【摘要】：目的:觀察骨髓神經(jīng)組織定向干細(xì)胞(NTCSCs)移植對噪聲性聾聽神經(jīng)損傷豚鼠耳蝸毛細(xì)胞的修復(fù)作用。方法:將50只豚鼠按隨機(jī)數(shù)字表法分為對照組(10只)、噪聲暴露組(10只)和移植組(30只)。移植組及噪聲暴露組豚鼠均在白噪聲下暴露1周,暴露結(jié)束后將轉(zhuǎn)染綠色熒光蛋白(EGFP)基因的骨髓NTCSCs移植至豚鼠左耳耳蝸蝸軸處(右耳作為自身對照,注射相同體積的0.1%PBS溶液),分別測試噪聲暴露前1d和暴露后1、2及4周后的ABR閾值,并通過掃描電鏡觀察噪聲暴露后4周豚鼠耳蝸毛細(xì)胞的形態(tài)學(xué)變化情況。結(jié)果:成功分離培養(yǎng)出骨髓NTCSCs,隨著移植時(shí)間的延長,轉(zhuǎn)染EGFP的骨髓NTCSCs球團(tuán)和細(xì)胞聚集現(xiàn)象,逐漸接近c(diǎn)orti器和基膜;耳蝸切片中Nestin(+)細(xì)胞的數(shù)量明顯增多(P0.05),而MyosinⅦa(+)細(xì)胞的數(shù)量無明顯變化。與噪聲暴露前相比,移植組豚鼠ABR閾移小于噪聲暴露組(均P0.05)。掃描電鏡顯示,噪聲暴露組豚鼠耳蝸內(nèi)、外毛細(xì)胞均出現(xiàn)聽毛紊亂、融合及缺失;而移植組耳蝸病變較輕,聽毛僅有輕微倒伏、融合現(xiàn)象。結(jié)論:隨著骨髓NTCSCs移植時(shí)間的延長,豚鼠ABR閾值得到明顯改善。骨髓NTCSCs移植后可逐漸分化成耳蝸螺旋神經(jīng)節(jié)神經(jīng)元,進(jìn)而起到改善噪聲性聾豚鼠聽力的作用。
[Abstract]:Aim: to observe the effect of transplantation of bone marrow neural tissue-oriented stem cells (NTCSCs) on cochlear hair cells of guinea pigs with noise-induced deaf nerve injury. Methods: 50 guinea pigs were randomly divided into three groups: control group (n = 10), noise exposure group (n = 10) and transplantation group (n = 30). The guinea pigs in the transplantation group and noise exposure group were exposed to white noise for 1 week. After the exposure, the bone marrow NTCSCs transfected with green fluorescent protein (EGFP) gene was transplanted to the cochlea axis of the left ear (right ear as a self-control). The ABR thresholds were measured 1 day before noise exposure and 1, 2 and 4 weeks after noise exposure. The morphological changes of cochlear hair cells in guinea pigs were observed by scanning electron microscopy (SEM) at 4 weeks after noise exposure. Results: bone marrow NTCSCs, was successfully isolated and cultured. With the prolongation of transplantation time, the phenomenon of NTCSCs pellet and cell aggregation in bone marrow transfected with EGFP was gradually close to that of Corti organ and basement membrane. The number of Nestin () cells in cochlea sections increased significantly (P0.05), but the number of Myosin 鈪，

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