本文關鍵詞： Fe 氧化應激 肺表面蛋白C　出處：《毒理學雜志》2015年01期 　論文類型：期刊論文

【摘要】：目的大氣細顆粒所含的過渡金屬元素與呼吸道疾病的發(fā)生密切相關,但其誘導的氧化應激對呼吸道細胞的影響仍不十分清楚。本文主要研究過渡金屬元素Fe誘導的肺泡上皮細胞氧化應激對肺表面蛋白C(SP-C)表達的影響。方法肺泡II型上皮MLE-12細胞經Fe暴露處理24 h后,使用噻唑藍(MTT)、2,7-二氯熒光素(DCF)和流式細胞測定細胞活力、細胞內活性氧(ROS)生成和細胞凋亡;采用實時定量PCR和Western blot分析細胞的SP-C mRNA和蛋白表達水平變化。結果 Fe明顯抑制MLE-12細胞活力(P0.01),升高細胞內的ROS生成水平(P0.01),并上調細胞SP-C mRNA和蛋白的表達水平。但其表達水平被過氧化氫酶、乙酰-L-半胱氨酸和磺酸去鐵敏預處理后明顯降低。結論 Fe誘導生成的ROS參與SP-C的表達。
[Abstract]:Objective the transition metal elements contained in fine particles of the atmosphere are closely related to the occurrence of respiratory diseases. However, the effect of oxidative stress on respiratory tract cells was still unclear. The effect of oxidative stress on the expression of lung surface protein (CnSP-C) in alveolar epithelial cells induced by transition metal element Fe was studied in this paper. The epithelial MLE-12 cells were exposed to Fe for 24 h. The cell viability, intracellular reactive oxygen species (Ros) production and apoptosis were measured by MTT and flow cytometry. Real time quantitative PCR and Western blot were used to analyze the expression of SP-C mRNA and protein in MLE-12 cells. Results Fe significantly inhibited the activity of MLE-12 cells, increased the level of ROS production and up-regulated the expression of SP-C mRNA and protein. Its expression level was expressed by catalase, After pretreatment with acetyl-L-cysteine and deferrite sulfonic acid, the expression of SP-C was significantly decreased. Conclusion Fe induced ROS is involved in the expression of SP-C.
【作者單位】： 中國科學院大學生命科學學院環(huán)境與健康研究室;中國農業(yè)大學生物學院;
【基金】：國家自然科學基金委面上項目(21377127,11275264,U1432245,J1103520)
【分類號】：R114

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相關期刊論文 前1條

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相關期刊論文 前10條

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本文編號：1544977