【摘要】：[目的]探討職業(yè)性砷接觸工人砷甲基化代謝轉(zhuǎn)化模式與肝和皮膚損傷、長(zhǎng)鏈非編碼RNA(LncRNAs)表達(dá)的關(guān)系。[方法]2013年10月選擇云南省文山州兩個(gè)砷冶煉廠的112名工人作為接觸組,無(wú)砷接觸經(jīng)歷的居民41人為對(duì)照組。對(duì)接觸組進(jìn)行健康監(jiān)護(hù)體檢,檢查肝和皮膚損傷情況并分為4組(無(wú)損傷組、肝損傷組、皮膚損傷組、肝和皮膚均損傷組);用帶有砷預(yù)處理系統(tǒng)的原子吸收分光光度計(jì)檢測(cè)尿中無(wú)機(jī)砷、甲基砷酸和二甲基砷酸含量,并計(jì)算一、二級(jí)甲基化指數(shù);用實(shí)時(shí)熒光定量PCR法檢測(cè)外周血3種與惡性腫瘤發(fā)生關(guān)系相關(guān)的LncRNAs(MEG3、TUG1和HOTAIR)表達(dá)。[結(jié)果]職業(yè)性砷接觸工人年齡(33.9±16.8)歲,工齡(16.6±9.7)月,其中無(wú)損傷組42人,肝損傷組29人,皮膚損傷組21人,肝和皮膚均損傷組20人。各接觸組尿中3種砷化合物濃度均高于對(duì)照組(均P0.05),一、二級(jí)甲基化指數(shù)低于對(duì)照組(均P0.05);肝損傷組一級(jí)甲基化指數(shù)和皮膚損傷組一、二級(jí)甲基化指數(shù)低于肝和皮膚均損傷組(P0.05)。與對(duì)照組相比,無(wú)損傷、皮膚損傷、肝和皮膚均損傷組外周血MEG3和HOTAIR表達(dá)升高,無(wú)損傷、肝和皮膚均損傷組工人TUG1表達(dá)升高(P0.05)。與肝和皮膚均損傷組比較,無(wú)損傷和肝損傷組MEG3表達(dá)降低,肝損傷組TUG1和HOTAIR表達(dá)降低(P0.05)。[結(jié)論]職業(yè)砷接觸工人不同砷甲基化代謝模式與肝和皮膚損傷、外周血LncRNAs表達(dá)之間存在關(guān)聯(lián)。
[Abstract]:[objective] to investigate the relationship between the metabolic transformation of arsenic methylation and liver and skin injury and the expression of long chain noncoding RNA (LncRNAs) in workers exposed to occupational arsenic. [methods] in October 2013, 112 workers from two arsenic smelters in Wenshan Prefecture, Yunnan Province were selected as exposure group and 41 residents without arsenic exposure as control group. The patients in the contact group were examined for liver and skin injury and were divided into 4 groups (no injury group, liver injury group, skin injury group, liver and skin injury group). The content of inorganic arsenic, methyl arsenic acid and dimethyl arsenic acid in urine was determined by atomic absorption spectrophotometer with arsenic pretreatment system, and the first and second order methylation index was calculated. The expression of LncRNAs (MEG3,TUG1 and HOTAIR) in peripheral blood was detected by real-time fluorescence quantitative PCR. [results] the age of the workers exposed to occupational arsenic was (33.9 鹵16.8) years old and the working age was (16.6 鹵9.7) months. Among them, 42 were in the no-injury group, 29 in the liver injury group, 21 in the skin injury group, and 20 in both the liver and skin injury group. The concentrations of three arsenic compounds in urine of each exposure group were higher than those of the control group (P0.05), and the second order methylation index was lower than that of the control group (P0.05). The first methylation index and the second methylation index of liver injury group and skin injury group were lower than that of liver and skin injury group (P0.05). Compared with the control group, the expression of MEG3 and HOTAIR in peripheral blood was increased in the groups of no injury, skin injury, liver and skin injury, and the expression of TUG1 was increased in the group of liver and skin injury (P0.05). Compared with the liver and skin injury group, the expression of MEG3 was decreased in the non-injury group and the liver injury group, and the expression of TUG1 and HOTAIR was decreased in the liver injury group (P0.05). [conclusion] there is a correlation between the metabolic patterns of arsenic methylation and liver and skin injury, and the expression of LncRNAs in peripheral blood of workers exposed to arsenic.
【作者單位】： 云南省疾病預(yù)防控制中心慢性非傳染病防制所;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):81160343,30860238)
【分類(lèi)號(hào)】：R135

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