 to 29 years and the working length of over 30 years were: 1.06%, 9.16% and 10.48% and 4.55%, respectively. The statistical analysis showed that the working length group of 10-19 years and the working length of 20-29 years were higher than that of the working group of 0-9 years, and the difference was significant (P0.05). The lymphocyte micronucleus rate was 0.74%, 1.94%, 1.91% and 2.02% in the service group of 0 ~ 9 years, the service period of 10 ~ 19 years, and the working length of 20 ~ 29 years, respectively. The difference was significant (P0.05). The chromosome aberration rate of lymphocytes was 0.53 (?), 4.38 (?), 6.67 (? ) And 5.68 (? The results showed that the positive rate of micronucleus in the peripheral blood lymphocytes of the patients in the working group of 10 to 19 years and the working length of 20 to 29 years was higher than that of the working group of 0 to 9 years (P 0.05), and the positive rate of the micronucleus in the peripheral blood lymphocytes of the radiation personnel in the working group of 0 to 9 years in the medical group. The rate of micronucleus and chromosome aberration were 1.23%, 0.58% and 0.62 respectively. ), which is less than 8.98%, 1.96%, 5.39 (?) in the service group of 10 to 19 years, and 12.22%, 1.99% and 6.11 (?) in the service group of 20 to 29 years. ), compared with the age group of 10.61%, 2.04%, 9.09 (? The results of statistical analysis,10 to 19 years of service,20 to 29 years of service, and 3 in the service group of over 30 years were all higher than those in the age group of 0 to 9 years, and the difference was significant (P0.05). Conclusion 1. The speed of the manual reading detection method is slow, the leakage detection is possible, and the method is suitable for the sample analysis of the small sample size; the automatic scanning and analysis system of the chromosome karyotype analysis system has the advantages of high speed, low leakage detection rate and a suitable number of sample reading pieces. The difference between two detection methods in the positive rate of peripheral blood lymphocyte aberration test is not obvious, and should be used in a complementary way. The positive rate, micronucleus rate and chromosome aberration of lymphocytes in the peripheral blood of the radiation workers were higher than those in the control group. Long-term low-dose exposure to radiation can lead to changes in the genetic aspects of the peripheral blood lymphocytes of the radiation personnel, and the degree of change increases with the increase in the length of service. It is necessary to further strengthen the occupational health and health protection work of the radiological personnel.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R146
本文編號：2510269
文內(nèi)圖片：
圖片說明：染色體核型自動掃描成像系統(tǒng)
[Abstract]:Objective 1. In this paper, the advantages and disadvantages of the detection method of peripheral blood lymphocyte distortion and the detection method of autoscan imaging system of chromosome karyotype are discussed. To explore the effect of low-dose long-time exposure on the peripheral blood lymphocyte aberration of radiation workers. Method 1. The peripheral venous blood of the medical radiation personnel in the Tangshan area was collected in 167 cases, the lymphocytes were cultured and collected, and the stained smear was carried out for them, and the detection of the artificial double-person method and the automatic scanning imaging system of the chromosome were carried out, respectively. The results of the two methods of detection were statistically compared. The peripheral blood of 898 cases of radiation and 381 cases of non-radioactive personnel were collected, and the lymphocytes were cultured and collected. The positive rate of micronucleus, the positive rate of chromosome aberration, the micronucleus rate of lymphocytes and the rate of chromosome aberration were analyzed by statistical software. Results 1. The positive rates of the samples were 8.98% and 13.17%, respectively, and the difference was not significant (P <0.05). The microkernel rate of the scanning group was 2.69% higher than that of the artificial group 1.95%, respectively. The difference was significant (P0.05). In the two groups,33400 were detected in the chromosome aberration test, and the chromosome aberration rate of the scanning group was 19.46 (? ) Is higher than the manual group 5.399 (? The difference was significant (P0.05). (1) The results of the analysis of peripheral blood lymphocyte in different types of workers: The positive rate of the peripheral blood lymphocyte of the radiation personnel in the industrial group and the medical group was 7.49% and 