本文選題：氟化鈉 + 胞質(zhì)分裂阻滯　； 參考：《環(huán)境與職業(yè)醫(yī)學(xué)》2015年06期

【摘要】：[目的]研究氟化鈉對小鼠淋巴瘤細(xì)胞(L5178Y TK+/-3.7.2c)微核的影響及其對中國倉鼠肺成纖維細(xì)胞(CHL)細(xì)胞染色體畸變及細(xì)胞凋亡的影響。[方法]以滅菌注射用水為陰性對照,采用胞質(zhì)分裂阻滯微核細(xì)胞組學(xué)試驗(yàn)法,并以絲裂霉素(0.1μg/m L)為陽性對照,研究氟化鈉3個濃度組(100、75與50μg/m L)對L5178Y TK+/-3.7.2c細(xì)胞總微核率、核分裂指數(shù)、核質(zhì)橋發(fā)生率、核芽突發(fā)生率與含核突芽的雙核細(xì)胞數(shù)/含Ⅰ型微核與含Ⅱ型微核的雙核細(xì)胞數(shù)值的影響。以空白細(xì)胞為陰性對照,絲裂霉素(0.2μg/m L)為陽性對照,氟化鈉以600、360、216和130μg/m L的濃度作用于CHL細(xì)胞,以觀察其對CHL細(xì)胞致突變的作用。用流式細(xì)胞儀Annexin V/PI雙染法檢測氟化鈉對CHL細(xì)胞凋亡的影響。[結(jié)果]與陰性對照組的相比:1陽性對照組(絲裂霉素,0.1μg/m L)和75、100μg/m L氟化鈉組L5178Y TK+/-3.7.2c細(xì)胞微核率明顯升高(P0.05),且氟化鈉三個濃度的氟化鈉染毒組細(xì)胞核質(zhì)橋發(fā)生率、含核突芽的雙核細(xì)胞數(shù)/含Ⅰ型微核與含Ⅱ型微核的雙核細(xì)胞數(shù)值均升高(P0.01);2陽性對照組(絲裂霉素,0.2μg/m L)CHL細(xì)胞畸變率明顯升高(P0.05);氟化鈉各染毒組畸變率差異無統(tǒng)計學(xué)意義(P0.05),其早期凋亡率隨染毒濃度的增加而上升(r=0.814,P0.05)。[結(jié)論]氟化鈉對L5178Y TK+/-3.7.2c細(xì)胞染色體具有損傷作用,對CHL細(xì)胞沒有致突變作用,可以誘導(dǎo)CHL細(xì)胞的早期凋亡,呈現(xiàn)劑量相關(guān)性。
[Abstract]:[objective] to study the effect of sodium fluoride on the micronucleus of mouse lymphoma cell line L5178Y TK / -3.7.2cand its effect on chromosome aberration and apoptosis of Chinese hamster lung fibroblast (CHL) cells. [methods] the total micronucleus rate of L5178Y TK / -3.7.2c cells was studied by cytokine-blocking micronucleus assay and mitomycin 0.1 渭 g / mL positive control with sterilizing water as negative control. The total micronucleus rate of L5178Y TK / -3.7.2c cells was studied in three concentration groups of sodium fluoride, 100 渭 g / mL and 50 渭 g / mL, respectively. The effects of mitotic index, nuclear cytoplasmic bridge, nuclear bud process and the number of binuclear cells with type 鈪，

本文編號：1847499