本文選題：甲基汞 + 星形膠質(zhì)細(xì)胞��； 參考：《藥物分析雜志》2015年07期

【摘要】：目的:研究氯化甲基汞(MMC)對(duì)原代培養(yǎng)的大鼠皮質(zhì)星形膠質(zhì)細(xì)胞存活和凋亡的影響。方法:MMC 0.01、0.05、0.1、0.5、1、2、4和8μmol·L-1孵育原代培養(yǎng)大鼠皮質(zhì)星形膠質(zhì)細(xì)胞3~4 h,MTT檢測(cè)星形膠質(zhì)細(xì)胞活性,Hoechst 33342熒光染色和流式細(xì)胞儀測(cè)定細(xì)胞的凋亡,JC-1熒光探針檢測(cè)線粒體膜電位,Western-blotting法測(cè)定凋亡誘導(dǎo)因子和細(xì)胞色素C的釋放。結(jié)果:MMC濃度≤0.1μmol·L-1作用0~6 h,星形膠質(zhì)細(xì)胞的活力無顯著變化;暴露時(shí)間12~24 h,細(xì)胞活力顯著降低(P0.05)。MMC濃度≥0.5μmol·L-1,星形膠質(zhì)細(xì)胞活力呈濃度依賴性和時(shí)間依賴性降低(r濃度=0.952~0.987,P0.05;r時(shí)間=0.831~0.976,P0.05);MMC濃度≤0.1μmol·L-1,隨著時(shí)間延長(zhǎng)使星形膠質(zhì)細(xì)胞凋亡率增加(P0.05)。MMC濃度≥0.5μmol·L-1作用12 h,凋亡率最高,12 h后凋亡率降低,壞死率增高(P0.05);MMC 0.5μmol·L-1呈時(shí)間依賴性降低線粒體膜電位(r=0.988,P0.05),凋亡誘導(dǎo)因子和細(xì)胞色素C的釋放增加。結(jié)論:氯化甲基汞可通過線粒體凋亡途徑誘導(dǎo)星形膠質(zhì)細(xì)胞凋亡。
[Abstract]:Aim: to study the effects of methylmercury chloride (MMC) on the survival and apoptosis of primary cultured rat cortical astrocytes. Methods the primary cultured rat cortical astrocytes were incubated with 0. 01 0. 05 and 0. 1 0. 5 mol 路L ~ (-1) and 8 渭 mol 路L ~ (-1) for 3 ~ 4 h. The activity of astrocytes was detected by Hoechst 33342 fluorescence staining and the apoptosis was detected by flow cytometry. The mitochondrial membrane potential was detected by Western-blotting with JC-1 fluorescence probe. Release of apoptosis-inducing factor and cytochrome C. Results the activity of astrocytes did not change significantly when the concentration of 0 渭 mol L-1 was less than 0.1 渭 mol L-1 for 6 h. After exposure for 12h, the cell viability decreased significantly (P 0.05N 路MMC concentration 鈮，

本文編號(hào)：2046244