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基于智能手機(jī)的麻痹性貝毒現(xiàn)場(chǎng)快速檢測(cè)試紙條分析儀

發(fā)布時(shí)間:2018-04-30 03:12

  本文選題:手持式分析儀 + 智能手機(jī)。 參考:《傳感技術(shù)學(xué)報(bào)》2017年12期


【摘要】:石房蛤毒素(STX)是常見的麻痹性貝毒(PSP),通過食物鏈積累在貝類食品中,人類若食用這種有毒的貝類會(huì)影響健康,甚至中毒。因此迫切需要一種簡便的方法來檢測(cè)這種麻痹性貝毒以避免中毒事件的發(fā)生;诟(jìng)爭性免疫原理的試紙條可以和石房蛤毒素產(chǎn)生特異性顯色反應(yīng),采用研發(fā)的新型手持式分析儀進(jìn)行檢測(cè)分析。毒素分析儀采用智能手機(jī)作為光探測(cè)器,配合3D打印配件,再通過智能手機(jī)上研發(fā)的用于圖像采集和數(shù)據(jù)處理的應(yīng)用程序,可對(duì)顯色后的試紙條進(jìn)行分析處理并得出所測(cè)毒素的濃度。這種方法簡單、快速、檢出限低、靈敏度高。目前,本儀器可測(cè)得的STX的檢出限:5.2 ng/m L,檢測(cè)范圍:5.2 ng/m L~100 ng/m L。實(shí)驗(yàn)表明,本文研制的基于智能手機(jī)系統(tǒng)和免疫分析的手持式試紙條分析儀有望成為對(duì)麻痹性貝毒進(jìn)行現(xiàn)場(chǎng)快速檢測(cè)的有效工具。
[Abstract]:STX is a common paralytic shellfish poison, which accumulates in shellfish food through the food chain. If humans eat this poisonous shellfish, it will affect their health and even be poisoned. Therefore, a simple method is urgently needed to detect the paralytic shellfish poison to avoid poisoning. The test strip based on the principle of competitive immunity can produce specific color reaction with clam toxin, and a new hand-held analyzer is developed for detection and analysis. The toxin analyzer uses a smart phone as a photodetector, along with 3D printing accessories, and then uses an application developed on the smartphone for image acquisition and data processing. The color-developing test strip can be analyzed and treated and the concentration of the toxin can be obtained. This method is simple, rapid, low detection limit and high sensitivity. At present, the detection limit of STX measured by this instrument is 1: 5.2 ng/m / L, and the detection range is 1: 5.2 ng/m / L ~ (100 ng/m / L). The experimental results show that the hand-held test strip analyzer based on smart phone system and immune analysis is expected to be an effective tool for rapid detection of paralytic shellfish virus in the field.
【作者單位】: 浙江大學(xué)生物傳感器國家專業(yè)實(shí)驗(yàn)室生物醫(yī)學(xué)工程教育部重點(diǎn)實(shí)驗(yàn)室生儀學(xué)院;中國科學(xué)院傳感技術(shù)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】:國家海洋公益專項(xiàng)項(xiàng)目(201305010)
【分類號(hào)】:R155.3;TH83

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