8.42%, respectively, and the micronucleus rate was 1.84% and 1.70%, respectively, and the chromosome aberration rate was 4.25 (? ) And 5.20 (? The positive rate of the sample was 1.49% and 1.12%, the microkernel rate was 0.37% and 0.36%, and the chromosome aberration rate was 1.73 (? ) And 0.56 (? The difference was significant (P0.05). However, there was no statistical difference between the three groups in the industrial group and the medical group (P0.05). (2) The results showed that the positive rate, micronucleus rate and chromosome aberration were 7.58% and 6.94%, 1.83% and 1.85%, 4.27 (? ) And 4.17 (? The positive rate, micronucleus rate and chromosome aberration were 7.83% and 9.09%, 1.58% and 1.83%, 6.45 respectively. ) And 3.74 (? The difference was not significant (P0.05). (3) The results of the analysis of lymphocyte aberration in the peripheral blood of the radiation personnel in different service period: the positive rate of the lymphocyte aberration in the industrial group was from 0 to 9 years, and the value of the service group in the age group of 10 to 19 years, the service period of 20 to 29 years and the working length of over 30 years were: 1.06%, 9.16% and 10.48% and 4.55%, respectively. The statistical analysis showed that the working length group of 10-19 years and the working length of 20-29 years were higher than that of the working group of 0-9 years, and the difference was significant (P0.05). The lymphocyte micronucleus rate was 0.74%, 1.94%, 1.91% and 2.02% in the service group of 0 ~ 9 years, the service period of 10 ~ 19 years, and the working length of 20 ~ 29 years, respectively. The difference was significant (P0.05). The chromosome aberration rate of lymphocytes was 0.53 (?), 4.38 (?), 6.67 (? ) And 5.68 (? The results showed that the positive rate of micronucleus in the peripheral blood lymphocytes of the patients in the working group of 10 to 19 years and the working length of 20 to 29 years was higher than that of the working group of 0 to 9 years (P 0.05), and the positive rate of the micronucleus in the peripheral blood lymphocytes of the radiation personnel in the working group of 0 to 9 years in the medical group. The rate of micronucleus and chromosome aberration were 1.23%, 0.58% and 0.62 respectively. ), which is less than 8.98%, 1.96%, 5.39 (?) in the service group of 10 to 19 years, and 12.22%, 1.99% and 6.11 (?) in the service group of 20 to 29 years. ), compared with the age group of 10.61%, 2.04%, 9.09 (? The results of statistical analysis,10 to 19 years of service,20 to 29 years of service, and 3 in the service group of over 30 years were all higher than those in the age group of 0 to 9 years, and the difference was significant (P0.05). Conclusion 1. The speed of the manual reading detection method is slow, the leakage detection is possible, and the method is suitable for the sample analysis of the small sample size; the automatic scanning and analysis system of the chromosome karyotype analysis system has the advantages of high speed, low leakage detection rate and a suitable number of sample reading pieces. The difference between two detection methods in the positive rate of peripheral blood lymphocyte aberration test is not obvious, and should be used in a complementary way. The positive rate, micronucleus rate and chromosome aberration of lymphocytes in the peripheral blood of the radiation workers were higher than those in the control group. Long-term low-dose exposure to radiation can lead to changes in the genetic aspects of the peripheral blood lymphocytes of the radiation personnel, and the degree of change increases with the increase in the length of service. It is necessary to further strengthen the occupational health and health protection work of the radiological personnel.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R146
【參考文獻】
相關期刊論文 前10條
1 黃曉;李發(fā)強;;細胞自噬在植物細胞程序性死亡中的作用[J];植物學報;2016年06期
2 楊曄;薛曉英;冉玉格;周歡娣;張歌;肖志清;劉軍領;;NF-κB與放射線誘導的人食管癌細胞上皮間質轉化間的關系[J];腫瘤防治研究;2016年11期
3 李茂進;李春素;趙培青;;放射工作人員外周血淋巴細胞畸變及形態(tài)分析[J];中華勞動衛(wèi)生職業(yè)病雜志;2016年10期
4 周媛媛;王進;余寧樂;楊春勇;陳維;;江蘇省2642名放射工作人員外周血淋巴細胞染色體畸變及微核率分析[J];職業(yè)與健康;2016年14期
5 �；莘�;劉靜;趙可輝;于功昌;;我國放射工作人員外周血淋巴細胞微核檢查結果分析[J];中國輻射衛(wèi)生;2016年03期
6 裴智果;武曉東;吳云良;陳忠祥;王策;;流式細胞儀數(shù)據(jù)采集系統(tǒng)的設計方法[J];實驗室研究與探索;2016年06期
7 周璐璐;伏兵哲;許冬梅;高雪芹;;兩種化學誘變劑對沙蘆草染色體的加倍效果[J];草業(yè)科學;2016年05期
8 賴專華;張克儉;楊海峰;路建超;武永平;高濤;王悅玲;;不同工種放射工作人員輻射損傷敏感指標應用分析[J];醫(yī)學動物防制;2016年08期
9 周齊紅;俞慧娟;付風云;葉海朋;;放射工作人員外周血淋巴細胞染色體畸變和微核率分析[J];中華勞動衛(wèi)生職業(yè)病雜志;2016年04期
10 馬寶良;魯克慶;;微核試驗的改進[J];中國比較醫(yī)學雜志;2016年03期
相關碩士學位論文 前1條
1 張夢凡;低劑量γ射線誘導的淋巴細胞核質橋適應性反應及AKT的作用機制研究[D];中國疾病預防控制中心;2016年
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