發(fā)布時間：2018-04-19 06:01

  本文選題：脂肪組織 + 間質(zhì)干細(xì)胞�。� 參考：《中國組織工程研究》2017年33期

【摘要】：背景:研究表明,骨髓間充質(zhì)干細(xì)胞具有在體外分化為肺泡上皮細(xì)胞的可能性,但至今未見脂肪間充質(zhì)干細(xì)胞與肺泡上皮細(xì)胞通過長時間Transwell共培養(yǎng),誘導(dǎo)脂肪間充質(zhì)干細(xì)胞分化的相關(guān)報道。目的:觀察大鼠肺泡Ⅱ型上皮細(xì)胞系RLE-6TN誘導(dǎo)大鼠脂肪間充質(zhì)干細(xì)胞分化為肺泡Ⅱ型上皮細(xì)胞的可行性。方法:取3只SPF級健康雌性SD大鼠作為供體,分離、提取、培養(yǎng)、鑒定脂肪間充質(zhì)干細(xì)胞。實(shí)驗(yàn)分2組:實(shí)驗(yàn)組(脂肪間充質(zhì)干細(xì)胞與大鼠肺泡Ⅱ型上皮細(xì)胞系RLE-6TN進(jìn)行Transwell共培養(yǎng))和對照組(脂肪間充質(zhì)干細(xì)胞單獨(dú)培養(yǎng))。共培養(yǎng)第21天,倒置顯微鏡觀察各組脂肪間充質(zhì)干細(xì)胞的形態(tài)變化,對脂肪間充質(zhì)干細(xì)胞進(jìn)行SP-C免疫熒光染色,熒光顯微鏡下觀察蛋白表達(dá)并拍照,采用Image pro plus 6.0軟件分析熒光表達(dá)的積分吸光度值。結(jié)果與結(jié)論:(1)共培養(yǎng)21 d,實(shí)驗(yàn)組的脂肪間充質(zhì)干細(xì)胞形態(tài)由長梭形、成纖維細(xì)胞樣,逐漸轉(zhuǎn)化為卵圓形、多邊形,對照組脂肪間充質(zhì)干細(xì)胞形態(tài)保持成纖維細(xì)胞樣;(2)熒光顯微鏡下觀察,實(shí)驗(yàn)組脂肪間充質(zhì)干細(xì)胞可見紅色熒光表達(dá),對照組無紅色熒光表達(dá),實(shí)驗(yàn)組的熒光表達(dá)較對照組增強(qiáng)(P0.05);(3)結(jié)果表明,大鼠肺泡Ⅱ型上皮細(xì)胞系RLE-6TN能夠在長時間(21 d)共培養(yǎng)條件下,誘導(dǎo)脂肪間充質(zhì)干細(xì)胞分化為肺泡上皮細(xì)胞。
[Abstract]:Background: studies have shown that bone marrow mesenchymal stem cells can differentiate into alveolar epithelial cells in vitro, but no adipose mesenchymal stem cells and alveolar epithelial cells have been co-cultured through Transwell for a long time.Related reports on inducing adipose mesenchymal stem cell differentiation.Aim: to observe the feasibility of differentiation of rat adipose mesenchymal stem cells into alveolar type 鈪，

本文編號：1771868