發(fā)布時(shí)間：2018-05-07 01:10

  本文選題：釘螺 + 滅螺效果��； 參考：《中國血吸蟲病防治雜志》2015年06期

【摘要】：目的探索甘草提取物與氯硝柳胺聯(lián)合用藥的滅螺機(jī)制。方法采用室內(nèi)浸殺法,以氯硝柳胺(Nic)、甘草提取物(GE)、GE+Nic浸泡釘螺24、48 h后,以水養(yǎng)法檢測存活情況后,選活螺解剖,分離肝臟后觀察不同藥物對釘螺肝臟蛋白質(zhì)、糖原水平的影響。利用離體釘螺平滑肌實(shí)驗(yàn)方法觀察上述不同藥物對釘螺足跖平滑肌收縮活動(dòng)的影響。結(jié)果甘草提取物對釘螺肝臟的蛋白質(zhì)及糖原水平并無顯著影響(P均0.05),但能顯著抑制釘螺足跖平滑肌的收縮,降低收縮頻率(P均0.05);氯硝柳胺能顯著降低釘螺肝臟的蛋白質(zhì)及糖原水平(P均0.05),且可增強(qiáng)釘螺足跖平滑肌的收縮強(qiáng)度和收縮頻率(P均0.05);甘草提取物與氯硝柳胺聯(lián)用后能進(jìn)一步降低釘螺肝臟的蛋白質(zhì)及糖原水平,且能夠抑制釘螺足跖平滑肌的收縮,降低收縮頻率(P均0.05)。結(jié)論甘草提取物與氯硝柳胺聯(lián)用能加速對釘螺肝臟的損傷,同時(shí)可抑制其足跖平滑肌的活動(dòng),增加藥物接觸時(shí)間,達(dá)到滅螺增效作用。
[Abstract]:Objective to explore the snail killing mechanism of licorice extract and niclosamide. Methods the snails were soaked in nickel with nickel and licorice root extract (GE Nic) for 24 h. The survival of snails was determined by water culture method. The liver was separated and the effects of different drugs on the protein and glycogen levels in the liver were observed. The results showed that the effect of different drugs on the liver protein and glycogen level of Oncomelania hupensis was studied. The effects of the above drugs on the contractile activity of Oncomelania hupensis plantar smooth muscle were studied in vitro. Results the extract of Glycyrrhiza uralensis had no significant effect on the levels of protein and glycogen in the liver of Oncomelania hupensis, but could significantly inhibit the contraction of the smooth muscle of the foot plantaris of Oncomelania hupensis. Niclosamide could significantly decrease the protein and glycogen levels of the liver of Oncomelania hupensis, and enhance the contractile strength and contraction frequency of smooth muscle of the foot plantar of Oncomelania hupensis. Licorice extract combined with niclosamide The protein and glycogen levels in the liver of Oncomelania hupensis were further decreased. It also inhibited the contraction of metatarsal smooth muscle of Oncomelania hupensis, and decreased the contraction frequency (P < 0.05). Conclusion the combination of licorice extract and niclosamide can accelerate the damage to the liver of Oncomelania hupensis, inhibit the activity of the smooth muscle of the foot and plantar, increase the contact time of the drug, and achieve the synergistic effect of killing snails.
【作者單位】： 九江學(xué)院藥學(xué)與生命科學(xué)學(xué)院 江西省系統(tǒng)生物醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室;九江學(xué)院基礎(chǔ)醫(yī)學(xué)院;
【基金】：九江學(xué)院校級重點(diǎn)項(xiàng)目(2011KJ04);九江學(xué)院博士科研啟動(dòng)基金
【分類號】：R184.3

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本文編號：1854717