本文選題：腎細胞T + 敵敵畏　； 參考：《基因組學與應用生物學》2017年03期

【摘要】：為了研究敵敵畏(dichlorvos,DDVP)對人腎細胞的毒性效應。應用MTT比色法檢測敵敵畏對人腎細胞系293T細胞增殖的影響,流式細胞儀檢測293T細胞的凋亡情況,熒光二氯熒光素二乙酸(DCF-DA)測定細胞內的活性氧(reactive oxidative species,ROS)水平。結果表明:用濃度為0.5 mmol/L、1 mmol/L、5 mmol/L、10 mmol/L、15 mmol/L和20 mmol/L的敵敵畏分別處理293T細胞1 h后發(fā)現(xiàn)敵敵畏對293T的生長抑制具有劑量效應,計算得到1 h的IC_(50)值為10.406 mmol/L;不同濃度敵敵畏(0.5 mmol/L,5 mmol/L,10 mmol/L)處理1 h 293T細胞發(fā)現(xiàn)細胞產生明顯的凋亡現(xiàn)象,隨著處理濃度的增加,細胞的凋亡率升高,檢測細胞內活性氧水平發(fā)現(xiàn)細胞內活性氧水平也隨之升高。結果提示敵敵畏能夠通過凋亡途徑抑制293T細胞的增殖,其作用機制可能是通過增加293T細胞內活性氧水平,促進細胞凋亡,進而抑制細胞增殖。
[Abstract]:To study the toxic effect of dichlorvosine DDVP on human renal cells. The effects of dichlorvos on the proliferation of human renal cell line 293T were detected by MTT colorimetry, apoptosis of 293T cells was detected by flow cytometry, and reactive oxidative speciesros were measured by fluorescence dichlorofluorescein diacetate (DCF-DAA). The results showed that dichlorvos at the concentration of 0.5 mmol / L ~ (-1) 5 mmol / L ~ (10) mmol 路L ~ (-1) and 10 mmol / L ~ (15) mmol/L and 20 mmol/L, respectively, were used to treat 293T cells for 1 h, and the results showed that dichlorvos had a dose effect on the growth inhibition of 293T cells. After 1 h treatment with 0.5 mmol / L dichlorvos (0.5 mmol / L) and 10 mmol / L (10 mmol / L) of dichlorvos, it was found that the apoptosis of 293T cells was obvious with the increase of the concentration of dichlorvos, and the apoptosis rate of the cells increased with the increase of the concentration of dichlorvos. Detection of intracellular reactive oxygen species showed that intracellular reactive oxygen species levels also increased. The results suggested that dichlorvos could inhibit the proliferation of 293T cells through the apoptotic pathway, and its mechanism might be to promote cell apoptosis by increasing the level of reactive oxygen species in 293T cells and thus inhibit cell proliferation.
【作者單位】： 貴州大學農業(yè)生物工程重點實驗室/生命科學學院;貴州大學動物科學學院;
【基金】：國家自然科學基金(No.31401091) 貴州省科學技術基金(黔科合J字[2013]2100號) 貴州大學研究生創(chuàng)新基金(No.研農2016002)共同資助
【分類號】：R114

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